Bacterial Communities Associated with Crude Oil Bioremediation through Composting Approaches with Indigenous Bacterial Isolate

Abstract

In this study, we aim to investigate the efficiency of crude oil bioremediation through composting and culture-assisted composting. First, forty-eight bacteria were isolated from a crude oil-contaminated soil, and the isolate with the highest crude oil degradation activity, identified as Pseudomonas aeruginosa, was selected. The bioremediation was then investigated and compared between crude oil-contaminated soil (S), the contaminated soil composted with fruit-based waste (SW), and the contaminated soil composted with the same waste with the addition of the selected bacterium (SWB). Both compost-based methods showed high efficiencies of crude oil bioremediation (78.1% and 83.84% for SW and SWB, respectively). However, only a slight difference between the treatments without and with the addition of P. aeruginosa was observed. To make a clear understanding of this point, bacterial communities throughout the 4-week bioremediation period were analyzed. It was found that the community dynamics between both composted treatments were similar, which corresponds with their similar bioremediation efficiencies. Interestingly, Pseudomonas disappeared from the system after one week, which suggests that this genus was not the key degrader or only involved in the early stage of the process. Altogether, our results elaborate that fruit-based composting is an effective approach for crude oil bioremediation.

Bio-Drying of Municipal Wastewater Sludge: Effects of High Temperature, Low Moisture Content and Volatile Compounds on the Microbial Community

“…According to a study by Steger et al [53], fungi and actinobacteria usually proliferated during periods of relatively low temperatures (<45 • C). Similar data on the dominance of the genus Prevotella during composting a mixture of soil with fruit waste have also been reported [54]. Changes in temperature conditions and pH led to decrease in the initial amount of Prevotella and Simplicispira members.…”

“…Piceno et al [55] noted that in the thermophilic stage of WS composting, these genera were not detected, although they were found in the initial mixture. It was reported that members of Prevotella, which prevail in the soil, take an active part in the degradation process and get into the compost mixture through the materials used [54]. The genus Shuttleworthia, which was in the original biomass, usually inhabits the activated sludge.…”

Evaluating Bacterial Population Changes and Ecological Dynamics in Oil-Impacted Soils Using 16S rRNA Amplicon Sequencing

“…Only the genus Prevotella and Aerococcus could be directly associated with positive ecosystem services such as biodegradation of xenobiotic and recalcitrant pollutants. Prevotella's primary involvement in crude oil degradation was rst reported by Mukjang, et al (2022), and Aerococcus has also been known to be present in hydrocarbon-contaminated environments (Biswas, et al 2022; Dey, Das, and Kazy 2018) suggest a targeted approach in bioremediation strategies, yet the e ciency and speci city of these bacteria in different hydrocarbon types remain unclear. Interestingly, many bacterial genera of animal origin, associated with human and animal gut microbiota were found to have increased in relative abundance.…”

Biotechnological Potential of Oil-Tolerant Strains for Possible Use in Bioremediation

“…It is a vital living ecosystem that offers a habitat to plants, animals and humans [1,2]. However, soil degradation, a significant global problem, exacerbated by petroleum hydrocarbon industry-related contamination, is posing a serious threat to living organisms [3,4]. The increasing global demand for energy has led to a surge in the consumption of oil and its products.…”