Bacterial community composition differs with species and toxigenicity of the diatom Pseudo-nitzschia

Guannel, Michele Lynne; Horner-Devine, M. Claire; Rocap, Gabrielle

doi:10.3354/ame01513

Cited by 54 publications

(49 citation statements)

References 81 publications

(137 reference statements)

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“…Assuming P. australis as the only DA producer in these samples, cellular DA content was 14.6-32.3 pg cell À1 , whereas if total Pseudo-nitzschia cells are considered these cell quotas would be reduced to 11.0-21.2 pg cell À1 . In either case, these quotas represent the higher values observed during our study and are in the range of the highest DA cell content measured in P. australis cultures (22, 27 and 37 pg cell À1 ) by Guannel et al (2011), Cusack et al (2002 and Garrison et al (1992), respectively.…”

Section: Discussionsupporting

confidence: 65%

Species occurrence of the potentially toxigenic diatom genus Pseudo-nitzschia and the associated neurotoxin domoic acid in the Argentine Sea

et al. 2017

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Keywords:Amnesic shellfish poisoning South-western Atlantic Pseudo-nitzschia diversity P. australis P. brasiliana Domoic acid A B S T R A C TThe marine diatom genus Pseudo-nitzschia, the major known producer of the neurotoxin domoic acid (DA) responsible for the amnesic shellfish poisoning (ASP) syndrome in humans and marine mammals, is globally distributed. The genus presents high species richness in the Argentine Sea and DA has been frequently detected in the last few years in plankton and shellfish samples, but the species identity of the producers remains unclear. In the present work, the distribution and abundance of Pseudo-nitzschia species and DA were determined from samples collected on two oceanographic cruises carried out through the Argentine Sea ($39-47 S) during summer and spring 2013. Phytoplankton composition was analysed by light and electron microscopy while DA was determined by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The genus Pseudo-nitzschia was recorded in 71 and 86% of samples collected in summer and spring, respectively, whereas DA was detected in only 42 and 21% of samples, respectively. Microscopic analyses revealed at least five potentially toxic species (P. australis, P. brasiliana, P. fraudulenta, P. pungens, P. turgidula), plus putatively non-toxigenic P. dolorosa, P. lineola, P. turgiduloides and unidentified specimens of the P. pseudodelicatissima complex. The species P. australis showed the highest correlation with DA occurrence (r = 0.55; p < 0.05), suggesting its importance as a major DA producer in the Argentine Sea. In the northern area and during summer, DA was associated with the presence of P. brasiliana, a species recorded for the first time in the Argentine Sea. By contrast, high concentrations of P. fraudulenta, P. pungens and P. turgidula did not correspond with DA occurrence. This study represents the first successful attempt to link toxigenicity with Pseudo-nitzschia diversity and cell abundance in field plankton populations in the south-western Atlantic.

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Section: Discussionsupporting

confidence: 65%

Species occurrence of the potentially toxigenic diatom genus Pseudo-nitzschia and the associated neurotoxin domoic acid in the Argentine Sea

et al. 2017

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“…Production of domoic acid appears to require direct contact between the diatom host and bacteria (Kobayashi et al 2009). Spe cific bacteria are associated with different Pseudo-nitzschia species (Stewart et al 1997) and bacterial associates appear to vary with the toxigenicity of the host diatom (Guannel et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Exploring bacteria–diatom associations using single-cell whole genome amplification

Baker¹,

Kemp²

2014

Aquat. Microb. Ecol.

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Diatoms are responsible for a large fraction of oceanic and freshwater biomass production and are critically important for sequestration of carbon to the deep ocean. As with most surfaces present in aquatic systems, bacteria colonize the exterior of diatom cells, and they interact with the diatom and each other. The ecology of diatoms may be better explained by conceptualizing them as composite organisms consisting of the host cell and its bacterial associates. Such associations could have collective properties that are not predictable from the properties of the host cell alone. Past studies of these associations have employed culture-based, whole-population methods. In contrast, we examined the composition and variability of bacterial assemblages attached to individual diatoms. Samples were collected in an oligotrophic system (Station ALOHA, 22°45' N, 158°00' W) at the deep chlorophyll maximum. Forty eukaryotic host cells were isolated by flow cytometry followed by multiple displacement amplification, including 26 Thalassiosira spp., other diatoms, dinoflagellates, coccolithophorids, and flagellates. Bacteria were identified by amplifying, cloning, and sequencing 16S rDNA using primers that select against chloroplast 16S rDNA. Bacterial sequences were recovered from 32 of 40 host cells, and from parallel samples of the free-living and particle-associated bacteria. Bacterial assemblages varied substantially even among closely related host cells. Host cells and the free-living and particle-associated samples can be placed into distinct groups based on the phylogenetic relatedness of their associated bacteria, rather than the identity of the host cell. As yet, the functional implications of these groups are unknown.KEY WORDS: Bacterioplankton diversity · Marine diatoms · Algal-bacterial interaction · Micro environment · Multiple displacement amplification · Single-cell Resale or republication not permitted without written consent of the publisher

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“…The recent finding that the bacterial community composition differs between DA-producing and non-DA-producing Pseudo-nitzschia spp. may be related to DA and, if so, suggests that DA may act as a bacterial deterrent against unwanted or algicidal bacteria and/or, conversely, may encourage the growth of favorable bacteria (66). To better understand the interplay between bacteria and DA biosynthesis, the effect of DA on bacterial diversity needs to be investigated, especially given recent evidence that some bacteria may be able to degrade DA (69).…”

Section: Synergistic Interactionsmentioning

confidence: 99%

Interactions between Diatoms and Bacteria

Amin

Parker

Armbrust

2012

Microbiol Mol Biol Rev

837

741

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SUMMARY Diatoms and bacteria have cooccurred in common habitats for hundreds of millions of years, thus fostering specific associations and interactions with global biogeochemical consequences. Diatoms are responsible for one-fifth of the photosynthesis on Earth, while bacteria remineralize a large portion of this fixed carbon in the oceans. Through their coexistence, diatoms and bacteria cycle nutrients between oxidized and reduced states, impacting bioavailability and ultimately feeding higher trophic levels. Here we present an overview of how diatoms and bacteria interact and the implications of these interactions. We emphasize that heterotrophic bacteria in the oceans that are consistently associated with diatoms are confined to two phyla. These consistent bacterial associations result from encounter mechanisms that occur within a microscale environment surrounding a diatom cell. We review signaling mechanisms that occur in this microenvironment to pave the way for specific interactions. Finally, we discuss known interactions between diatoms and bacteria and exciting new directions and research opportunities in this field. Throughout the review, we emphasize new technological advances that will help in the discovery of new interactions. Deciphering the languages of diatoms and bacteria and how they interact will inform our understanding of the role these organisms have in shaping the ocean and how these interactions may change in future oceans.

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Bacterial community composition differs with species and toxigenicity of the diatom Pseudo-nitzschia

Cited by 54 publications

References 81 publications

Species occurrence of the potentially toxigenic diatom genus Pseudo-nitzschia and the associated neurotoxin domoic acid in the Argentine Sea

Species occurrence of the potentially toxigenic diatom genus Pseudo-nitzschia and the associated neurotoxin domoic acid in the Argentine Sea

Exploring bacteria–diatom associations using single-cell whole genome amplification

Interactions between Diatoms and Bacteria

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