Bacterial diversity assessment of world’s largest sewage-fed fish farms with special reference to water quality: a Ramsar site

Tyagi, Inderjeet; Tyagi, Kaomud; Bhutiani, R.; Chandra, Kailash; Kumar, Vikas

doi:10.1007/s11356-021-13756-2

Cited by 10 publications

(4 citation statements)

References 74 publications

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“…This genus also plays a key role in the metabolic cycle of sulphur and nitrogen (Ross et al 2016). It is positively correlated with dissolved oxygen and negatively correlated with biological and chemical oxygen demands (Tyagi et al 2021). Fish gut microbiota contain relatively few free-living environmental bacteria (Wong and Rawls 2012).…”

Section: Osmotic Stress Alters the Intestinal Microbiota Taxonomic Co...mentioning

confidence: 99%

Salinity significantly affects intestinal microbiota and gene expression in striped catfish juveniles

Hieu

Hằng

Lokesh

et al. 2022

Appl Microbiol Biotechnol

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In the present study, juvenile striped catfish (Pangasianodon hypophthalmus), a freshwater fish species, have been chronically exposed to a salinity gradient from freshwater to 20 psu (practical salinity unit) and were sampled at the beginning (D20) and the end (D34) of exposure. The results revealed that the intestinal microbial profile of striped catfish reared in freshwater conditions were dominated by the phyla Bacteroidetes, Firmicutes, Proteobacteria, and Verrucomicrobia. Alpha diversity measures (observed OTUs (operational taxonomic units), Shannon and Faith's PD (phylogenetic diversity)) showed a decreasing pattern as the salinities increased, except for the phylogenetic diversity at D34, which was showing an opposite trend. Furthermore, the beta diversity between groups was significantly different. Vibrio and Akkermansia genera were affected differentially with increasing salinity, the former being increased while the latter was decreased. The genus Sulfurospirillium was found predominantly in fish submitted to salinity treatments. Regarding the host response, the fish intestine likely contributed to osmoregulation by modifying the expression of osmoregulatory genes such as nka1a, nka1b, slc12a1, slc12a2, cftr, and aqp1, especially in fish exposed to 15 and 20 psu. The expression of heat shock proteins (hsp) hsp60, hsp70, and hsp90 was significantly increased in fish reared in 15 and 20 psu. On the other hand, the expression of pattern recognition receptors (PRRs) were inhibited in fish exposed to 20 psu at D20. In conclusion, the fish intestinal microbiota was significantly disrupted in salinities higher than 10 psu and these effects were proportional to the exposure time. In addition, the modifications of intestinal gene expression related to ion exchange and stressful responses may help the fish to adapt hyperosmotic environment. Key points• It is the first study to provide detailed information on the gut microbiota of fish using the amplicon sequencing method.• Salinity environment significantly modified the intestinal microbiota of striped catfish.• Intestinal responses may help the fish adapt to hyperosmotic environment.

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Section: Osmotic Stress Alters the Intestinal Microbiota Taxonomic Co...mentioning

confidence: 99%

Salinity significantly affects intestinal microbiota and gene expression in striped catfish juveniles

Hieu

Hằng

Lokesh

et al. 2022

Appl Microbiol Biotechnol

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show abstract

“…As shown in Fig.8, C39 was negatively correlated with TN, NH 4 However, it was positively correlated with DO. In contrast, a previous study showed that the genus C39 was negatively correlated with DO(Tyagi et al 2021). BRH-c20a, Run-SP154, Saccharimonadales, Azospira, and Rhodobacter were positively correlated with TN, and Leucobacter was positively correlated with DO.…”

mentioning

confidence: 64%

“…The C39, belonging to Rhodocyclaceae, is the most abundant genus in the aquatic environment (Zhang et al 2019). It is also found in the developmental stages of Chironomus circumdatus, and in the water of the East Kolkata Wetlands (Laviad-Shitrit et al 2021; Tyagi et al 2021). In our study, C39 was the dominant genus in the two ponds in the early stage (Supplementary Figure 2).…”

