Bacterial Colonization and Degradation of Demineralized Dentin Matrix in situ

Strijp, A.J.P. van; Steenbergen, T. J. M. van; Graaff, J. de; Cate, J.M. ten

doi:10.1159/000261615

Cited by 34 publications

(32 citation statements)

References 17 publications

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“…Oral bacteria may release proteolytic enzymes that degrade the collagenous dentine matrix. In a study investigating the colonization and degradation of similar dentine specimens [Van Strijp et al, 1994], gelatinolytic strains were found in the predominant microflora. Although no correlation was found between the loss of collagen and the presence of these strains, the involvement of bacterial enzymes in the process of dentine degradation cannot be excluded.…”

Section: Discussionmentioning

confidence: 99%

“…In the past the role of bacterial enzymes has been investigated. Although bacterial strains that produce proteolytic enzymes were found in carious dentine, a direct action towards degradation of dentine collagen remained questionable [Van Strijp et al, 1994. However, bacterial products such as acids and proteinases could be indirectly involved in matrix degradation by activating host-derived proteinases [Uitto and Raeste, 1978;Sorsa et al, 1992].…”

mentioning

confidence: 99%

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Host-Derived Proteinases and Degradation of Dentine Collagen in situ

Strijp¹,

Jansen

DeGroot³

et al. 2003

Caries Res

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151

123

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Dentine root caries is a process of demineralization and degradation of the organic matrix by proteinases. In this in situ study, the presence and activity of the matrix metalloproteinases 1, 2 and 9 (MMP-1, MMP-2, MMP-9) in saliva and in completely demineralized dentine specimens were investigated. Furthermore, the activity of cathepsin B was determined in saliva. A correlation between these enzymes and the level of degraded collagen was investigated. Demineralized dentine specimens were mounted in the partial prosthesis of 17 volunteers. Saliva samples were taken at 0, 2 and 4 weeks. After 4 weeks, the enzymes were extracted from the dentine specimens and the collagen loss was assessed. The collagen loss varied between 0 and 40.3%. Zymography of the saliva and the dentine extract samples showed that (pro-)MMP-2 and (pro-)MMP-9 were present. The levels of active MMPs were assessed, using fluorogenic MMP-specific substrates. All but 3 of the 51 saliva samples showed MMP-1 activity ranging from 1.5 to 101.1 relative fluorescence units (RFU)/s. Forty-eight saliva samples showed gelatinolytic MMP-2/MMP-9 activity (1.7–141.1 RFU/s). MMP-1 activity was shown in all dentine extracts varying between 3.5 and 295.0 RFU/s. From the dentine extracts, 15 showed MMP-2/MMP-9 activity (0.2–13.7 RFU/s). The MMP activity from both saliva and dentine extracts did not correlate with the collagen loss. The activity of salivary cathepsin B varied from 4.8 to 42.2 arbitrary units/min. A positive correlation was found between salivary MMP activity and cathepsin B activity. This study revealed that gelatinolytic enzyme activity was present both in saliva and dentine collagen. No correlation could be observed, however, between the level of enzyme activity and the collagen loss of the dentine specimens.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Host-Derived Proteinases and Degradation of Dentine Collagen in situ

Strijp¹,

Jansen

DeGroot³

et al. 2003

Caries Res

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151

123

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“…Once demineralisation of the dentine has been initiated other bacteria can migrate into the developing lesion. Generally, current treatments of caries attempt to remove all infected tissue but this cannot be guaranteed [van Strijp et al, 1994;Bjørndal et al, 1997]. However Kidd et al [1996] have proposed that the removal of only the softened and wet dentine is necessary, the effective sealing of the cavity with a restorative being suffi cient to achieve 'healing of the lesion' as the bacteria become quiescent.…”

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confidence: 99%

The Photo-Activated Antibacterial Action of Toluidine Blue O in a Collagen Matrix and in Carious Dentine

Williams¹,

Pearson²,

Colles³

et al. 2004

Caries Res

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The main aim of this study was to determine the susceptibility to photo-activated disinfection (PAD) of Streptococcus mutans when the organism was present in a collagen matrix – an environment similar to that which would exist within a carious tooth. In addition, the susceptibility to PAD of bacteria present in carious human teeth was also determined. Light was delivered to the collagen and teeth using a system comprising a 0.8-mm diameter isotropic tip emitting light at 633 ± 2 nm. A single concentration of TBO (10 µg/ml) was used with both collagen and dentine. Two contact times, 30 and 180 s, were evaluated in intact collagen and additionally, for 180 s only, in collagen partially disrupted by shredding. The effect of energy doses from 1.8 to 14.4 J on the kills attained was assessed by determining the number of surviving viable bacteria. In carious dentine, two contact times, 30 and 60 s and one energy dose, 4.8 J, were used. Antibacterial effects were less than those obtained using planktonic suspensions with a maximum mean log reduction of 1.4 in shredded collagen and dentine. Increasing contact time increased the antibacterial effectiveness in both substrates although this was not always of statistical significance. Shredding the collagen resulted in significantly increased bacterial kills compared to those obtained in intact collagen for the 30-second contact time. The collagen matrix appeared to be a suitable model for carious dentine with advantages of availability and reproducibility. The results of this study have shown that PAD can achieve appreciable kills of oral bacteria, including S. mutans, when the organisms are embedded in a collagen gel or are present in carious teeth.

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“…References has previously been studied by Armstrong [1958Armstrong [ , 1961, Goldberg and Keil [1989], van Strijp et al [1992van Strijp et al [ , 1994 and Tjäderhane et al [1998]. Although not directly comparable, these published results did not show a total degradation of carious dentin.…”

mentioning

confidence: 77%

Pronase Digestion of Carious Dentin

1999

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The aim of this study was to investigate the individual capabilities of the proteolytic enzyme preparation Pronase™, the enzyme collagenase and sodium hypochlorite to disintegrate and solubilize carious dentin. Samples of carious dentin, and samples of sound dentin for comparison, were extracted 4 times in succession for 24 h with buffered solutions of Pronase. Separate carious dentin samples were extracted in the same manner with buffered solutions of collagenase or with aqueous sodium hypochlorite. The extracts, the solid residues left over after the extractions and untreated samples of carious and sound dentin were digested with sulfuric acid–H₂O₂ and then analyzed for nitrogen content by a special adaptation of the Berthelot color reaction. Although Pronase did not attack sound dentin, it solubilized more than 90% of the nitrogen present in carious dentin. Collagenase solubilized approximately 66% of the nitrogen, whereas sodium hypochlorite released only 12–20% of the nitrogen of carious dentin. In clinical dentistry, chemical disintegration of carious dentin may reduce the need for mechanical removal of sound tooth structure.

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Bacterial Colonization and Degradation of Demineralized Dentin Matrix in situ

Cited by 34 publications

References 17 publications

Host-Derived Proteinases and Degradation of Dentine Collagen in situ

Host-Derived Proteinases and Degradation of Dentine Collagen in situ

The Photo-Activated Antibacterial Action of Toluidine Blue O in a Collagen Matrix and in Carious Dentine

Pronase Digestion of Carious Dentin

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