Bacterial Communication Systems

Harder, Tilmann; Rice, Scott A.; Dobretsov, Sergey; Thomas, Torsten; Carré‐Mlouka, Alyssa; Kjelleberg, Staffan; Steinberg, Peter D.; McDougald, Diane

doi:10.1002/9783527681501.ch07

Cited by 4 publications

(7 citation statements)

References 104 publications

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“…In spite of the low bacterial population in the open sea and the low chemical stability of AHLs at the high pH of seawater, new evidence reinforces the idea of the importance of AHL-mediated QS mechanisms in marine environments [ 3 , 5 , 13 , 14 ]. More recently, numerous studies have reported the isolation of AHL-producing bacterial strains from marine samples [ 15 , 16 , 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 84%

“…The data obtained from the Mediterranean Sea bacterial collections obtained from 90 and 2000 m depths showed a high percentage of strains with the ability to activate the AHL-reporter A. tumefaciens NTL4. Although the culture conditions could strongly affect QS signal production, and the detection of AHL-like activity in the cultures does not guarantee that the signals are produced under environmental conditions [ 5 ], the results suggest that AHL-mediated QS systems could be a common coordination mechanism in marine bacterial communities. It should be noted that the microtiter-based screening method does not allow high growth rates, and, therefore, the number of AHL producers could be underestimated, at least under laboratory conditions, as demonstrated for the identification of additional positive strains when cultured in higher-volume, shaken cultures.…”

Section: Discussionmentioning

confidence: 99%

“…These QS signal molecules are constituted by a homoserine lactone ring (HSL) linked by an amide bond to a fatty acid (between 4 and 18 carbons). AHLs are QS signals that are considered typical of Gram-negative bacteria [ 3 ], although they are also produced by different clades of bacteria [ 4 , 5 ], including the Gram-positive Exiguobacterium sp. [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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High Prevalence of Quorum-Sensing and Quorum-Quenching Activity among Cultivable Bacteria and Metagenomic Sequences in the Mediterranean Sea

Muras

López‐Pérez

Mayer

et al. 2018

Genes

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There is increasing evidence being accumulated regarding the importance of N-acyl homoserine lactones (AHL)-mediated quorum-sensing (QS) and quorum-quenching (QQ) processes in the marine environment, but in most cases, data has been obtained from specific microhabitats, and subsequently little is known regarding these activities in free-living marine bacteria. The QS and QQ activities among 605 bacterial isolates obtained at 90 and 2000 m depths in the Mediterranean Sea were analyzed. Additionally, putative QS and QQ sequences were searched in metagenomic data obtained at different depths (15–2000 m) at the same sampling site. The number of AHL producers was higher in the 90 m sample (37.66%) than in the 2000 m sample (4.01%). However, the presence of QQ enzymatic activity was 1.63-fold higher in the 2000 m sample. The analysis of putative QQ enzymes in the metagenomes supports the relevance of QQ processes in the deepest samples, found in cultivable bacteria. Despite the unavoidable biases in the cultivation methods and biosensor assays and the possible promiscuous activity of the QQ enzymes retrieved in the metagenomic analysis, the results indicate that AHL-related QS and QQ processes could be common activity in the marine environment.

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Section: Introductionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

High Prevalence of Quorum-Sensing and Quorum-Quenching Activity among Cultivable Bacteria and Metagenomic Sequences in the Mediterranean Sea

Muras

López‐Pérez

Mayer

et al. 2018

Genes

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“…More diversity was observed in areas with silty nutrient‐rich sediments (e.g., Pensacola Bay area) compared to sandy nutrient‐poor regions (e.g., Pensacola beach and Fort Pickens). These data could suggest that with greater diversity, the greater the potential for the production of potent secondary metabolites by the microbes (via quorum sensing to regulate the expression of virulence factors) for the sake of survival (Harder et al., ; Persson et al., ). In the case of the latter, the pathogenic Bacillus flexus appeared as the prevalent species and it exhibited cytotoxic activity in our assay.…”

Section: Discussionmentioning

confidence: 99%

Identification and bioactive potential of marine microorganisms from selected Florida coastal areas

Christensen

Martin

2017

MicrobiologyOpen

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The ocean, with its rich untapped chemical biodiversity, continues to serve as a source of potentially new therapeutic agents. The evaluation of the diversity of cultivable microorganisms from the marine sponge Halichondria panicea and ocean sediment samples were examined and their potential as sources of antimicrobial and antiproliferative agents were investigated. The marine sponge and sediments were collected at different depths (0.9–6 meters) and locations in Florida, including Florida Keys, Port St. Joe in Pensacola, Pensacola Bay, Pensacola Beach, and Fort Pickens. Twenty‐one cultivatable isolates were grouped according to their morphology and identified using 16S rRNA molecular taxonomy. The bacterial community identified consisted of members belonging to the Actinobacteria, Bacteroidetes, Proteobacteria (Alpha‐ and Gamma‐classes) and Firmicutes phylogeny. Seven of the microbes exhibited mild to significant cytotoxic activities against five microbial indicators but no significant cytotoxic activities were observed against the pancreatic (PANC‐1) nor the multidrug‐resistant ovarian cancer cell lines (NCI/ADR). This work reaffirms the phyla Actinobacteria and Proteobacteria as sources of potential bioactive natural product candidates for drug discovery and development.

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“…Similar effects have also been reported in the green alga Ulva lactua (Rasmussen and Givskov, ). Notably, QQ activity was found in about 25% of ∼280 extracts from marine organisms (reviewed by Harder et al ., ), demonstrating the potential for the further identification of specific QS antagonists from marine organisms (Skindersoe et al ., ).…”

Section: Manipulation Of Qs‐dependent Pathways By Plantsmentioning

confidence: 99%

Silencing the mob: disrupting quorum sensing as a means to fight plant disease

Helman

Chernin

2014

Molecular Plant Pathology

120

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Bacteria are able to sense their population's density through a cell-cell communication system, termed 'quorum sensing' (QS). This system regulates gene expression in response to cell density through the constant production and detection of signalling molecules. These molecules commonly act as auto-inducers through the up-regulation of their own synthesis. Many pathogenic bacteria, including those of plants, rely on this communication system for infection of their hosts. The finding that the countering of QS-disrupting mechanisms exists in many prokaryotic and eukaryotic organisms offers a promising novel method to fight disease. During the last decade, several approaches have been proposed to disrupt QS pathways of phytopathogens, and hence to reduce their virulence. Such studies have had varied success in vivo, but most lend promising support to the idea that QS manipulation could be a potentially effective method to reduce bacterial-mediated plant disease. This review discusses the various QS-disrupting mechanisms found in both bacteria and plants, as well as the different approaches applied artificially to interfere with QS pathways and thus protect plant health.

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Bacterial Communication Systems

Cited by 4 publications

References 104 publications

High Prevalence of Quorum-Sensing and Quorum-Quenching Activity among Cultivable Bacteria and Metagenomic Sequences in the Mediterranean Sea

High Prevalence of Quorum-Sensing and Quorum-Quenching Activity among Cultivable Bacteria and Metagenomic Sequences in the Mediterranean Sea

Identification and bioactive potential of marine microorganisms from selected Florida coastal areas

Silencing the mob: disrupting quorum sensing as a means to fight plant disease

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