Bacterial composition and community structure of the oropharynx of adults with asthma are associated with environmental factors

Zhao, Hongcheng; Liu, Jia; Zhu, Jingyuan; Yang, Fan; Wu, Huiying; Ba, Yue; Cui, Liuxin; Chen, Ruiying; Chen, Shuaiyin

doi:10.1016/j.micpath.2020.104505

Cited by 8 publications

(4 citation statements)

References 66 publications

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“…Cupriavidus acts as an opportunistic pathogen in soil, and its association with asthma has been shown in previous studies. Zhao et al (2020) showed that the abundance of Cupriavidus increased significantly in the URT of patients with asthma that were exposed to high concentrations of PM2.5 particulates. In the acute inflammatory phase, the bacteria with high abundance in the LRT and URT were similar, and only Cupriavidus was more abundant at the airway remodeling stage in the LRT.…”

Section: Discussionmentioning

confidence: 99%

Respiratory Microbiota Profiles Associated With the Progression From Airway Inflammation to Remodeling in Mice With OVA-Induced Asthma

Zheng

et al. 2021

Front. Microbiol.

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BackgroundThe dysbiosis of respiratory microbiota plays an important role in asthma development. However, there is limited information on the changes in the respiratory microbiota and how these affect the host during the progression from acute allergic inflammation to airway remodeling in asthma.ObjectiveAn ovalbumin (OVA)-induced mouse model of chronic asthma was established to explore the dynamic changes in the respiratory microbiota in the different stages of asthma and their association with chronic asthma progression.MethodsHematoxylin and eosin (H&E), periodic acid-schiff (PAS), and Masson staining were performed to observe the pathological changes in the lung tissues of asthmatic mice. The respiratory microbiota was analyzed using 16S rRNA gene sequencing followed by taxonomical analysis. The cytokine levels in bronchoalveolar lavage fluid (BALF) specimens were measured. The matrix metallopeptidase 9 (MMP-9) and vascular endothelial growth factor (VEGF-A) expression levels in lung tissues were measured to detect airway remodeling in OVA-challenged mice.ResultsAcute allergic inflammation was the major manifestation at weeks 1 and 2 after OVA atomization stimulation, whereas at week 6 after the stimulation, airway remodeling was the most prominent observation. In the acute inflammatory stage, Pseudomonas was more abundant, whereas Staphylococcus and Cupriavidus were more abundant at the airway remodeling stage. The microbial compositions of the upper and lower respiratory tracts were similar. However, the dominant respiratory microbiota in the acute inflammatory and airway remodeling phases were different. Metagenomic functional prediction showed that the pathways significantly upregulated in the acute inflammatory phase and airway remodeling phase were different. The cytokine levels in BALF and the expression patterns of proteins associated with airway remodeling in the lung tissue were consistent with the metagenomic function results.ConclusionThe dynamic changes in respiratory microbiota are closely associated with the progression of chronic asthma. Metagenomic functional prediction indicated the changes associated with acute allergic inflammation and airway remodeling.

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Section: Discussionmentioning

confidence: 99%

Respiratory Microbiota Profiles Associated With the Progression From Airway Inflammation to Remodeling in Mice With OVA-Induced Asthma

Zheng

et al. 2021

Front. Microbiol.

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“…30 In addition to the complexity of associations between humans and these microorganisms, research carried out in this field has also emphasised their essential role in preserving human health. 31 Over recent years, a number of studies have focused on the utility of metagenomics for deciphering the underlying mechanisms in allergic diseases, with intriguing data available for atopic dermatitis, 32 allergic rhinitis, 33 asthma, 34,35 and food allergy, 36 16S rRNA sequencing consists in the amplification of the hypervariable regions of the 16S ribosomal RNA subunit genes, which are unique for specific bacterial genera. Quality control evaluation of sequencing reads is similar to these already described.…”

Section: Genomics and Metagenomicsmentioning

confidence: 99%

“… 30 In addition to the complexity of associations between humans and these microorganisms, research carried out in this field has also emphasised their essential role in preserving human health. 31 Over recent years, a number of studies have focused on the utility of metagenomics for deciphering the underlying mechanisms in allergic diseases, with intriguing data available for atopic dermatitis, 32 allergic rhinitis, 33 asthma, 34 , 35 and food allergy, 36 among others. The main high‐throughput sequencing technologies used in microbiota characterisation include PCR amplicon‐based sequencing (16S and 18S rRNA and internal transcribed spacer, ITS, sequencing), DNA‐based shotgun metagenomic sequencing, RNA‐based metatranscriptomic sequencing, and virome sequencing.…”

Section: Basic Omics Strategiesmentioning

confidence: 99%

Omics technologies in allergy and asthma research: An EAACI position paper

Radzikowska

Baerenfaller

Cornejo-García

et al. 2022

Allergy

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Allergic diseases and asthma are heterogenous chronic inflammatory conditions with several distinct complex endotypes. Both environmental and genetic factors can influence the development and progression of allergy. Complex pathogenetic pathways observed in allergic disorders present a challenge in patient management and successful targeted treatment strategies. The increasing availability of high-throughput omics technologies, such as genomics, epigenomics, transcriptomics, proteomics, and metabolomics allows studying biochemical systems and pathophysiological processes underlying allergic responses. Additionally, omics techniques present clinical These different cellular components are tightly interconnected with TA B L E 1 Summary of methods, protocols, and data analysis pipelines most frequently used in omics approaches for allergic diseases research Methods and protocols Data analysis pipeline Mechanisms of allergic diseases Genomics and transcriptomics Microarray 181,182GWAS 183 and RNA microarray bioinformatics 184,185

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“…Asthma has largely been studied on its relationship with microbiome. Several studies have identified, on one hand, the respiratory microbiota pattern related to asthma (high abundance of Haemophilus influenzae, Streptococcus pneumoniae, Staphylococcus aureus , and Moraxella catarrhalis , which can potentially play a pathogenic role) ( 87 , 92 ) and on the other, that these species have a role on the inflammatory response which could determine the outcomes in asthma ( 93 ).…”

Section: Nasopharyngeal Microbiome and Respiratory Diseasementioning

confidence: 99%

Related Factors to Streptococcus pneumoniae Invasive Infection and Clinical Manifestations: The Potential Role of Nasopharyngeal Microbiome

et al. 2021

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Infections of the lower respiratory tract, such as pneumonia, are one of the leading causes of death worldwide. Streptococcus pneumoniae might colonize the upper respiratory tract and is the main aetiological agent of community-acquired pneumonia (CAP). In the last decades, several factors related to the host, the microorganism and the antibiotic therapy have been investigated to identify risk factors associated with the development of invasive pneumococcal disease (IPD). Nevertheless, these factors themselves do not explain the risk of developing disease or its severity. Recently, some studies have focused on the importance of nasopharyngeal (NP) microbiome and its relation to respiratory health. This review presents existing evidence of the potential role of NP microbiome in the development of IPD.

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Bacterial composition and community structure of the oropharynx of adults with asthma are associated with environmental factors

Cited by 8 publications

References 66 publications

Respiratory Microbiota Profiles Associated With the Progression From Airway Inflammation to Remodeling in Mice With OVA-Induced Asthma

Respiratory Microbiota Profiles Associated With the Progression From Airway Inflammation to Remodeling in Mice With OVA-Induced Asthma

Omics technologies in allergy and asthma research: An EAACI position paper

Related Factors to Streptococcus pneumoniae Invasive Infection and Clinical Manifestations: The Potential Role of Nasopharyngeal Microbiome

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