Bacterial Communities in Areas of Oil and Methane Seeps in Pelagic of Lake Baikal

Zakharenko, А. S.; Galachyants, Yuriy P; Морозов, И. В.; Shubenkova, O. V.; Morozov, A. A.; Ivanov, V. G.; Pimenov, Nikolai V.; Krasnopeev, Andrey; Zemskaya, Т. I.

doi:10.1007/s00248-018-1299-5

Cited by 19 publications

(7 citation statements)

References 96 publications

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“…Notably, the phylum Gracilibacteria in the domain Bacteria was also identified, and was highly abundant via specific 16S rRNA primers for methanogens (data not shown). This finding was supported by high levels of Gracilibacteria in the methane-enriched environmental samples [37,38], and similarities between the genome sequences of Gracilibacteria and those of archaea [39,40]. Our results showed that Methanobrevibacter was the most abundant genus in the rumen archaeal community of ruminants [5], such as goats [20], cattle [41,42], yaks [15] and impala [43].…”

Section: Discussionsupporting

confidence: 72%

Characterization of the Rumen Microbiota and Volatile Fatty Acid Profiles of Weaned Goat Kids under Shrub-Grassland Grazing and Indoor Feeding

Guo

Liu

et al. 2020

Animals

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In this study, we conducted comparative analyses to characterize the rumen microbiota and volatile fatty acid (VFA) profiles of weaned Nanjiang Yellow goat kids under shrub-grassland grazing (GR), shrub-grassland grazing and supplementary feeding (SF), and indoor feeding (IF) systems. We observed significant differences (p < 0.05) in the concentrations of total VFA and the proportions of acetate and butyrate in the rumen fluid among the three groups, whereas the proportions of propionate and the acetate/propionate ratio did not differ substantially. Alpha diversity of the rumen bacterial and archaeal populations in the GR and SF kids was significantly higher (p < 0.05) than that in the IF goat kids, and significant differences (p < 0.05) in similarity were observed in the comparisons of GR vs. IF and SF vs. IF. The most predominant bacterial phyla were Bacteroidetes and Firmicutes across the three groups, and the archaeal community was mainly composed of Euryarchaeota. At the genus and species levels, the cellulose-degrading bacteria, including Lachnospiraceae, Ruminococcaceae and Butyrivibrio fibrisolvens, were abundant in the GR and SF groups. Furthermore, 27 bacterial and 11 unique archaeal taxa, such as Lachnospiraceae, Butyrivibrio fibrisolvens, and Methanobrevibacter ruminantium, were identified as biomarkers, and showed significantly different (p < 0.05) abundances among the three groups. Significant Spearman correlations (p < 0.05), between the abundances of several microbial biomarkers and the concentrations of VFAs, were further observed. In summary, our results demonstrated that the adaptation to grazing required more rumen bacterial populations due to complex forage types in shrub-grassland, although the rumen fermentation pattern did not change substantially among the three feeding systems. Some microbial taxa could be used as biomarkers for different feeding systems, particularly cellulose-degrading bacteria associated with grazing.

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Section: Discussionsupporting

confidence: 72%

Characterization of the Rumen Microbiota and Volatile Fatty Acid Profiles of Weaned Goat Kids under Shrub-Grassland Grazing and Indoor Feeding

Guo

Liu

et al. 2020

Animals

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“…Ca. Gracilibacteria members (formerly known as GN02/BD1-5) have been previously detected in diverse extreme or CH 4 -rich environments (e.g., hypersaline microbial mat, deep-sea hydrothermal vents, thermal springs) [ 70 , 71 , 72 , 73 , 74 , 75 , 76 ]. In field and groundwater CH 4 -spiked mesocosms studies, the growth of Ca.…”

Section: Discussionmentioning

confidence: 99%

Procaryotic Diversity and Hydrogenotrophic Methanogenesis in an Alkaline Spring (La Crouen, New Caledonia)

et al. 2021

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(1) Background: The geothermal spring of La Crouen (New Caledonia) discharges warm (42 °C) alkaline water (pH~9) enriched in dissolved nitrogen with traces of methane, but its microbial diversity has not yet been studied. (2) Methods: Cultivation-dependent and -independent methods (e.g., Illumina sequencing and quantitative PCR based on 16S rRNA gene) were used to describe the prokaryotic diversity of this spring. (3) Results: Prokaryotes were mainly represented by Proteobacteria (57% on average), followed by Cyanobacteria, Chlorofexi, and Candidatus Gracilibacteria (GN02/BD1-5) (each > 5%). Both potential aerobes and anaerobes, as well as mesophilic and thermophilic microorganisms, were identified. Some of them had previously been detected in continental hyperalkaline springs found in serpentinizing environments (The Cedars, Samail, Voltri, and Zambales ophiolites). Gammaproteobacteria, Ca. Gracilibacteria and Thermotogae were significantly more abundant in spring water than in sediments. Potential chemolithotrophs mainly included beta- and gammaproteobacterial genera of sulfate-reducers (Ca. Desulfobacillus), methylotrophs (Methyloversatilis), sulfur-oxidizers (Thiofaba, Thiovirga), or hydrogen-oxidizers (Hydrogenophaga). Methanogens (Methanobacteriales and Methanosarcinales) were the dominant Archaea, as found in serpentinization-driven and deep subsurface ecosystems. A novel alkaliphilic hydrogenotrophic methanogen (strain CAN) belonging to the genus Methanobacterium was isolated, suggesting that hydrogenotrophic methanogenesis occurs at La Crouen.

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“…Среди культивируемых углеводородокисляющих микроорганизмов доминирующие положение занимают представители филумов Proteobacteria и Actinobacteria. Полученные данные согласуются с результатами изучения структуры микробных сообществ водной толщи, донных осадков и битумных построек с помощью метода высокопроизводительного секвенирования гена 16S рРНК, согласно которым микробное сообщество в основном представлено филумами Actinobacteria, Cyanobacteria и Proteobacteria с доминированием бактерий р. Rhodococcus, участвующих в биодеградации ароматических углеводородов и н-алканов нефти [Ломакина и др., 2014;Likhoshvay et al, 2013;Zemskaya et al, 2015;Zakharenko et al, 2018]. Способность деградировать углеводороды, вероятно, закреплена в геномах байкальских микроорганизмов, обитающих в районе естественного выхода нефти [Ломакина и др., 2014;Likhoshvay et al, 2013], так как вследствие геологических процессов нефть и продукты ее преобразования относятся к постоянным компонентам экосистемы восточного побережья центральной котловины озера, образование которых произошло в олигоцен -миоцене [Конторович и др., 2007].…”

Section: результатыunclassified

Современное Состояние Глубоководного Выхода Нефти У М. Горевой Утес (Средний Байкал)

2019

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Bacterial Communities in Areas of Oil and Methane Seeps in Pelagic of Lake Baikal

Cited by 19 publications

References 96 publications

Characterization of the Rumen Microbiota and Volatile Fatty Acid Profiles of Weaned Goat Kids under Shrub-Grassland Grazing and Indoor Feeding

Characterization of the Rumen Microbiota and Volatile Fatty Acid Profiles of Weaned Goat Kids under Shrub-Grassland Grazing and Indoor Feeding

Procaryotic Diversity and Hydrogenotrophic Methanogenesis in an Alkaline Spring (La Crouen, New Caledonia)

Современное Состояние Глубоководного Выхода Нефти У М. Горевой Утес (Средний Байкал)

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