Bacterial concentration and detection using an ultrasonic nanosieve within a microfluidic device

Ang, Bryan; Habibi, Ruhollah; Kett, Ciaren; Chin, Wai Hoe; Barr, Jeremy J.; Tuck, Kellie L.; Neild, Adrian; Cadarso, Víctor J.

doi:10.1016/j.snb.2022.132769

Cited by 17 publications

(10 citation statements)

References 46 publications

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“…This is likely due to presence of milk fat globules in milk, which share a similar size range (0.1 to 10 μm) to the bacteria (0.5 to 2 μm), which reduces the number of attachment sites for bacteria and therefore capturing efficiency. The system allowed for >80% capture efficiency at 10 μL/min, a 33-fold increase in flow rate when compared to the previous version of the device, which was limited to a low flow rate of 0.3 μL/min and small initial sample volumes of ≤10 μL . The larger flow rate performance afforded by the device enables mL-scale sample volume processing required from quality control standards which was not possible with prior versions of SWANS. − For lower complexity matrices, the flow rate performance is doubled, with the buffer sample maintaining >80% at 20 μL/min (see Figure b).…”

Section: Resultsmentioning

confidence: 99%

“…In this work, we designed and fabricated an upscaled system enabling the capturing of bacterial cells within milk, a highly complex biological medium, which was not possible in previous systems due to their limited flow rates. ,, This larger system enabled a 33-fold increase (up to 10 μL/min) in flow rate than the most recently reported SWANS system while retaining high efficiency (>80%) bacteria capture in a complex fluid. Furthermore, our device allows the direct staining and fluorescent detection of the captured cells, within just 3 min of completion of capturing, enabling extremely fast detection and quantification of bacteria without requiring any additional steps.…”

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confidence: 99%

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Rapid Concentration and Detection of Bacteria in Milk Using a Microfluidic Surface Acoustic Wave Activated Nanosieve

Ang,

Jirapanjawat,

Tay

et al. 2024

ACS Sens.

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Rapid detection of microbes is a key feature for monitoring food quality. Unfortunately, current detection systems rely on labor-intensive and time-consuming lab-based processes that are not suitable for point-of-interest applications and typically require several days before results are available. Here, we demonstrate a microfluidic system capable of rapidly concentrating, fluorescent staining, and detecting bacteria in unprocessed complex biological media such as milk. This concentration is done using a surface acoustic wave-driven microfluidic device which operates based on the Bjerknes force, a force generated on one particle by another in its close proximity. We exploit this effect by exciting a tightly packed bed of 50 μm polystyrene microparticles temporarily with surface acoustic waves within a microfluidic device to capture and release bacterial cells on demand. The bacterial cells are fluorescently stained during capture and then detected using fluorescence microscopy upon release. This device offers a high capturing efficiency (>80%) and a 34 Colony Forming Units (CFU)/mL limit of detection, which is 1 order of magnitude below that of plate counting at 30 CFU per standard 100 μL plate (or 300 CFU/mL). This can be attained in just 1 h of processing at 10 μL/min. With this system, we demonstrate that bacterial detection from extremely low concentration samples down to the order of ∼10 CFU/mL is possible without requiring any additional external pre-or postprocessing.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Rapid Concentration and Detection of Bacteria in Milk Using a Microfluidic Surface Acoustic Wave Activated Nanosieve

Ang,

Jirapanjawat,

Tay

et al. 2024

ACS Sens.

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“…Thus, active separation techniques are highly flexible and can precisely control particles as needed. This also implies that active separation methods are well-suited for submicron particles and nanoparticles, including the separation of exosomes, 22,86,87 bacteria, [88][89][90] viruses, 34,91 and so forth. [92][93][94][95] But external force fields may cause damage to the activity of biological particles.…”

Section: Active Separation Methodsmentioning

confidence: 99%

Biological particle separation techniques based on microfluidics

Wang,

Xu,

Cai

et al. 2024

Interdisciplinary Medicine

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Biological particle separation has wide applications in medical diagnosis, bioengineering, and various other domains. Traditional methods, such as filtration, density gradient centrifugation, and size exclusion chromatography, face many challenges, including low separation resolution, low purity, and the inability to be seamlessly integrated into continuous processes. The development of microfluidics has paved the way for efficient and precise biological particle separation. Microfluidic chip‐based methods can generally be performed continuously and automatically, and microfluidic chips can integrate multilevel operations, including mixing, separation, detection, and so forth, thereby achieving continuous processing of particles at various levels. This review comprehensively investigates biological particle separation techniques based on microfluidic chips. According to the different sources of force effect on the particles during the separation process, they can be divided into active separation, passive separation, and affinity separation. We introduce the principles and device design of these methods respectively, and compare their advantages and disadvantages. For the introduction of each method, we used the most classic and latest research cases as much as possible. Additionally, we discussed the differences between experimental standard particles and biological particles. Finally, we summarized the current limitations and challenges of existing microfluidic separation techniques, while exploring future trends and prospects.

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“…In a separate application, the work could also be used to reduce detection times of acoustic sensors. For example, recent work 58 that utilised ultrasonics for the capture and fluorescence detection of Escherichia coli bacterial cells within low concentration samples (<10 3 CFU mL −1 , CFU: colony forming units) was limited to a flow rate of 0.3 μL min −1 and required 32 min of ultrasonic concentration to detect bacterial concentrations down to ∼10 2 CFU mL −1 . The glass insert microchannel adaptation from this work, combined with the large potential for upscaling of this system, would allow for rapid bacterial detection within only a few minutes, creating an avenue for mL-scale processing required for food and water quality control which otherwise, could not be done before within an industrially practical timeframe due to their limited flow rate capability.…”

Section: Power and Flow Rate Comparisonmentioning

confidence: 99%

Glass-embedded PDMS microfluidic device for enhanced concentration of nanoparticles using an ultrasonic nanosieve

et al. 2023

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Bacterial concentration and detection using an ultrasonic nanosieve within a microfluidic device

Cited by 17 publications

References 46 publications

Rapid Concentration and Detection of Bacteria in Milk Using a Microfluidic Surface Acoustic Wave Activated Nanosieve

Rapid Concentration and Detection of Bacteria in Milk Using a Microfluidic Surface Acoustic Wave Activated Nanosieve

Biological particle separation techniques based on microfluidics

Glass-embedded PDMS microfluidic device for enhanced concentration of nanoparticles using an ultrasonic nanosieve

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