Bacterial degradation of polyester polyurethane

Kay, M.J.; Morton, L.H.G.; Prince, E.L.

doi:10.1016/0265-3036(91)90012-g

Cited by 108 publications

(56 citation statements)

References 5 publications

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“…Most studies on the microbial colonization and biodeterioration of plastics have focused on polyurethanes (Barratt et al, 2003;Bentham et al, 1987;Kay et al, 1991), bacterial polyesters (Mergaert et al, 1996) and nylon (Deguchi et al, 1997). By contrast, there have been fewer studies focusing on the colonization of pPVC in situ (Upsher, 1984;Upsher & Roseblade, 1984;Webb et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Fungal colonization of soil-buried plasticized polyvinyl chloride (pPVC) and the impact of incorporated biocides

2006

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Plasticized polyvinyl chloride (pPVC) with or without incorporated biocides was buried in grassland and forest soil for up to 10 months. The change with time in viable counts of fungi on the plastic surface was followed, together with the percentage capable of clearing the two plasticizers dioctyl adipate (DOA) and dioctyl phthalate (DOP). With time fungal total viable counts (TVC) on control pPVC increased and the fraction able to clear DOA was considerably higher than the average estimated in both soil types. A total of 92 fungal morphotypes were isolated from grassland soil and 42 from forest soil with the greatest variety of fungal isolates observed on control pPVC. The incorporation of biocides into pPVC affected both fungal TVC and the richness of species isolated. The biocides NCMP [n-(trichloromethylthio)phthalimide], OBPA (10,109-oxybisphenoxarsine) and OIT (2-n-octyl-4-isothiazolin-3-one) were the most effective in grassland soil, and TCMP [2,3,5,6-tetrachloro-4-(methylsulphonyl)pyridine] and NCMP the most effective in forest soil. In grassland soil, Penicillium janthinellum established as a principal colonizer and was recovered from all pPVC types. DOP clearers were found at much lower levels than DOA clearers, with Doratomyces spp. being the most efficient. At the end of 10 months the physical properties of the pPVC were altered; changes in stiffness were the most significant for heavily colonized grassland-buried pPVC samples, whereas in forest soil, the extensibility of the pPVC was affected more than the stiffness. These results suggest that fungi are important colonizers of pPVC buried in soil and that enrichment of soil fungi capable of clearing DOA occurs during colonization of the plastic surface. The results also demonstrate that incorporated biocides have a marked impact on the richness of species colonizing the pPVC surface.

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Section: Introductionmentioning

confidence: 99%

Fungal colonization of soil-buried plasticized polyvinyl chloride (pPVC) and the impact of incorporated biocides

2006

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“…A wide variety of fungi and Grampositive and Gram-negative bacteria have been shown to secrete enzymes capable of degrading PUs. Among bacteria, some of the most well-studied PU-degrading organisms are pseudomonadsGram-negative, heterotrophic bacteria (2,(4)(5)(6)(7)(8). Several Pseudomonas species including Pseudomonas chlororaphis, Pseudomonas aeruginosa, and Pseudomonas fluorescens have been shown to degrade a model polyester PU, Impranil DLN (Impranil) (4)(5)(6).…”

mentioning

confidence: 99%

“…Among bacteria, some of the most well-studied PU-degrading organisms are pseudomonadsGram-negative, heterotrophic bacteria (2,(4)(5)(6)(7)(8). Several Pseudomonas species including Pseudomonas chlororaphis, Pseudomonas aeruginosa, and Pseudomonas fluorescens have been shown to degrade a model polyester PU, Impranil DLN (Impranil) (4)(5)(6). Three PU-degrading enzymes have been purified and characterized from some of these strains, including polyurethane esterases A (PueA) and B (PueB) from Pseudomonas chlororaphis (5,9) and polyurethane lipase (PulA) from P. fluorescens (5,(9)(10)(11).…”

mentioning

confidence: 99%

Carbon Catabolite Repression and Impranil Polyurethane Degradation in Pseudomonas protegens Strain Pf-5