Section: Bacterial Composition Of the Two Different Ponds At Three Sa...mentioning

confidence: 99%

Comparative analysis of bacterial diversity of pond water with different output of Chinese soft-shelled turtle Pelodiscus sinensis in dark greenhouse mode

Wang,

Chang,

Yan

et al. 2023

Preprint

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Dark greenhouse culture is one of the main culture methods of the Chinese soft-shelled turtle, Pelodiscus sinensis. The present study found that the outputs of P. sinensis differed among five ponds with the same initial stocking density and management measures, with the output of pond B with maximum yield being 1.69 times higher than that of pond A with minimum yield. Therefore, the physicochemical factors and bacterial communities of the two ponds were monitored, analyzed and compared. The contents of total nitrogen, nitrite nitrogen, and dissolved oxygen in pond B were extremely significantly higher than those in pond A at the late stage of cultivation. Correspondingly, the microbial diversity and richness were also higher in pond B. Additionally, the higher relative abundances of Gemmatimonadetes, Firmicutes, Nitrosomonas, Saccharimonadales andAzospira in pond B conferred it higher nitrification and denitrification ability than pond A in the middle and late stage of cultivation. Pearson’s correlation coefficient showed that the Saccharimonadales andAzospira were correlated with total nitrogen, ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen. Our results provide a reference for the modulation of the water environment in the dark greenhouse mode that used for turtle cultivation.

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“…The relative abundance of Cloacibacterium in group L was higher on day 5 than that in group CK and then recovered quickly. Cloacibacterium is closely related to humans and had been found in many environments [ 39 , 40 , 41 ]. One clinical study from 2014 to 2019 on Vagococcus showed that the isolation of Vagococcus was increasing and that Vagococcus isolated from patients was resistant to ampicillin, sulfamethoxazole, vancomycin, and many other kinds of antibiotics [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Doxycycline Attenuates Pig Intestinal Microbial Interactions and Changes Microbial Metabolic Pathways

Liang

Wen

et al. 2023

Animals

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Doxycycline is a therapeutic veterinary antibiotic commonly used in pig breeding. In this study, 27 fattening pigs of 33.5 ± 0.72 kg were divided equally into 3 groups. Doxycycline at 0, 3, and 5 mg/kg body weight was added to the feed in groups CK, L and H. The medication and withdrawal periods were set at 5 and 28 days. The results showed that the doxycycline average concentrations in groups L and H during the medication period were 117.63 ± 13.54 and 202.03 ± 24.91 mg/kg dry matter, respectively. Doxycycline levels were lower than the detection limit after 20 days. Doxycycline did not affect the diversity of the intestinal microbial community structure. The relative abundances of Streptococcus were significantly higher in treatment groups than that in group CK, and Alishewanella, Vagococcus, Cloacibacterium, and Campylobacter abundances were significantly positively correlated with doxycycline concentration. Interestingly, the microbiota cooccurrence network suggested that high doxycycline concentration weakened the interactions among bacteria until day 33. Functional prediction showed that doxycycline significantly altered metabolic pathways related to the cell membrane. The results revealed that the use of doxycycline during pig breeding can affect bacterial abundance during the withdrawal period, and it may affect interactions among bacteria and change the intestinal metabolic pathways.

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Bacterial diversity assessment of world’s largest sewage-fed fish farms with special reference to water quality: a Ramsar site

Cited by 10 publications

References 74 publications

Salinity significantly affects intestinal microbiota and gene expression in striped catfish juveniles

Salinity significantly affects intestinal microbiota and gene expression in striped catfish juveniles

Comparative analysis of bacterial diversity of pond water with different output of Chinese soft-shelled turtle Pelodiscus sinensis in dark greenhouse mode

Doxycycline Attenuates Pig Intestinal Microbial Interactions and Changes Microbial Metabolic Pathways

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