Bacterial communities in digestive and excretory organs of cicadas

Wang, Dandan

doi:10.1007/s00203-019-01763-4

Cited by 12 publications

(15 citation statements)

References 102 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…mongolica and in the digestive and excretory organs of cicada Su. yangi [27,28]. However, our results revealed that neither Sulcia nor YLS were harbored in the salivary glands and gut tissues in the two sampled cicada species (Figure 5 and Table S1).…”

Section: Distribution Of Symbionts In Different Tissues Of Cicadasmentioning

confidence: 69%

“…mongolica [27]. Another study based on Illumina genome sequencing and fluorescence in situ hybridization (FISH) microscopy reported that Sulcia distributes in the digestive and excretory organs beside the bacteriomes and gonads in cicada Subpsaltria yangi [28]. However, most previous studies have shown that Sulcia is harbored in the bacteriomes, which can be transovarially transmitted to the offspring in auchenorrhynchan insects [9,10,17].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Study on Symbiotic Systems of Cicadas Provides New Insights into Distribution of Microbial Symbionts and Improves Fluorescence In Situ Hybridization Technique

Huang

Zhou

Zhang

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Nutritional symbionts of sap-sucking auchenorrhynchan insects of Hemiptera are usually confined to the bacteriomes and/or fat bodies. Knowledge is limited about the distribution of microbial symbionts in other organs. We investigated the distribution of obligate symbionts in the salivary glands, gut tissues, reproductive organs, bacteriomes, and fat bodies of two cicada species, Karenia caelatata and Tanna sp., using integrated methods, including a modified fluorescence in situ hybridization (FISH) technique, which can greatly enhance the FISH signal intensity of related symbionts. We revealed that Candidatus Sulcia muelleri (Sulcia) and a yeast-like fungal symbiont (YLS) were harbored in the bacteriomes and fat bodies, respectively. Both of Sulcia and YLS can be transmitted to the offspring via ovaries, forming a “symbiont ball” in each egg. Neither Sulcia nor YLS were harbored in the salivary glands, gut tissues and testes. Phylogenetic trees of both Sulcia and cicadas confirm that K. caelatata is a member of the tribe Dundubiini, and the tribe Leptopsaltriini that comprises Ta. sp. is not monophyletic. YLS of K. caelatata is embedded inside the lineage of YLS of Dundubiini, whereas YLS of Ta. sp. is closely related to the clade comprising both cicada-parasitizing fungi Ophiocordyceps and YLS of Mogannia conica and Meimuna mongolica, suggesting an evolutionary replacement of YLS in Ta. sp. from an Ophiocordyceps fungus to another Ophiocordyceps fungus. Our results provide new insights into the symbiosis between Cicadidae and related symbionts. Modification through the addition of helpers and heat shock greatly enhanced the FISH signal intensity of YLS, which may provide guidelines for enhancement of the hybridization signal intensity of other symbiont(s) in the FISH experiments.

show abstract

Section: Distribution Of Symbionts In Different Tissues Of Cicadasmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

A Study on Symbiotic Systems of Cicadas Provides New Insights into Distribution of Microbial Symbionts and Improves Fluorescence In Situ Hybridization Technique

Huang

Zhou

Zhang

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The nutritional function and metabolic versatility of bacterial symbionts were also featured in some auchenorrhynchan insects (5,24,25). However, knowledge about the bacterial communities and distribution of obligate and facultative symbionts residing in cicadas is quite limited, although bacterial communities of the alimentary canal and some other organs of a few cicada species have been investigated (34)(35)(36)(37). To date, nothing is known about the transmission patterns of secondary symbionts in cicadas.…”

mentioning

confidence: 99%

Transovarial Transmission of Bacteriome-Associated Symbionts in the Cicada Pycna repanda (Hemiptera: Cicadidae)

