Bacterial Diversity and Community Characteristics of the Sinus and Dental Regions in Adults with Odontogenic Sinusitis

Abstract

Background The microbiome plays a crucial role in Odontogenic sinusitis (OS); however, bacterial characteristics of the sinuses and connected dental regions in OS are poorly understood. In this study, nasal secretion were collected from 41 OS patients and 20 simple nasal septum deviation patients, and oral mucosa samples from dental regions were collected from 28 OS patients and 22 impacted teeth extraction patients. DNA was extracted and 16S rRNA sequencing was performed to explore the characteristics and structure of microbiome in sinuses and dental regions of OS patients. Results Alpha diversity of oral and nasal microbiome in OS patients all were higher than that in control. Principal co-ordinates analysis (PCoA) showed that oral samples clustered separately from nasal samples, beta diversity of oral and nasal samples in OS patients were also higher than that in control. Dominant phylum was Bacteroidota in OS patients and Firmicutes in control, either in oral or nasal cavity. Dominant genera in oral microbiome of OS patients were similar to that of control, were Fusobacterium, Porphyromonas and Prevotella, which were close to nasal microbiome structure of OS, while dominant genus in nasal microbiome of control were Staphylococcus, Corynebacterium and Cutibacterium. Co-occurrence network analysis showed decreased microbial connectivity in oral mucosa and nasal secretion samples of OS patients. Conclusions Odontogenic infection promotes structural and functional disorders of nasal microbiome in OS. The interaction of dominant pathogens in nasal and oral regions may promote the development of OS. Our study provides the microbiological aetiology of the nasal and connected dental regions in OS and is expected to provide novel insights into the diagnosis and therapeutic strategies for OS.

Subdural empyema as a complication of odontogenic sinusitis: A case report

“…In addition, local drainage procedures are recommended to minimize infectious conditions. Both early diagnosis and prompt initiation of antibiotic therapy are decisive factors for more favorable clinical outcomes (Giannoni et al, 1997;Szyfter et al, 2018;Yoon & Redmond, 2019;Meshref et al, 2022;Wu et al, 2023).…”