Bacterial disease resistance of transgenic hybrid poplar expressing the synthetic antimicrobial peptide D4E1

Mentag, Rachid; Luckevich, M. D.; Morency, Marie-Josée; Séguin, Armand

doi:10.1093/treephys/23.6.405

Cited by 67 publications

(29 citation statements)

References 36 publications

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“…Transgenic plants from a Populus tremula L. x P. alba L. hybrid that expressed the D4E1 peptide showed a decrease in symptom severity for diseases caused by Agrobacterium tumefaciens and Xanthomonas populi pv. populi (Mentag et al, 2003). The genetic transformation of tobacco with a gene encoding the synthetic antimicrobial peptide D4E1 controlled the growth of Aspergillus flavus and Verticillium dahliae fungi (Cary et al, 2000); whereas the D4E1 gene expression in cotton plants reduced disease symptoms caused by Thielaviopsis basicola (Rajasekaran et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter

Attílio

Filho

Harakava

et al. 2013

Pesq. agropec. bras.

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-The objective of this work was to produce transgenic 'Pêra' and 'Valência' sweet orange plants using the D4E1 gene driven by the Arabidopsis thaliana phloem protein (AtPP2) promoter and to quantify transgene expression in different transformation events. Genetic transformation experiments were carried out with epicotyl segments co-cultivated with Agrobacterium tumefaciens. Six plants from 'Pêra' sweet orange and seven plants from 'Valência' sweet orange were confirmed as different transgenic events by means of the polymerase chain reaction (PCR) and the Southern blot techniques. Transgene expression was quantified using real-time quantitative PCR. D4E1 gene expression levels vary from 5 up to 50 times among different transformation events.Index terms: Citrus sinensis, antimicrobial peptide, tissue-specific promoter. Transformação genética de laranjas doces com o gene D4E1 dirigido pelo promotor AtPP2Resumo -O objetivo deste trabalho foi obter plantas transgênicas de laranja 'Pêra' e 'Valência' por meio do gene D4E1 dirigido pelo promotor Arabidopsis thaliana phloem protein (AtPP2) e quantificar a expressão dos transgenes nos diferentes eventos de transformação. Os experimentos de transformação genética foram realizados com segmentos de epicótilo cocultivados com Agrobacterium tumefaciens. Seis plantas de laranja 'Pêra' e sete plantas de laranja 'Valência' foram confirmadas como distintos eventos transgênicos por meio das técnicas de reação em cadeia da polimerase (PCR) e Southern blot. A expressão dos transgenes foi quantificada por PCR quantitativo em tempo real. Os níveis de expressão do gene D4E1 variam de 5 a 50 vezes entre os diferentes eventos de transformação.Termos para indexação: Citrus sinensis, peptídeo antimicrobiano, promotor tecido-específico.

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Section: Introductionmentioning

confidence: 99%

Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter

Attílio

Filho

Harakava

et al. 2013

Pesq. agropec. bras.

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“…Furthermore, poplar trees transformed with a gene encoding synthetic peptide D4E1 were found to be resistant to bacterial pathogens Agrobacterium tumefaciens and Xanthomonas populi (Ridé) van den Mooter & Swings but not to the fungal pathogen Hypoxylon mammatum (Wahlenb.) P. Karst [46].…”

Section: Discussionmentioning

confidence: 99%

A Gene Encoding Scots Pine Antimicrobial Protein Sp-AMP2 (PR-19) Confers Increased Tolerance against Botrytis cinerea in Transgenic Tobacco

Jaber

Kovalchuk

Raffaello

et al. 2017

Forests

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Both the establishment of sustainable forestry practices and the improvement of commercially grown trees require better understanding of mechanisms used by forest trees to combat microbial pathogens. We investigated the contribution of a gene encoding Scots pine (Pinus sylvestris L.) antimicrobial protein Sp-AMP2 (PR-19) to the host defenses to evaluate the potential of Sp-AMP genes as molecular markers for resistance breeding. We developed transgenic tobacco plants expressing the Sp-AMP2 gene. Transgenic plants showed a reduction in the size of lesions caused by the necrotrophic pathogen Botrytis cinerea. In order to investigate Sp-AMP2 gene expression level, four transgenic lines were tested in comparison to control and non-transgenic plants. No Sp-AMP2 transcripts were observed in any of the control and non-transgenic plants tested. The transcript of Sp-AMP2 was abundantly present in all transgenic lines. Sp-AMP2 was induced highly in response to the B. cinerea infection at 3 d.p.i. This study provides an insight into the role of Sp-AMP2 and its functional and ecological significance in the regulation of plant-pathogen interactions.

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“…Sugarcane transformation by detoxifying agents degraded toxins and provided resistance against various pathogens (Zhang et al, 1999). A synthetic peptide named D4E1 was introduced in cotton and poplar, providing resistance against Thielaviopsis basicola fungus in cotton (Rajasekaran et al, 2007) and some bacteria in poplar (Mentag et al, 2003;Montesinos, 2007). Apple was transformed with a grapevine gene stilbene synthase that codes for a phytoalexin, namely resveratrol, found in grapevine but absent in apples.…”

Section: Transgenic Approachmentioning

confidence: 99%

Modernization in plant breeding approaches for improvingbiotic stress resistance in crop plants

Hussain¹

2015

Turk J Agric For

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Biotic stresses are a potential threat to global food security. The origin of new pathogens and insect races due to climatic and genetic factors is a major challenge for plant breeders in breeding biotic stress resistant crops. Yield losses due to biotic stresses have resulted in 800 million people underfed in the world. Reduced yield due to biotic stresses and increasing food demand put international food security at risk as 70% more food will be required in 2050. This review describes and compares the conventional and molecular genetics methods being used for breeding biotic stress resistant crops. In the past, classical breeding approaches like introduction, hybridization, composite crossing, multiline, and backcross breeding were utilized for this purpose. However, these methods were slow, expensive, and hectic for developing resistance in crops. Furthermore, breakdown of resistance due to fast evolving pathogens could not be coped with using these time consuming methods. Therefore, molecular genetics approaches like mutation, marker assisted selection (MAS), genomics, recombinant DNA technology, targeted induced local lesions in genome (TILLING), and virus induced gene silencing (VIGS) were adapted by breeders to develop effective resistance in crop plants in a shorter time. TILLING, being a nontransgenic method, is expected to become the most powerful tool for this purpose.

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Bacterial disease resistance of transgenic hybrid poplar expressing the synthetic antimicrobial peptide D4E1

Cited by 67 publications

References 36 publications

Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter

Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter

A Gene Encoding Scots Pine Antimicrobial Protein Sp-AMP2 (PR-19) Confers Increased Tolerance against Botrytis cinerea in Transgenic Tobacco

Modernization in plant breeding approaches for improvingbiotic stress resistance in crop plants

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