Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system

Cardona, Emilie; Gueguen, Yannick; Magré, Kévin; Lorgeoux, Bénédicte; Piquemal, David; Pierrat, Fabien; Noguier, Florian; Saulnier, Denis

doi:10.1186/s12866-016-0770-z

Cited by 217 publications

(189 citation statements)

References 40 publications

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“…; Cardona et al . ). Present investigations also revealed significant ( P < 0·05) increase in microbial flora of Firmicutes , where genus Lactobacillus was enriched in water after Bio‐ball treatment.…”

Section: Resultsmentioning

confidence: 97%

Biological ball filters regulate bacterial communities in marron ( Cherax cainii ) culture system

Foysal

Fotedar

Gupta

et al. 2019

Lett Appl Microbiol

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This study aimed to characterize the bacterial communities in rearing water treated with commercial plastic biological ball filters named as Bio‐ball in marron culture for 60 days. Inclusion of Bio‐ball in the aquaculture tanks showed improvement in water quality parameters and enrichment of bacterial communities in terms of operational taxonomic units. The water treated with Bio‐ball showed significantly less nitrate, nitrite, ammonia, phosphorus and high dissolved oxygen concentration than untreated control group. At phylum level, Proteobacteria was dominant in both control and treated water, whereas Firmicutes was found to be significantly (P < 0·05) enriched in Bio‐ball treated water. Among the classified genus, Aquabacterium and Polunucleobacter were most dominant in control and Bio‐ball treated water respectively. Linear Discriminant Analysis Effect Size exhibited 31 indicator bacterial genus, 10 in control and 21 in treated condition, suggesting the enrichment of microbial lineages with addition of Bio‐ball. The bacteria Haliscomenobacter, Hypnocyclicus, Pajaroellobacter and Vibrio were found to be significantly (P < 0·001) correlated with higher pH, nitrate, nitrite, phosphorus and ammonia in control tanks, whereas Corynebacterium was linked to higher temperature in treated water. Overall results suggest that Bio‐ball filter media significantly improved the water quality and microbial populations in aquaculture tanks. Significance and Impact of the Study The results of this study revealed the positive impacts of Bio‐ball in enrichment of microbial flora associated with the degradation process of nitrogenous and organic compounds. Bio‐ball also showed the capability to prevent the colonization of harmful bacteria, and favoured the growth of beneficial microbes in aquatic system. This study therefore could pave the ways of increasing the aquaculture production by improving the water quality.

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Section: Resultsmentioning

confidence: 97%

Biological ball filters regulate bacterial communities in marron ( Cherax cainii ) culture system

Foysal

Fotedar

Gupta

et al. 2019

Lett Appl Microbiol

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“…Water and sediment, which are important components of the culture environment, are closely related to the growth of shrimp (Cardona et al, ; Del'Duca, ; Wang et al, ). A previous study showed that microorganisms, bacterium and microalgae could accumulate, grow and multiply on the surface of BWPG and gradually form a complex biofilm structure (Zhou et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Two grams surface sediment samples were used to precipitate microorganisms via oscillation, incubation and centrifugation. After 24 hr of fasting, sixty healthy shrimps of same size, body colour and body surface were randomly collected from the two ponds and were divided into three groups randomly (Cardona et al, ). Shrimp intestine samples were then removed from the abdominal cavities with a sterile tweezer.…”

Section: Methodsmentioning

confidence: 99%

“…Amplification of the 16S rRNA gene was carried out using the primers (16S rRNA V4 515F: 5′‐GTGCCAGCM GCCGCGGTAA‐3′) and 16S rRNA V4 806R: 5′‐GGACTACHVGGGTWTCTAAT‐3′) with a standard PCR protocol (Cardona et al, ). The PCR amplification program was performed as following: 98°C for 3 min, 30 cycles, where 1 cycle was consisted of 98°C for 45 s (denaturation), 55°C for 45 s (annealing), 72°C for 45 s (extension), and a final extension at 72°C for 7 min.…”

