Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge

Lozada, Mariana; Itria, Raúl Fabio; Figuerola, Eva Lucia Margarita; Babay, Paola Alejandra; Gettar, Raquel T.; Tullio, Luis A. de; Erijman, Leonardo

doi:10.1016/j.watres.2004.01.032

Cited by 53 publications

(42 citation statements)

References 33 publications

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“…Eight NPEO-degrading bacteria were isolated using an improved isolation method (Tamaki et al, 2005) with a PE03 medium containing 50 mg/L NP av9 EO as the sole added carbon source. The isolated NPEOdegrading bacteria belong to α-, β-, γ-Proteobacteria and Firmicutes Phylum (Fig.1), and the members of β-and α-Proteobacteria were dominated, which is consistent with the results obtained by culture independent methods from an NP 10 EO-amended sludge reactor (Lozada et al 2004(Lozada et al , 2006.…”

supporting

confidence: 86%

Degradation behaviors of nonylphenol ethoxylates by isolated bacteria using improved isolation method

Gu¹,

Zhang²,

Zhang³

et al. 2008

Journal of Environmental Sciences

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Nonylphenol ethoxylate (NPEO)-degrading bacteria were isolated from activated sludge using an improved isolation method, and the corresponding degradation behaviours were investigated. Eight NPEO-degrading strains distributed in genera Pseudomonas, Sphingomonas, Sphingobium, Cupriavidus, Ralstonia, Achromobacter, and Staphylococcus were acquired. The latter five genera have never been reported for the degradation of NPEOs. Four degradation patterns were observed for the eight pure strains. In pattern A, NPEOs were converted to short-chain NPEOs and carboxylated products, while in pattern B, lower ethoxylated oligomers appeared. Nonylphenol monoethoxylate was the main product in pattern C, while in pattern D ethoxylated units was oxidized but not shortened. Pattern C and D have not yet been reported.

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supporting

confidence: 86%

Degradation behaviors of nonylphenol ethoxylates by isolated bacteria using improved isolation method

Gu¹,

Zhang²,

Zhang³

et al. 2008

Journal of Environmental Sciences

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“…Proteobacteria have been observed to be dominant in activated sludge communities (Manz et al, 1994). Lozada et al (2004) have found that the beta subclass of Proteobacteria constitutes the predominant group of bacteria in NPEO10-amended activated sludge. On the other hand, most of the isolated NPEO-degrading bacteria, such as Acinetobacter, Aeromonas, Shewanella, Proteus, and Pseudomonas groups (Barberio et al, 2001;John and White, 1998), belong to γ-Proteobacteria.…”

Section: Dgge Analysis Of Bacterial Community Structuresmentioning

confidence: 94%

“…However, MLSS in the aerobic system decreased quickly from 2.3 to 1.5 g/L after NPEOs spiked into the influent, resulting in the increase of SVI from 95 to 167 ml/g. This phenomenon was attributed to the structural change of activated sludge flocs caused by the influence of the surfactant (Lozada et al, 2004). However, the SVI in the aerobic reactor decreased gradually to a relatively constant value of 119 ± 6.5 ml/g two weeks later.…”

Section: Reactors Performancementioning

confidence: 97%

Biotransformation of nonylphenol ethoxylates during sewage treatment under anaerobic and aerobic conditions

Zhang

Yang

Chen

2008

Journal of Environmental Sciences

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Biotransformation of nonylphenol ethoxylates (NPEOs) during continuous anaerobic sewage treatment was compared with the aerobic treatment of sewage spiked with 23 µmol/L technical NPEOs over a period of 90 d. Immediate degradation of NPEOs was observed under both anaerobic and aerobic conditions, indicating that the enzymes and bacteria required for NPEO degradation existed abundantly in both aerobic and anaerobic sludge. Both treatments achieved high removal (> 92%) of the spiked NPEO9 mixture. Liquid chromatography-mass spectrometry (LC-MS) analysis showed that short-chain NPEOs (NPEO1-NPEO3) accumulated in anaerobic (2.01-2.56 µmol/L) and aerobic (1.62-2.03 µmol/L) effluents, with nonylphenol (NP) (0.24-0.31 µmol/L) as another group of metabolites in the anaerobic effluent, and nonylphenoxy carboxylates (NPECs) (2.79-3.30 µmol/L) in the aerobic effluent. Significant accumulation of NP in the anaerobic sludge and NPEO1-3 in the sludge of two reactors was observed. These results indicated that it was difficult to control these harmful metabolites in the conventional treatment processes. Denaturing gradient gel electrophoresis profiles of sludge samples support the speculation that the NPEO degradation bacteria might be the dominant indigenous species.

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“…Many NPEO-degrading bacteria species belonging to the gamma subclass of the Proteobacteria have been isolated by culturedependent methods [35][36][37]. However, Lozada et al [38,39] found that a high proportion of members of Betaproteobacteria constitute the predominant group of bacteria in NPEO-fed microcosms using dot-blot hybridization and fluorescent in situ hybridization. This finding together with our result suggests that members of Betaproteobacteria might play an important role in NPEO degradation in the natural environment, which is quite different from those obtained using the culture-dependent methods.…”

Section: Changes Of Microbial Community Structures By T-rflpmentioning

confidence: 99%

Changes of catabolic genes and microbial community structures during biodegradation of nonylphenol ethoxylates and nonylphenol in natural water microcosms

Sei

Toyama

Ike

et al. 2008

Biochemical Engineering Journal

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Changes of possible key catabolic genes and microbial community structures during the degradation of NPEOs and NP in natural water microcosms were investigated using the most-probable-number-polymerase chain reaction (MPN-PCR) and terminal restriction fragment length polymorphism (T-RFLP). The copy number of catechol 2,3-dioxygenase (C23O) DNA increased significantly during NPEO and NP degradation, suggesting that meta-cleavage of the aromatic rings of NPEOs and NP might have happened. Catechol 1,2-dioxygenase (C12O) DNA, alkanecatabolic genes (alk), and 16S rDNA, on the other hand, did not change notably, suggesting that the two genes might not be the relevant genes for NPEOs and NP degradation. The 16S rRNA gene-based T-RFLP analysis results indicated that specific and different dominant (or degrading) bacteria should be selected, depending on the substances. A strain with a DNA length of 78 bp, which might be affiliated with the beta subclass of Proteobacteria, became the dominant species for NPEO degradation, while strains at 88 and 198 bp were dominant in the NP microcosm. Diversity of microbial community structure tended to be simplified after NPEO degradation, while that in the NP microcosm remained relatively stable. Five clusters were obtained according to the similarity in community structures of different microcosms by cluster analysis, which were consistent with the biodegradation behaviors of different microcosms. This is the first report on genetic evidence of a possible aromatic ring meta-cleaving pathway of NPEOs and NP in an aquatic environment.

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Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge

Cited by 53 publications

References 33 publications

Degradation behaviors of nonylphenol ethoxylates by isolated bacteria using improved isolation method

Degradation behaviors of nonylphenol ethoxylates by isolated bacteria using improved isolation method

Biotransformation of nonylphenol ethoxylates during sewage treatment under anaerobic and aerobic conditions

Changes of catabolic genes and microbial community structures during biodegradation of nonylphenol ethoxylates and nonylphenol in natural water microcosms

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