Bacterial Detection &amp;amp; Identification Using Electrochemical Sensors

Halford, Colin; Gau, Vincent; Churchill, Bernard M.; Haake, David A.

doi:10.3791/4282

Cited by 6 publications

(3 citation statements)

References 12 publications

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“…Electrochemical detection is a very sensitive method which uses the electrical current that is produced from oxidation or reduction reactions associated with biological processes. These methods may be used for bacterial detection of molecular biomarkers from different pathogenic infections [ 50 , 51 , 52 ].…”

Section: Nanoparticles For Bacterial Detectionmentioning

confidence: 99%

Nanoparticles for Signaling in Biodiagnosis and Treatment of Infectious Diseases

Colino

Millán

Lanao

2018

IJMS

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Advances in nanoparticle-based systems constitute a promising research area with important implications for the treatment of bacterial infections, especially against multidrug resistant strains and bacterial biofilms. Nanosystems may be useful for the diagnosis and treatment of viral and fungal infections. Commercial diagnostic tests based on nanosystems are currently available. Different methodologies based on nanoparticles (NPs) have been developed to detect specific agents or to distinguish between Gram-positive and Gram-negative microorganisms. Also, biosensors based on nanoparticles have been applied in viral detection to improve available analytical techniques. Several point-of-care (POC) assays have been proposed that can offer results faster, easier and at lower cost than conventional techniques and can even be used in remote regions for viral diagnosis. Nanoparticles functionalized with specific molecules may modulate pharmacokinetic targeting recognition and increase anti-infective efficacy. Quorum sensing is a stimuli-response chemical communication process correlated with population density that bacteria use to regulate biofilm formation. Disabling it is an emerging approach for combating its pathogenicity. Natural or synthetic inhibitors may act as antibiofilm agents and be useful for treating multi-drug resistant bacteria. Nanostructured materials that interfere with signal molecules involved in biofilm growth have been developed for the control of infections associated with biofilm-associated infections.

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Section: Nanoparticles For Bacterial Detectionmentioning

confidence: 99%

Nanoparticles for Signaling in Biodiagnosis and Treatment of Infectious Diseases

Colino

Millán

Lanao

2018

IJMS

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“…Electrochemical immunosensors have been reported for the sensitive detection of various biological agents 31, 32. Enzymes are commonly used as labels in electrochemical detection of many bacteria, viruses, toxins and pathogens 33–35. Electrochemical detection of SEB using enzyme labels has been reported by various researchers 36–38.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Immunosensor for Staphylococcal Enterotoxin B (SEB) Based on Platinum Nanoparticles‐Modified Electrode Using Hydrogen Evolution Inhibition Approach

et al. 2014

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In the present study, the electrocatalytic property of platinum for the reduction of protons to hydrogen in acidic medium is explored for the electrochemical detection of Staphylococcal Enterotoxin B (SEB). For this purpose glassy‐carbon electrodes (GCEs) were modified with platinum nanoparticles (Pt NPs) and studies were conducted on effect of copper deposition on the catalytic efficiency of Pt NPs towards reduction of protons. Linear sweep voltammetry (LSV) was performed to determine a negative shift of hydrogen evolution potential at the Pt NPs modified GCEs. In addition to this, indirect sandwiched ELISA procedure was followed on disposable SPEs. Anti‐mouse immunoglobulin G‐alkaline phosphatase (ALP) conjugate was immobilized on the SPE interface leads the hydrolysis of ascorbic acid 2‐phosphatase (AAP) and produce ascorbic acid which is responsible for the deposition of copper on Pt NPs modified GCEs. The potential for hydrogen evolution is negatively shifted with increasing the concentration of SEB over a range from 1.0 ng/mL to 1.0 µg/mL and the detection limit was found to be 1.0 ng/mL. The overall study emphasizes the role of catalytic efficiency of platinum nanoparticles and optimized conditions in providing better analytical performances for biosensors in term of sensitivity, selectivity and reliability.

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“…8. (A) Sensor arrangement of Au/thiol/EDC-NHS and detection mechanism of surface plasmon resonance [67], (B) Sensor arrangement 11-MUA/protein G/antibody and the flow of SPR detection method [58], (C) Au/MUA/EDC-NHS/phage sensor arrangement schematic diagram[40], and (D) Comparison of streptavidin-biotin approach in different scaffold: bare gold, Au/thiol-biotinylated, and Au/disulphide-biotinylated[59]…”

mentioning

confidence: 99%

The Formation and Organization of Self-Assembled Monolayer (SAM) for Escherichia coli Detection: A Review

Muslihati¹,

Basri

Sahdan

et al. 2020

Proceedings of the 2nd International Conference of Science and Technology for the Internet of Things, ICSTI 2019, September 3rd

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SAM is known as a tuneable platform through an ultra-thin, biocompatible, and controlled organic film formed by self-assembled from a strong chemisorption of thiols (R-S-H) or its derivatives (R-OH, R-COO, R-COOH, R-NH2, etc.) onto metal or semiconductor substrate. The monolayer is offering hydrophobic-hydrophilic control depending on their terminal group. These flexibilities were functionalized in order to immobilize the biomolecule like DNA/RNA, antibody/antigen, bacteriophages, and aptamers as a selective receptor to capture E. coli selectively as the most dangerous microorganism in environmental water into an array system called biosensor. A number of methods have been developed to characterize the performance of SAM functionalization in immobilizing biomolecule for E. coli rapid detection and real time in optical and electrochemical (amperometric, potentiometric, and impedimetric). Till date, nano-materials and nanotechnologies has significantly contributed as a key role as in basic platform forming the sensor sensitively. Nano materials offer attractive properties like shape dependent physical and unique size, easy synthesize, easy surface functionalization, and enhancement of an electron transfer. This review will discuss the biomolecule immobilization techniques with SAM and the sensing mechanism.

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Bacterial Detection & Identification Using Electrochemical Sensors

Cited by 6 publications

References 12 publications

Nanoparticles for Signaling in Biodiagnosis and Treatment of Infectious Diseases

Nanoparticles for Signaling in Biodiagnosis and Treatment of Infectious Diseases

Electrochemical Immunosensor for Staphylococcal Enterotoxin B (SEB) Based on Platinum Nanoparticles‐Modified Electrode Using Hydrogen Evolution Inhibition Approach

The Formation and Organization of Self-Assembled Monolayer (SAM) for Escherichia coli Detection: A Review

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