Bacterial Degradation of Some Organophosphate Compounds

Kumar, Deepak; Bhatia, Divya; Rathi, Meenu

doi:10.1007/978-81-322-1801-2_48

Cited by 4 publications

(2 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several bacterial species, such as Pseudomonas sp. and Bacillus sp., have been reported to degrade a wide range of organophosphorus insecticides, including FNT. − However, there have been only a few studies of fungal-associated degradation of FNT . Unlike prokaryotes, eukaryotic fungi have demonstrated diverse metabolic potential, resulting in metabolites similar to those produced in the mammalian enzyme system, − which would help us reveal the metabolic fates of organic compounds occurring in mammalian liver cells.…”

Section: Introductionmentioning

confidence: 99%

Metabolism of an Insecticide Fenitrothion by Cunninghamella elegans ATCC36112

Zhu

Jeong

et al. 2017

J. Agric. Food Chem.

View full text Add to dashboard Cite

In this study, the detailed metabolic pathways of fenitrothion (FNT), an organophosphorus insecticide by Cunninghamella elegans, were investigated. Approximately 81% of FNT was degraded within 5 days after treatment with concomitant accumulation of four metabolites (M1-M4). The four metabolites were separated by high-performance liquid chromatography, and their structures were identified by mass spectroscopy and/or nuclear magnetic resonance. M3 is confirmed to be an initial precursor of others and identified as fenitrothion-oxon. On the basis of their metabolic profiling, the possible metabolic pathways involved in phase I and II metabolism of FNT by C. elegans was proposed. We also found that C. elegans was able to efficiently and rapidly degrade other organophosphorus pesticides (OPs). Thus, these results will provide insight into understanding of the fungal degradation of FNT and the potential application for bioremediation of OPs. Furthermore, the ability of C. elegans to mimic mammalian metabolism would help us elucidate the metabolic fates of organic compounds occurring in mammalian liver cells and evaluate their toxicity and potential adverse effects.

show abstract

Section: Introductionmentioning

confidence: 99%

Metabolism of an Insecticide Fenitrothion by Cunninghamella elegans ATCC36112

Zhu

Jeong

et al. 2017

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Carbon nanotubes are also a vector for antibiotics and insulin although questions have been raised about the ecotoxicological effects and life cycle issues of nanomaterial release into natural ecosystems (e.g., refs , ). One candidate for inclusion in nanotube remediation systems is phosphatase, which has broad substrate specificity and can degrade organophosphate contaminants, such as pesticides − and nerve agents . These enzymes have been well studied in coastal aquatic ecosystems because of their potential importance in controlling phosphorus cycles…”

Section: Introductionmentioning

confidence: 99%

Eco-friendly Organic Nanotubes Encapsulating Alkaline Phosphatase and Ecotoxicology of Nanotubes to Natural Bacterial Assemblages in Coastal Estuarine Waters

Montgomery¹,

Collins²,

Boyd³

et al. 2019

ACS Omega

View full text Add to dashboard Cite

Phosphatase-encapsulated nanotubes have potential in environmental remediation of organophosphate contaminants (e.g., pesticides, nerve agents). We investigated alkaline phosphatase (AP) activity when encapsulated in self-assembled lithocholic acid nanotubes (LCA-AP) in water samples along a transect from Cypress bog headwaters through estuarine waters and to Atlantic Ocean seawater. Apparent V max (app V max) for both LCA-AP and unencapsulated AP (Free-AP) was most rapid at mid-estuary and most inhibited at the humic-rich bog. LCA-AP retained a higher-activity percentage, suggesting that encapsulation may afford some protection from denaturing effects of humics. Apparent K m (app K m) of Free-AP (1–2.3 μM) was largely unaffected by preincubation with transect water, whereas app K m of LCA-AP was higher with bog water (5.3 μM) relative to other stations. When comparing Free-AP and LCA-AP, increasing salinity generally decreased the catalytic efficiency of the LCA-AP, but had little effect on that of the Free-AP. In addition, both showed the same pattern of lowest efficiency in bog water, which increased with salinity to 21 practical salinity units before decreasing at full-strength salinity. With the exception of the similarly low values in the bog water (1.04 for LCA-AP, 1.34 for Free-AP), absolute values of catalytic efficiency for LCA-AP were about 17% (range: 14.5–19.3%) of that for Free-AP. Nanotube addition had little ecotoxicological effect on heterotrophic bacterial production in waters sampled along the transect. Microbially associated, intrinsic AP activity showed a similar pattern along the transect to LCA-AP, suggesting that AP environmental control and regulation in nature may inform study of nanomaterials.

show abstract