Hung

Zingarelli

Nadeau

et al. 2016

Appl Environ Microbiol

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Polyester polyurethane (PU) coatings are widely used to help protect underlying structural surfaces but are susceptible to biological degradation. PUs are susceptible to degradation by Pseudomonas species, due in part to the degradative activity of secreted hydrolytic enzymes. Microorganisms often respond to environmental cues by secreting enzymes or secondary metabolites to benefit their survival. This study investigated the impact of exposing several Pseudomonas strains to select carbon sources on the degradation of the colloidal polyester polyurethane Impranil DLN (Impranil). The prototypic Pseudomonas protegens strain Pf-5 exhibited Impranil-degrading activities when grown in sodium citrate but not in glucose-containing medium. Glucose also inhibited the induction of Impranil-degrading activity by citrate-fed Pf-5 in a dose-dependent manner. Biochemical and mutational analyses identified two extracellular lipases present in the Pf-5 culture supernatant (PueA and PueB) that were involved in degradation of Impranil. Deletion of the pueA gene reduced Impranil-clearing activities, while pueB deletion exhibited little effect. Removal of both genes was necessary to stop degradation of the polyurethane. Bioinformatic analysis showed that putative Cbr/Hfq/Crc-mediated regulatory elements were present in the intergenic sequences upstream of both pueA and pueB genes. Our results confirmed that both PueA and PueB extracellular enzymes act in concert to degrade Impranil. Furthermore, our data showed that carbon sources in the growth medium directly affected the levels of Impranil-degrading activity but that carbon source effects varied among Pseudomonas strains. This study uncovered an intricate and complicated regulation of P. protegens PU degradation activity controlled by carbon catabolite repression. IMPORTANCEPolyurethane (PU) coatings are commonly used to protect metals from corrosion. Microbiologically induced PU degradation might pose a substantial problem for the integrity of these coatings. Microorganisms from diverse genera, including pseudomonads, possess the ability to degrade PUs via various means. This work identified two extracellular lipases, PueA and PueB, secreted by P. protegens strain Pf-5, to be responsible for the degradation of a colloidal polyester PU, Impranil. This study also revealed that the expression of the degradative activity by strain Pf-5 is controlled by glucose carbon catabolite repression. Furthermore, this study showed that the Impranil-degrading activity of many other Pseudomonas strains could be influenced by different carbon sources. This work shed light on the carbon source regulation of PU degradation activity among pseudomonads and identified the polyurethane lipases in P. protegens.

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“…It has been also reported that synthetic polyester-type polyurethane foams are decomposed by a number of fungi and bacteria (Daby and Kaplan 1968;Crabbe et al 1994;Nakajima-Kambe et al 1995), and this degradation is generally initiated by hydrolysis of the ester bond with hydrolytic enzymes such as esterase. Many groups reported the purification and characterization of those enzymes (Pathirana and Seal 1984;Kay et al 1991). However, it is known that polyether-type polyurethane foam is difficult to degrade.…”

Section: Discussionmentioning

confidence: 99%

Biodegradability and Risk Assessment of Biomass-based Polymeric Materials

Han¹,

Park²,

Jang³

et al. 2015

Journal of Forest and Environmental Science

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With the intention to solve environmental problems caused by synthetic plastics from petroleum resources, biodegradable polyurethane foams and thermosetting moldings were prepared from biomass, such as wood and wheat bran by liquefaction method. Biodegradability of these biomass-based polymeric materials was investigated. In activated sludge, polyurethane foams from liquefied wheat bran and thermosetting molding from phenolated wood were decomposed approximately 14% and 29% for 20 days, respectively. One of the wood fungi, Coriolus versicolor was able to grow without supplemental nutrition, only with distilled water and polyurethane foam as a nutrition source. Risk assessments were also conducted and results showed that estrogenicity, mutagenicity, and carcinogenicity were not observed in the extractives of biomass-based polymeric materials.

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Bacterial degradation of polyester polyurethane

Cited by 108 publications

References 5 publications

Fungal colonization of soil-buried plasticized polyvinyl chloride (pPVC) and the impact of incorporated biocides

Fungal colonization of soil-buried plasticized polyvinyl chloride (pPVC) and the impact of incorporated biocides

Carbon Catabolite Repression and Impranil Polyurethane Degradation in Pseudomonas protegens Strain Pf-5

Biodegradability and Risk Assessment of Biomass-based Polymeric Materials

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