Section: Methodsmentioning

confidence: 99%

“…Reports of studies on microorganisms in aquaculture have mainly been confined to the composition of microbial community in culture water (Zhao et al, ), sediment (Santander‐De Leon et al, ) and shrimp intestine (Li et al, ; Liu et al, ; Zhang et al, ). Cardona and colleagues reported that rearing‐water bacterial communities influenced the microbiota of shrimp intestine (Cardona et al, ). Indeed, the rearing environment and other factors modified the microbiota of shrimp intestine (Chaiyapechara et al, ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of biological water purification grid on microbial community of culture environment and intestine of the shrimpLitopenaeus vannamei

et al. 2019

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Biological water purification grid (BWPG), a type of biofilm, could increase aquaculture production by improving the growth of shrimp and culture environment. In this study, the microbial community composition and diversity of water, sediment and shrimp intestine were analysed using the Illumina Miseq high‐throughput sequencing technique. Installation of BWPG did not only increase the dissolved oxygen, pH and decreased inorganic nutrients, but also increased shrimp body length by 5.39% and weight by 16.35%. Furthermore, it was found that the microbial community diversity of water and shrimp intestine in test pond with BWPG was higher than that of control pond without BWPG. Cyanobacteria, Bacillus and Lactococcus were enriched in the test pond with BWPG. However, Rhodobacter was mainly identified in the test pond. It thus seems to suggest that the application of BWPG in shrimp culture pond enhanced the microbial species richness, types of species and proportion of beneficial bacteria in culture environment as well as shrimp intestine. The results from this study will therefore provide some scientific basis for the improvement and development of shrimp aquaculture.

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Biofloc technology as part of a sustainable aquaculture system: A review on the status and innovations for its expansion

McCusker

Bolton‐Warberg

Davies

et al. 2023

Aquaculture Fish & Fisheries

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Increased food demand, reflecting a rising global human population, attaining 8 billion in 2022, has furthered the intensification of farmed aquatic animal production. Intensification practices can be resource demanding for micro and macronutrients, dedicated feeds, fossil fuels, chemotherapeutics, and water. Water replaces evaporative losses and may be used to dilute nutrient‐rich wastewater. As an alternative to wastewater discharge, biofloc technology (BFT) uses microbes to manage nutrient levels in production systems. A microbial community grown at high densities can assimilate waste metabolites for growth. This is promoted by increasing the carbon–nitrogen (C:N) ratio using a combination of aquafeed fed to the fish and an additional carbohydrate source, for example, starch, molasses, or bran. The microbial biomass offers a continuous and additional food source to the farmed animal, thus reducing the need for finished aquafeeds and lowering the feed conversion ratio. Although the approach relies on multiple interacting species, BFT can be deployed in relatively low technology settings. This review considers the basis of BFT and identifies areas for innovation and expansion. For example, control of light quantity and quality can influence the biofloc and hence the growth of cultured species. Research on biofloc aquaculture is dominated by studies using tilapia and shrimp, but the technology could be applied to other species, particularly species that are tolerant of biofloc conditions and not highly carnivorous. Biofloc may be a useful biosecurity tool for all or part of the life cycle, and meals made from dried biofloc may enhance the production of cultured species. The key benefits of BFT can potentially be seen in reduced water consumption, lower feed requirements, and improved fish health. This review differs from current review papers in proposing the use of a life cycle assessment in conjunction with BFT, which may be a useful tool for describing and communicating the relative benefits of biofloc systems and their wider environmental impact. This study will serve as a useful knowledge base of BFT information for students, researchers, and stakeholders alike, offering a central source of the main aspects around the culturing of biofloc, key parameters, common species used, areas of potential improvement, and a discussion on where the future of BFT may lie.

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Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system

Cited by 217 publications

References 40 publications

Biological ball filters regulate bacterial communities in marron ( Cherax cainii ) culture system

Biological ball filters regulate bacterial communities in marron ( Cherax cainii ) culture system

Effects of biological water purification grid on microbial community of culture environment and intestine of the shrimpLitopenaeus vannamei

Biofloc technology as part of a sustainable aquaculture system: A review on the status and innovations for its expansion

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