Bacterial community mapping of the intestinal tract in acute pancreatitis rats based on 16S rDNA gene sequence analysis

Tao, Xufeng; Guo, Fengjin; Zhou, Qi; Hu, Fenglin; Xiang, Hong; Xiao, Gary Guishan; Shang, Dong

doi:10.1039/c8ra09547g

Cited by 15 publications

(10 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The observed species, Shannon index, Simpson index, Chao1 index, ACE index and Good‐coverage were calculated with QIIME version 1.7.0. Observed species were evaluated to reveal the number of OTUs in the samples (Tao et al, 2019). The ACE and Chao1 indexes reveal the richness of the bacteria communities, whereas Shannon and Simpson's indexes indicate the diversity of the bacteria communities (Han et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Application of biodegradable polymers as carbon sources in ex situ biofloc systems: Water quality and shift of microbial community

Kokkuar

Srisapoome

et al. 2021

Aquaculture Research

View full text Add to dashboard Cite

Aquaculture systems are becoming more intensive to increase economic efficiency (Ren et al., 2019). Large amounts of feed are put into the systems to enhance the growth of cultured animals, which leads to an elevated concentrations of organic waste from uneaten feed and faeces. Ammonia is produced when those organic waste is decomposed (Li & Boyd, 2016). The conversion methods for removing ammonia nitrogen in aquaculture systems are photoautotrophic removal by algae, autotrophic bacterial conversion of ammonia to nitrate and heterotrophic bacterial conversion of ammonia-nitrogen directly to microbial biomass (Dauda, 2020;El-Sayed, 2020). None of the three pathways exist alone, and the dominant one depends on the management of the aquaculture systems (Dauda, 2020).

show abstract

Section: Methodsmentioning

confidence: 99%

Application of biodegradable polymers as carbon sources in ex situ biofloc systems: Water quality and shift of microbial community

Kokkuar

Srisapoome

et al. 2021

Aquaculture Research

View full text Add to dashboard Cite

show abstract

“…Beta-diversity was analyzed to evaluate the differences of samples in the complexity of species by the software QIIME (Version 1.8.0). Distance-based statistical tests (weighted and unweighted UniFrac distances clustered) had been applied to test the association of the cluster composition with environmental and biological covariates [27] .…”

Section: Methodsmentioning

confidence: 99%

Tyrosine supplement ameliorates murine aGVHD by modulation of gut microbiome and metabolome

Lin

et al. 2020

EBioMedicine

View full text Add to dashboard Cite

Background Microbial communities and their metabolic components in the gut are of vital importance for immune homeostasis and have an influence on the susceptibility of the host to a number of immune-mediated diseases like acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, little is known about the functional connections between microbiome and metabolome in aGVHD due to the complexity of the gastrointestinal environment. Method Initially, gut microbiota and fecal metabolic phenotype in aGVHD murine models were unleashed by performing 16S ribosomal DNA gene sequencing and ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS)-based metabolomics. Findings The group with aGVHD experienced a significant drop in Lachnospiraceae_unclassified but an increase in the relative abundance of Clostridium XI , C lostridium XIVa and Enterococcus . Meanwhile, a lower content of tyrosine was observed in the gut of aGVHD mice. The correlation analysis revealed that tyrosine-related metabolites were inversely correlated with Clostridium XIVa , besides, Blautia and Enterococcus also displayed the negative tendency in aGVHD condition. Apart from exploring the importance and function of tyrosine, different tyrosine diets were offered to mice during transplantation. Additional tyrosine supplements can improve overall survival, ameliorate symptoms at the early stage of aGVHD and change the structure and composition of gut microbiota and fecal metabolic phenotype. In addition, aGVHD mice deprived from tyrosine displayed worse manifestations than the vehicle diet group. Interpretation The results demonstrated the roles and mechanisms of gut microbiota, indispensable metabolites and tyrosine in the progression of aGVHD, which can be an underlying biomarker for aGVHD diagnosis and treatment. Funding This research was funded by the International Cooperation and Exchange Program (81520108002), the National Key R&D Program of China, Stem Cell and Translation Research (2018YFA0109300), National Natural Science Foundation of China (81670169, 81670148, 81870080 and 91949115) and Natural Science Foundation of Zhejiang Province (LQ19H080006).

show abstract

“…The abundance of the intestinal microbiota is presented as total operational taxonomic units (OTUs), the specific data are shown in Table S8, Figure S8. (Tao et al, 2019). At the phylum level, nearly 97% of the total bacteria belonged to 10 phylum, and the rest were allocated to other unclassified bacteria.…”

Section: Composition Of Gut Microbial Communitymentioning

confidence: 98%

Naturally Occurring TPE-CA Maintains Gut Microbiota and Bile Acids Homeostasis via FXR Signaling Modulation of the Liver–Gut Axis

Liu

et al. 2020

Front. Pharmacol.

View full text Add to dashboard Cite

Antibiotics-induced changes in intestinal flora (dysbiosis) may have various effects on the host. Dysbiosis is associated with numerous metabolites including bile acids, which are produced in the liver from cholesterol and metabolized in the gut by intestinal microbiota. Total phenolic extracts of Citrus aurantium L. (TPE-CA) are rich in dietary flavanones and their glycosyl derivatives, including flavones, flavonols, polymethoxyflavones and coumarins, which exert positive health effects on the microbiota. The aim of this study is to elucidate the interplays between the intestinal microbiota and bile acids metabolism attributed to antibiotics. Mice were exposed to broad-spectrum antibiotics, such as ampicillin, streptomycin and clindamycin, for 14 days. This exposure resulted in reduced bacterial diversity and richness, and destroyed intestinal permeability. The homeostasis of bile acids was also affected. Subsequent TPE-CA administration, counteracted most of the dysbiosis, and reshaped intestinal permeability, these effects occurred via upregulation of zonula occludens 1 and occludin associated proteins and downregulation of serum endotoxin compared to the antibiotics group. TPE-CA maintained the homeostasis of bile acids via modulation of the liver-gut axis related farnesoid X receptor (FXR)/fibroblast growth factor 15 (FGF15) pathway and FXR-targeted protein. Our findings indicated that TPE-CA exerted a protective effect on the restoration of intestinal microbiota composition, reshaped barrier integrity and maintained bile acid homeostasis via the liver-gut axis with antibiotics-induced dysbiosis.

show abstract

Bacterial community mapping of the intestinal tract in acute pancreatitis rats based on 16S rDNA gene sequence analysis

Abstract: Numerous studies have revealed that the status of intestinal microbiota has a marked impact on inflammation, which may progressively aggravate the systemic inflammatory response caused by acute pancreatitis (AP).

Cited by 15 publications

References 40 publications

Application of biodegradable polymers as carbon sources in ex situ biofloc systems: Water quality and shift of microbial community

Application of biodegradable polymers as carbon sources in ex situ biofloc systems: Water quality and shift of microbial community

Tyrosine supplement ameliorates murine aGVHD by modulation of gut microbiome and metabolome

Naturally Occurring TPE-CA Maintains Gut Microbiota and Bile Acids Homeostasis via FXR Signaling Modulation of the Liver–Gut Axis

Contact Info

Product

Resources

About