Bacterial Consortium and Axenic Cultures Isolated from Activated Sewage Sludge for Biodegradation of Imidazolium-based Ionic Liquid

Abstract

Background: Understanding the effects of pretreatment on anaerobic digestion of sludge waste from wastewater treatment plants is becoming increasingly important, as impetus moves towards the utilization of sludge for renewable energy production. Although the field of sludge pretreatment has progressed significantly over the past decade, critical questions concerning the underlying microbial interactions remain unanswered. In this study, a metagenomic approach was adopted to investigate the microbial composition and gene content contributing to enhanced biogas production from sludge subjected to a novel pretreatment method (maintaining pH at 10 for 8 days) compared to other documented methods (ultrasonic, thermal and thermal-alkaline).

Genome‐centric resolution of microbial diversity, metabolism and interactions in anaerobic digestion

“…Early metagenomic studies relied largely on genecentric analyses (Venter et al, 2004; with the recovery of individual genomes limited to environments dominated by few distinct populations (Tyson et al, 2004). These gene-centric approaches are biased towards existing databases, hereby overlooking a significant fraction of the novel diversity (Jaenicke et al, 2010;Wong et al, 2013). In addition, as only an overview of the metabolic potential of the community is provided without assigning functions to individual populations, important metabolic interactions may remain undetected.…”

“…This process is a critical component of the global carbon cycle as well as industrially relevant as a waste management strategy and for the production of bioenergy (Amani et al, 2010). Due to the complexity of the communities involved, anaerobic digesters (ADs) remain genomically underexplored and most metagenomic studies have relied on gene-centric approaches (Jaenicke et al, 2010;Hanreich et al, 2013;Wong et al, 2013;Solli et al, 2014;Stolze et al, 2015). The recovery of population genomes from various engineered systems has provided genomic insight into candidate phyla such as TM7 (Albertsen et al, 2013) and KSB3 (Sekiguchi et al, 2015), which is responsible for filamentous bulking in anaerobic wastewater treatment, and microbial interactions such as synergistic networks within terephthalatedegrading bioreactors (Nobu et al, 2014).…”

Impact of nanoscale zerovalent iron on volatile fatty acid production from food waste: key enzymes and microbial community

“…In X3 samples, Bacteroidetes decreased and another dominant phylum, Actinobacteria (16.07%), which degrade and produce acid during anaerobic digestion with organic matter, appeared; there is a close relationship. 33 Bacteroidetes was also a dominant bacterium especially in the process of treating cow dung with a continuously stirred tank reactor (CSTR) anaerobic digestion system, mixing methane with cow dung and straw, and anaerobic digestion of some organic wastes rich in sugars, such as food waste. 34 In this study, the abundance of Bacteroidetes in X2 and X3 samples was reduced, possibly because X2 and X3 were sampled at 48 h of fermentation, and the methanation inhibition treatment was carried out, resulting in a decrease in abundance.…”

Is understanding the structure-function of microbial community of sludge suitable using DNA-based molecular methods?

“…However, this process produced vast quantities of highly organic waste activated sludge, and this by-product mass continues to increase with the expansion of population and industry (Cui and Jahng, 2006;Zhang et al, 2010). Sludge disposal by landfill or incineration may no longer be feasible in the near future due to land scarcity, high waste charge and increasing stringent environmental control regulations (Weemaes et al, 2000;Wong et al, 2013;Wang et al, 2018). Therefore, the strategy for sludge management is shifting towards its reutilization as a potential source for renewable energy (Mossakowska et al, 1998;Rulkens, 2007).…”