Bacterial Diversity and Antibiotic Resistance Genes in Wastewater Treatment Plant Influents and Effluents

Voolaid, Veiko; Donner, Erica; Vasileiadis, Sotirios; Berendonk, Thomas U.

doi:10.1002/9781119192428.ch9

Cited by 6 publications

(5 citation statements)

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“…The presence of antibiotic-resistant genes (ARGs), such as tetracycline and beta-lactam resistance genes, have been reported in wastewater (Szczepanowski et al, 2009 ; Karkman et al, 2017 ; Voolaid et al, 2018 ), but the current study represents the first investigation on ARB in greywater. Previous studies on ARB in municipal wastewater (Huang et al, 2012 ; Harnisz et al, 2015 ) reported tetracycline-resistant bacterial levels in the range of 10 2 -10 3 CFU mL −1 , and their findings are consistent with our present results for treated greywater (TGW) in the SYS1 and SYS2 systems at 25°C.…”

Section: Discussionmentioning

confidence: 83%

“…is a particularly suitable genus for monitoring antibiotic resistance in the environment; in fact, until recently, bacterial screening of WWTP influents and effluents usually focused on Acinetobacter spp. (Zhang Y. et al, 2009 ; Voolaid et al, 2018 ). Similarly to our case, in a previous study on treated wastewater, tetracycline-resistant strains of Serratia marcescens and Acinetobacter spp .…”

Section: Discussionmentioning

confidence: 99%

“…However, there is still a lack of evidence about the potential efficacy of actual GW treatment before reuse on ARB abundance and the potential contribution of GW irrigation to the spread of ARB. Understanding the dynamics of ARB and ARGs in the urban water cycle is an increasingly important goal as antibiotic resistance is recognized as one of the most significant human health challenges of the Twenty-first century (WHO, 2012 ; Voolaid et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic-Resistant Bacteria in Greywater and Greywater-Irrigated Soils

et al. 2018

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This study represents the first systematic attempt to evaluate antibiotic-resistant bacteria (ARB) occurrence in treated greywater and the potential spread of these bacteria from the greywater to greywater-irrigated soil. Treated greywater from three recirculating vertical flow constructed wetlands, each located in a household in the central Negev Desert, Israel, was surveyed. The presence of antibiotic-resistant bacteria in raw and treated greywater was investigated with culture and molecular methods, as well as their presence in the corresponding treated-greywater-irrigated soils. Additionally, the effectiveness of chlorination to prevent the spread of ARB was tested. The total count of tetracycline-resistant bacteria significantly increased in the treated greywater, likely due to their concentration on the filter matrix of the treatment systems. Twenty-four strains of tetracycline-resistant bacteria were isolated and identified at the genus level by 16Sr RNA gene sequencing. All the tetracycline-resistant bacteria showed high resistance traits, and some of them presented multiple antibiotic resistances. Six tetracycline resistance genes (coding for efflux and ribosomal resistance mechanisms) and five β-lactamase genes were detected. In 14 of the isolated strains, the gene tet39, which is phylogenetically related to both environmental and clinical strains, was identified. All the tet39 resistant bacteria were positive to at least one of the β-lactamase genes tested. Chlorination was found to be an efficient method to reduce ARB in treated greywater. We concluded that disinfection of treated greywater may reduce the risks not only from the potential presence of pathogens but also from the presence of ARB and antibiotic resistance genes.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibiotic-Resistant Bacteria in Greywater and Greywater-Irrigated Soils

et al. 2018

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“…Treatment of wastewater is one of the most important biotechnological approaches applied to treat domestic and industrial wastewater throughout the world. WWTPs are intended to lower overall bacterial loads in addition to sediments and nutrients 36,37 . Many toxic compounds (chemicals and organics) and bacteria that have been collected in sewage treatment facilities may be discharged into the environment through the effluent from WWTPs.…”

Section: Discussionmentioning

confidence: 99%

“…Representative sequences of every RSV were utilized for taxonomy categorization versus the SILVA SSU Ref NR dataset v.132 32 utilizing trained RDP's naive Bayesian classifier at 99% sequence similarity 33,37 . Instead, ESPRIT-Tree software used taxonindependent clustering 34,34 to group sequences into operational taxonomic units (OTUs) by calculating the k-mer distance across sequences at similar values ranging from 99% (species/strain stage) to 80%.…”

Section: Sequence Processing and Analysismentioning

confidence: 99%

Analysis of Bacterial Community and Its Biodegradation Ability of 17-Β Estradiol in Wastewater Plant in Assiut, Upper Egypt

Ali,

Mohamed Halby,

Salim

et al. 2023

Bulletin of Pharmaceutical Sciences Assiut University

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Wastewater treatment is essential for environmental sanitation in urban environments. Nevertheless, chemical, and organic substances, and microorganisms accumulate in wastewater treatment plants (WWTPs), which may be liberated into the environment via their effluents. This study aimed to analyze the bacterial community at the sewage treatment plant entrance in Assiut, Upper Egypt as well as their degradation ability of 17-β estradiol (E2) as an Isolates were identified by conventional methods and, 16S rRNA gene sequencing. E2 degradation by different strains was assessed using HPLC/UV. Conventional methods and 16S rRNA based amplicon sequencing revealed three bacteria including Bordetella sputigena (ASc1), Pigmentiphaga daeguensis (ASc3), and Stenotrophomonas pavanii (ASc4), and when tested for E2 degradation, they degraded 65.5%, 59%, and 66.4% of E2 (50 mg. L -1 ) respectively. Other bacterial strains were identified using 16S rRNA based amplicon sequencing. Bacteroidetes, Firmicutes and Proteobacteria were the prominent phyla. However, Actinobacteria, Cyanobacteria, Chloroflexi, Caldithrix, and Chlamydiae were detected in lower proportions.

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