Huang

Wang

et al. 2020

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Although transovarial transmission of bacteriome-associated symbionts in hemipteran insects is extremely important for maintaining intimate host-symbiont associations, our knowledge of cellular mechanisms underlying the transmission process is quite limited. We investigated bacterial communities of salivary glands, bacteriomes, and digestive and reproductive organs and clarified the transovarial transmission of bacteriome-associated symbionts of the mountain-habitat specialist Pycna repanda using integrated methods. The bacterial communities among different gut tissues and those of bacteriomes of males and females both show similarity, whereas differences are exhibited among bacterial communities in testes and ovaries. The primary symbionts “Candidatus Sulcia muelleri” (hereafter “Ca. Sulcia”) and “Candidatus Hodgkinia cicadicola” (hereafter “Ca. Hodgkinia”) were not only restricted to but also dominant in the bacteriomes and ovaries. “Ca. Hodgkinia” cells in the bacteriomes of both sexes exhibited different colors by histological and electron microscopy. Also considering the results of a restriction fragment length polymorphism (RFLP)-based cloning approach, we hypothesize that “Ca. Hodgkinia” may have split into cytologically different cellular lineages within this cicada species. Regarding the dominant secondary symbionts, Rickettsia was detected in the salivary glands, digestive organs, and testes, whereas Arsenophonus was detected in the bacteriomes and ovaries. Our results show that Arsenophonus can coexist with “Ca. Sulcia” and “Ca. Hodgkinia” within bacteriomes and can be transovarially transmitted with these obligate symbionts together from mother to offspring in cicadas, but it is not harbored in the cytoplasm of “Ca. Sulcia.” The change in the shape of “Ca. Sulcia” and “Ca. Hodgkinia” during the transovarial transmission process is hypothesized to be related to the limited space and novel microenvironment. IMPORTANCE Cicadas establish an intimate symbiosis with microorganisms to obtain essential nutrients that are extremely deficient in host plant sap. Previous studies on bacterial communities of cicadas mainly focused on a few widely distributed species, but knowledge about mountain-habitat species is quite poor. We initially revealed the physical distribution of the primary symbionts “Ca. Sulcia” and “Ca. Hodgkinia” and the dominant secondary symbionts Rickettsia and Arsenophonus in the mountain-habitat specialist Pycna repanda and then clarified the transovarial transmission process of bacteriome-associated symbionts in this species. Our observations suggest that “Ca. Hodgkinia” may have split into cytologically distinct lineages within this cicada species, and related cicadas might have developed complex mechanisms for the vertical transmission of the bacteriome-associated symbionts. We also revealed that Arsenophonus can be transovarially transmitted in auchenorrhynchan insects when it is not harbored in the cytoplasm of other endosymbionts. Our results highlight transovarial transmission mechanisms of bacteriome-associated symbionts in sap-feeding insects.

show abstract

“…Although the presence of bacterial cells in adult spittlebug’s gut has already been highlighted by microscopy studies [ 26 , 27 ], and gut endosymbionts have been reported for other Auchenorrhyncha species [ 35 , 36 ], knowledge on the gut microbiota of Aphrophoridae species is still inadequate.…”

Section: Discussionmentioning

confidence: 99%

Diversity of the Bacterial Community Associated with Hindgut, Malpighian Tubules, and Foam of Nymphs of Two Spittlebug Species (Hemiptera: Aphrophoridae)

et al. 2023

View full text Add to dashboard Cite

Spittlebugs are xylem-sap feeding insects that can exploit a nutrient-poor diet, thanks to mutualistic endosymbionts residing in various organs of their body. Although obligate symbioses in some spittlebug species have been quite well studied, little is known about their facultative endosymbionts, especially those inhabiting the gut. Recently, the role played by spittlebugs as vectors of the phytopathogenetic bacterium Xylella fastidiosa aroused attention to this insect group, boosting investigations aimed at developing effective yet sustainable control strategies. Since spittlebug nymphs are currently the main target of applied control, the composition of gut bacterial community of the juveniles of Philaenus spumarius and Lepyronia coleoptrata was investigated using molecular techniques. Moreover, bacteria associated with their froth, sampled from different host plants, were studied. Results revealed that Sodalis and Rickettsia bacteria are the predominant taxa in the gut of P. spumarius and L. coleoptrata nymphs, respectively, while Rhodococcus was found in both species. Our investigations also highlighted the presence of recurring bacteria in the froth. Furthermore, the foam hosted several bacterial species depending on the host plant, the insect species, or on soil contaminant. Overall, first findings showed that nymphs harbor a large and diverse bacterial community in their gut and froth, providing new accounts to the knowledge on facultative symbionts of spittlebugs.

show abstract

Bacterial communities in digestive and excretory organs of cicadas

Cited by 12 publications

References 102 publications

A Study on Symbiotic Systems of Cicadas Provides New Insights into Distribution of Microbial Symbionts and Improves Fluorescence In Situ Hybridization Technique

A Study on Symbiotic Systems of Cicadas Provides New Insights into Distribution of Microbial Symbionts and Improves Fluorescence In Situ Hybridization Technique

Transovarial Transmission of Bacteriome-Associated Symbionts in the Cicada Pycna repanda (Hemiptera: Cicadidae)

Diversity of the Bacterial Community Associated with Hindgut, Malpighian Tubules, and Foam of Nymphs of Two Spittlebug Species (Hemiptera: Aphrophoridae)

Contact Info

Product

Resources

About