Bacterial chemotaxis in static gradients quantified in a biopolymer membrane-integrated microfluidic platform

Piao, Hongyu; Ly, Khanh L.; Pham, Le P H; Pottash, Alex Eli; Sheridan, Kathleen; Wu, Hsuan‐Chen; Tsao, Chen‐Yu; Quan, David N.; Bentley, William E.; Rubloff, Gary W.; Sintim, Herman O.; Luo, Xiaolong

doi:10.1039/d2lc00481j

Cited by 8 publications

(5 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They typically can be categorized as one of two types. In the first, the CG is set up solely through analyte diffusion, which has the disadvantage of often needing hours to establish a gradient that then continuously changes in time, which can complicate the interpretation of experimental results [ 43 , 44 ]. The second type uses advection to continuously supply source and sink fluids to the CG region [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration

et al. 2023

View full text Add to dashboard Cite

We demonstrate a method to effectively 3D print microfluidic devices with high-resolution features using a biocompatible resin based on avobenzone as the UV absorber. Our method relies on spectrally shaping the 3D printer source spectrum so that it is fully overlapped by avobenzone’s absorption spectrum. Complete overlap is essential to effectively limit the optical penetration depth, which is required to achieve high out-of-plane resolution. We demonstrate the high resolution in practice by 3D printing 15 μm square pillars in a microfluidic chamber, where the pillars are separated by 7.7 μm and are printed with 5 μm layers. Furthermore, we show reliable membrane valves and pumps using the biocompatible resin. Valves are tested to 1,000,000 actuations with no observable degradation in performance. Finally, we create a concentration gradient generation (CG) component and utilize it in two device designs for cell chemotaxis studies. The first design relies on an external dual syringe pump to generate source and sink flows to supply the CG channel, while the second is a complete integrated device incorporating on-chip pumps, valves, and reservoirs. Both device types are seeded with adherent cells that are subjected to a chemoattractant CG, and both show clear evidence of chemotactic cellular migration. Moreover, the integrated device demonstrates cellular migration comparable to the external syringe pump device. This demonstration illustrates the effectiveness of our integrated chemotactic assay approach and high-resolution biocompatible resin 3D printing fabrication process. In addition, our 3D printing process has been tuned for rapid fabrication, as printing times for the two device designs are, respectively, 8 and 15 min.

show abstract

Section: Resultsmentioning

confidence: 99%

Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Antibiotics with strong interaction with alginate hydrogel such as tetracycline [ 48 ] were excluded. It has been previously shown that small molecules such as fluorescein, glucose, peptides, and antibiotics can freely diffuse through the alginate hydrogels [ 34 , 35 , [49] , [50] , [51] ] as the pore size of the alginate hydrogel prepared with 1% w/v should be around tens of nanometers [ 52 , 53 ]. Together, these observations suggest that the alginate hydrogel did not limit the diffusion of the selected antibiotics.…”

Section: Resultsmentioning

confidence: 99%

Dissolvable alginate hydrogel-based biofilm microreactors for antibiotic susceptibility assays

Pham

Colon-Ascanio

et al. 2023

Biofilm

Self Cite

View full text Add to dashboard Cite

“…It should be noted that the current system has a limited experimental window of 30 min before the gradient is dissipated, which is shorter than the time frames used to study behaviour in static gradients. 55 However, static systems require continuous replenishment of source and sink, which adds complexity to the experimental setup.…”

Section: Planariansmentioning

confidence: 99%

3D printed porous membrane integrated devices to study the chemoattractant induced behavioural response of aquatic organisms

Kalathil Balakrishnan,

Schultz,

Lee

et al. 2024

Lab Chip

View full text Add to dashboard Cite

show abstract

Bacterial chemotaxis in static gradients quantified in a biopolymer membrane-integrated microfluidic platform

Abstract: Chemotaxis is a fundamental bacterial response mechanism to changes in chemical gradients of specific molecules known as chemoattractant or chemorepellent. The advancement of biological platforms for bacterial chemotaxis research is...

Cited by 8 publications

References 63 publications

Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration

Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration

Dissolvable alginate hydrogel-based biofilm microreactors for antibiotic susceptibility assays

3D printed porous membrane integrated devices to study the chemoattractant induced behavioural response of aquatic organisms

Contact Info

Product

Resources

About