Bacterial Community Diversity and Bacterial Interaction Network in Eight Mosquito Species

Abstract

Mosquitoes (Diptera: Culicidae) are found widely throughout the world. Several species can transmit pathogens to humans and other vertebrates. Mosquitoes harbor great amounts of bacteria, fungi, and viruses. The bacterial composition of the microbiota of these invertebrates is associated with several factors, such as larval habitat, environment, and species. Yet little is known about bacterial interaction networks in mosquitoes. This study investigates the bacterial communities of eight species of Culicidae collected in Vale do Ribeira (Southeastern São Paulo State) and verifies the bacterial interaction network in these species. Sequences of the 16S rRNA region from 111 mosquito samples were analyzed. Bacterial interaction networks were generated from Spearman correlation values. Proteobacteria was the predominant phylum in all species. Wolbachia was the predominant genus in Haemagogus leucocelaenus. Aedes scapularis, Aedes serratus, Psorophora ferox, and Haemagogus capricornii were the species that showed a greater number of bacterial interactions. Bacterial positive interactions were found in all mosquito species, whereas negative correlations were observed in Hg. leucocelaenus, Ae. scapularis, Ae. serratus, Ps. ferox, and Hg. capricornii. All bacterial interactions with Asaia and Wolbachia were negative in Aedes mosquitoes.

Bacterial Community Survey of Wolbachia-Infected Parthenogenetic Parasitoid Trichogramma pretiosum (Hymenoptera: Trichogrammatidae) Treated with Antibiotics and High Temperature

“…Microbiome analysis found that Wolbachia and Acinetobacter were the major core members of the microbiome among these treatments. Though Acinetobacter is not capable of enhancing the biotic fitness of the host [60], it is abundant in mosquito [61] and in arrhenotokous parasitoid, Diglyphus wani [62]. The core member of Acinetobacter should be the result of the high rates of multidrug resistance and extensive drug resistance [63].…”

Diverse novel Wolbachia bacteria strains and genera-specific co-infections with Asaia bacteria in Culicine mosquitoes from ecologically diverse regions of Cameroon

“…There are several cases where Wolbachia and Asaia have been detected in the same mosquito (e.g. Schrieke et al 2022 Computational and Structural Biotechnology 1 , Hegde et al 2018 Frontiers in Microbiology 2 , da Silva et al 2022 genes 3 , Chen et al 2020 Frontiers in Microbiology 4 ) but they may not be occupying the same tissues, especially given that Asaia can be environmentally acquired and often resides in the gut. This study also does not provide sufficient evidence for a Wolbachia-Asaia coinfection.…”