Bacterial Desulfurization of Dibenzothiophene by Pseudomonas Sp. Strain Kwn5 Immobilized in Alginate Beads

Abstract

Biodelfurization of petroleum has emerged as a potential alternative to the hydrodesulfurization and oxidative desulfurization processes. However, the main obstacle in its commercial application is the efficiency and practicality of using bacterial cells. Pseudomonas sp. strain KWN5 was tested for the ability to use dibenzothiophene (DBT) in n-tetradecane as the sole sulfur source with two phase oil-water system. The biodesulfurization ability of strain KWN5 was evaluated by immobilized cells with dibenzothiophene as substrates. The cells immobilized by entrapping them with sodium alginate (SA) had high DBT biodesulfurization activity and could degrade 100 mg DBT/L in n-tetradecane of 46.76–100%, depended on concentrations of sodium alginate and cells within 24 h at 37oC with shaking at 160 rpm. The combination of SA concentration of 3% (w/v) with bacterial cells OD660 40 (25.52 mg DCW/mL) has an optimal biodesulfurization activity on 100 mg DBT/L in n-tetradecane, which is equal to 71.85% biodesulfurization. The immobilized cells of Pseudomonas sp. strain KWN5 in alginate beads were more efficient for the degradation of DBT and can be reused for five cycles (220 h) without any loss in their activity. The results of this study clearly show the role of the effects of cell immobilization in increasing the process of biodesulfurization.

Improvement in desulfurization of dibenzothiophene and dibenzothiophene sulfone by Paenibacillus strains using immobilization or nanoparticle coating

“…Desulfurization of DBT by immobilized bacteria has been measured in other studies using the disappearance of DBT rather than the production of 2-HBP as an end point (Gunam et al, 2021;Guo et al, 2014;Guobin et al, 2005;Peng & Wen, 2010). These studies used initial DBT concentrations similar to those used in the work T A B L E 1 Enhancement (%) of the maximum 2-HBP produced by immobilized and nanoparticle-coated 32O-W and 32O-Y strains at 0.1 and 0.5 mmol l −1 initial DBT and DBTS compared to that produced under the same conditions by planktonic cells (Sar et al, presented here and found, as seen here with both DBT and DBTS, large decreases in residual DBT during culturing.…”

“…Desulfurization of DBT by immobilized bacteria has been measured in other studies using the disappearance of DBT rather than the production of 2‐HBP as an end point (Gunam et al, 2021; Guo et al, 2014; Guobin et al, 2005; Peng & Wen, 2010). These studies used initial DBT concentrations similar to those used in the work presented here and found, as seen here with both DBT and DBTS, large decreases in residual DBT during culturing.…”

Biochemical Analytical Methods of Fossil Fuels Biodesulfurization Treatment

“… GC-FID: GC-FID for quantitative estimation of DBT removal [19] and estimation of sulfur compounds by GC-FID-CP-Sil5CB [20].  GC-FPD: such as GC-14A connected with a column Zebron ZB-1 and an FPD for analysis of the content of DBT and its compounds in the culture [21].…”

Biodesulfurization of Fossil Fuels: Analysis and Prospective

“…Rhodococcus strains, such as R. erythropolis , have been isolated from contaminated soil using basal salts or sulfur-free medium (Jarullah, 2012). Other isolates, such as Gordonia sp., Noocaria sp., Pantoea agglomerans , Pseudomonas delafieldii , Pseudomonas aeruginosa , SRB, and Sphingomonas subarctica , have also been prepared and studied ( Chen et al ., 2019a , 2019b ; Gunam et al ., 2021a , 2021b ; Saeed et al ., 2022 ).…”