Bacterial degradation of the herbicide pendimethalin and activity evaluation of its metabolites

Kole, Ramen Kumar; Saha, J. K.; Pal, S.; Chaudhuri, Surabhi; Chowdhury, A.

doi:10.1007/bf00195503

Cited by 30 publications

(35 citation statements)

References 2 publications

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“…But the pentane, released by oxidative N-dealkylation of pendimethalin, was utilized as the sole source of carbon and energy for the growth of the organism. The acetylation, aryl methyl oxidation and cyclization products of pendimethalin, as reported in Azotobacter chroococcum (Holding and Collee, 1971;Kole et al, 1994). Megadi et al (2010) reported that pendimethalin degradation with Bacillus circulans was by nitroreduction and oxidative N-dealkylation via secretion of pendimethalin nitroreductase and pendimethalin N-dealkylase.…”

Section: Dissipation Of Pendimethalin By P Putida (E15) In Mineral Lmentioning

confidence: 92%

“…For instance, Kole et al (1994) observed that 45 and 55% metabolism of pendimethalin caused by Azotobacter chroococcum after 10 and 20 days of incubation, respectively. Azotobacter vinelandii was isolated from a pendimethalin-treated barley rhizosphere.…”

Section: Dissipation Of Pendimethalin By P Putida (E15) In Mineral Lmentioning

confidence: 99%

“…The microbial degradation of dinitroaniline herbicides, pendimethalin and trifluralin has been repor-ted to occur most often by oxidative N-dealkylation and nitroreduction. The nitro group reduction and oxidative Ndealkylation destroys the herbicidal activity of pendimethalin, leading to its detoxification (Zayed et al, 1983;Singh and Kulashrestha, 1991;Kole et al, 1994;Megadi et al, 2010).…”

Section: Dissipation Of Pendimethalin By P Putida (E15) In Mineral Lmentioning

confidence: 99%

“…Soil microorganisms that are repeatedly exposed to pesticides may develop new capabilities to degrade such chemicals (Vidali, 2001). There are some reports on the degradation of pendimethalin by microorganisms comprising Azotobacter chroococcum, A. vinelandii and Bacillus circulans Singh and Kulashrestha, 1991;Kole et al, 1994;Megadi et al, 2010). The success of bioremediation depends not only on the high degradation ability but also on the stability of active microorganisms under varied conditions, such as changes in pH and temperature (Pattanasupong et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Bioremediation of pendimethalin-contaminated soil

Belal¹,

El-Sayed²,

El-Nady³

2013

Afr. J. Microbiol. Res.

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One strain of microorganisms was isolated from soil previously treated with pendimethalin using enrichment technique and identified using 16S rDNA as Pseudomonas putida (E15). The effect of pH and temperature on the growth ability of the tested strain was investigated. The results show that the optimum pH and temperature for the growth of pendimethalin dissipating strain were 7 and 30°C, respectively. P. putida was used to dissipate pendimethalin from mineral liquid medium with half-live of 5.46 days. Pendimethalin half-live was 51.9 days in untreated mineral liquid medium. P. putida and compost were also evaluated for detoxification of pendimethalin in clay soil. P. putida and compost were effective in pendimethalin dissipation in soil with half-live of 4.67 and 5 days, respectively. Pendimethalin half-live was 62.43 days in untreated soil. Pendimethalin treatment affected analysis of the microbial population growing in P. putida or compost treating soil leachates showed an overall increase in the population of microorganisms. There is no toxicity of pendimethalin detected in soil on cucumber plants after treatment with P. putida or compost. Pendimethalin significantly decreased germination and increased cucumber seedlings mortality rate. P. putida and compost treatments increased the growth parameters. Moreover, no significant difference was observed in the most growth parameters between P. putida and compost treatments. Abnormal development of xylem tissue was observed in pendimethalin contaminated soil as a result of phytotoxicity. The results suggest that bioremediation by P. putida and compost was considered to be effective method for detoxification of pendimethalin in soil.

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Section: Dissipation Of Pendimethalin By P Putida (E15) In Mineral Lmentioning

confidence: 92%

Section: Dissipation Of Pendimethalin By P Putida (E15) In Mineral Lmentioning

confidence: 99%

Section: Dissipation Of Pendimethalin By P Putida (E15) In Mineral Lmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bioremediation of pendimethalin-contaminated soil

Belal¹,

El-Sayed²,

El-Nady³

2013

Afr. J. Microbiol. Res.

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“…For instance, Kole et al (1994) observed that 45% and 55% metabolism of pendimethalin was occurred by Azotobacter chroococcum after 10 and 20 days of incubation, respectively. Azotobacter vinelandii was isolated from a pendimethalin-treated barley rhizosphere.…”

Section: Biodegradation Of Pendimethalin By P Resinovorans E20 In Aqmentioning

confidence: 99%

Degenotoxicity of Pendimethalin Contaminated Clay Soil by Pseudomonas resinovorans Using Anatomical, Cytogenetic and Biochemical Analysis in Vicia faba Plants

Aboulila¹,

Belal²,

Metwaly³

et al. 2016

Int. J. Curr. Res. Biosci. Plant Biol.

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A b s t r a c t A r t i c l e I n f oBiodegradation of the selective herbicide pendimethalin which is used to control most annual grasses was investigated in water and soil. A pendimethalin degrading bacterial isolate (designated strain E20) was isolated using enrichment technique from wheat soil previously treated with the herbicide pendimethalin on the basis of morphological, physiological and genetical (16s rDNA) tests, this bacterial isolate proved to be identified as Pseudomonas resinovorans E20. Results showed that the optimum pH and temperature for the growth of pendimethalin degrading strain were 7 and 30°C, respectively. Additional carbon sources (glucose, lactose, starch, carboxymethyl cellulose and phenol) decreased pendimethalin degradation. Pseudomonas resinovorans E20 was used to remove pendimethalin from aquatic system with half-life of 5.9 days. Pendimethalin halflife was 49.2 days in untreated mineral liquid medium as control. Pseudomonas resinovorans E20 was also evaluated for degradation of pendimethalin in clay soil with half-life of 5.004 days, its half-life was 66.9 days in untreated clay soil. The obtained results illustrated that pendimethalin treatment reduced all measured plant features (plant height, root length, xylem vessels diameter, thickness of phloem tissue, vascular bundle as well as vascular bundle width) in comparison to the other treatments. On the other side, treatment with Pseudomonas resinovorans E20 led to improving and increasing the above investigated parameters. Evaluation of the genotoxic effects of pendimethalin on the genetic material of Vicia faba plants revealed that, the highest abnormal mitosis percentage (63.28%) was recorded for pendimethalin treated plants. On the other hand, Vicia faba plants treated with the bacterial strain Pseudomonas resinovorans E20 decrease the abnormal mitosis percentage which reached to 21.35% in comparison with the control treatment which gave a percentage of 3.85%. This indicates the degenotoxic effect of this bacterial strain pseudomonas resinovorans E20. At the biochemical genetic level, the two treatments (pendimethalin and pseudomonas resinovorans E20) induced some changes in protein banding patterns of Vicia faba plants. These changes included the disappearance of three protein bands with MW′s of 265, 175 and 40 KDa after treatment with the herbicide pendimethalin alone and appearance of the same bands after treatment with Pseudomonas resinovorans E20 bacterial strain.

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