Bacterial diversity dynamics in rumen epithelium of wethers fed forage and mixed concentrate forage diets

Sadet-Bourgeteau, Sophie; Martin, Cécile; Morgavi, Diego P.

doi:10.1016/j.vetmic.2010.04.029

Cited by 40 publications

(47 citation statements)

References 28 publications

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“…2), providing evidence that diet influences the ecology of the epimural bacterial community. Contrary to previous findings (35,36), we observed that transition to the high-grain diets affected the diversity of the epimural bacterial community. The discrepancy between our results and those of past studies may be due to several reasons.…”

Section: Vol 77 2011contrasting

confidence: 99%

“…When our study was compared to a study of sheep by Sadet-Bourgeteau et al (36), the phyla Firmicutes, Bacteroidetes, Proteobacteria, and Spirochaetes were detected from both sheep and heifers, suggesting they are the predominant bacteria in the epimural bacterial community in ruminants. The lower proportion of the phyla Firmicutes and Bacteroidetes (55% in heifers versus 86% in sheep) and the higher proportion of unknown bacteria in our study could be the result of the low resolution of the PCR-DGGE analysis and the numbers of bands identified.…”

Section: Vol 77 2011supporting

confidence: 45%

“…The nature of feed materials and the physicochemical changes induced by their fermentation are known to favor the development of certain microbial ecotypes in the ruminal solid and liquid phases (24). Meanwhile, the diversity of the ruminal epimural microflora has been reported to be stable relative to that in the ruminal contents through 8 weeks (35) and under different dietary regimens (35,36). In this study, we hypothesized that transition from a high-forage diet to a highgrain diet can affect the diversity of epimural bacteria in beef cattle.…”

Section: Discussionmentioning

confidence: 94%

“…The epimural bacteria have been reported to be taxonomically distinct from those in the rumen fluid or bacteria attached to solids in dairy cattle (7, 25) and sheep (5), using culture-based techniques. Recent studies using culture-independent techniques have also confirmed that the epimural bacterial community is distinctly different from the liquid-and particleassociated bacteria (7,27,35,36). However, the ecology of the epimural bacteria and whether diet affects the diversity of this population in the rumens of beef cattle have not been studied.…”

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confidence: 99%

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Changes in Bacterial Diversity Associated with Epithelial Tissue in the Beef Cow Rumen during the Transition to a High-Grain Diet

Chen

Penner

et al. 2011

Appl Environ Microbiol

117

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Our understanding of the ruminal epithelial tissue-associated bacterial (defined as epimural bacteria in this study) community is limited. In this study, we aimed to determine whether diet influences the diversity of the epimural bacterial community in the bovine rumen. Twenty-four beef heifers were randomly assigned to either a rapid grain adaptation (RGA) treatment (n ‫؍‬ 18) in which the heifers were allowed to adapt from a diet containing 97% hay to a diet containing 8% hay over 29 days or to the control group (n ‫؍‬ 6), which was fed 97% hay. Rumen papillae were collected when the heifers were fed 97%, 25%, and 8% hay diets. PCR-denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR analysis were used to characterize rumen epimural bacterial diversity and to estimate the total epimural bacterial population (copy numbers of the 16S rRNA gene). The epimural bacterial diversity from RGA heifers changed (P ‫؍‬ 0.01) in response to the rapid dietary transition, whereas it was not affected in control heifers. A total of 88 PCR-DGGE bands were detected, and 44 were identified from phyla including Firmicutes, Bacteroidetes, and Proteobacteria. The bacteria Treponema sp., Ruminobacter sp., and Lachnospiraceae sp. were detected only when heifers were fed 25% and 8% hay diets, suggesting the presence of these bacteria is the result of adaptation to the high-grain diets. In addition, the total estimated population of rumen epimural bacteria was positively correlated with molar proportions of acetate, isobutyrate, and isovalerate, suggesting that they may play a role in volatile fatty acid metabolism in the rumen.The ruminal bacteria digest complex and simple carbohydrates in the rumen and produce nutrients, such as volatile fatty acids (VFA), microbial protein, and vitamins, for the host. The rumen bacteria have been classified into three groups based upon their locations of colonization within the rumen: those associated with liquid, attached to solid particles, and attached to the ruminal epithelium (defined as epimural bacteria) (4). To date, most studies have focused on the bacteria attached to solid particles and associated with liquid (13,16,23,35,42). These studies have revealed that the bacterial diversity in ruminal contents is highly responsive to changes in diet, age, and antibiotic use, with the health status of the host, geographical location, and season further contributing to variation in diversity (42).While they account for only approximately 1 to 2% of the total bacterial population in the rumen (34), it has been suggested that epimural bacteria play essential roles in oxygen scavenging (6), urea hydrolysis (10, 47), and tissue recycling (25). As epimural bacteria are directly attached to the epithelial lining, they may also be involved in host-microbial interactions, and they may have a role in barrier function for rumen tissue, which is exposed to various fermented products. The epimural bacteria have been reported to be taxonomically distinct from those in the rumen fluid or ...

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Section: Vol 77 2011contrasting

confidence: 99%

Section: Vol 77 2011supporting

confidence: 45%

Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

See 2 more Smart Citations

Changes in Bacterial Diversity Associated with Epithelial Tissue in the Beef Cow Rumen during the Transition to a High-Grain Diet

Chen

Penner

et al. 2011

Appl Environ Microbiol

117

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“…in the rumen wall may be involved in CH 4 oxidation. However, their presence in the rumen epithelium was not always observed suggesting that they are minor or occasional members (Sadet-Bourgeteau et al, 2010;Li et al, 2011).…”

Section: Methylotrophsmentioning

confidence: 93%

The use of direct-fed microbials for mitigation of ruminant methane emissions: a review

Jeyanathan

Martin

Morgavi

2014

Animal

Self Cite

132

115

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Concerns about the environmental effect and the economic burden of methane (CH 4 ) emissions from ruminants are driving the search for ways to mitigate rumen methanogenesis. The use of direct-fed microbials (DFM) is one possible option to decrease CH 4 emission from ruminants. Direct-fed microbials are already used in ruminants mainly to increase productivity and to improve health, and are readily accepted by producers and consumers alike. However, studies on the use of DFM as rumen CH 4 mitigants are scarce. A few studies using Saccharomyces cerevisiae have shown a CH 4 -decreasing effect but, to date, there has not been a systematic exploration of DFM as modulators of rumen methanogenesis. In this review, we explored biochemical pathways competing with methanogenesis that, potentially, could be modulated by the use of DFM. Pathways involving the redirection of H 2 away from methanogenesis and pathways producing less H 2 during feed fermentation are the preferred options. Propionate formation is an example of the latter option that in addition to decrease CH 4 formation increases the retention of energy from the diet. Homoacetogenesis is a pathway using H 2 to produce acetate, however up to now no acetogen has been shown to efficiently compete with methanogens in the rumen. Nitrate and sulphate reduction are pathways competing with methanogenesis, but the availability of these substances in the rumen is limited. Although there were studies using nitrate and sulphate as chemical additives, use of DFM for improving these processes and decrease the accumulation of toxic metabolites needs to be explored more. There are some other pathways such as methanotrophy and capnophily or modes of action such as inhibition of methanogens that theoretically could be provided by DFM and affect methanogenesis. We conclude that DFM is a promising alternative for rumen methane mitigation that should be further explored for their practical usage.

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Rumen Bacteria

Zhou

Chen

Guan

2015

Rumen Microbiology: From Evolution to Revolution

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Bacterial diversity dynamics in rumen epithelium of wethers fed forage and mixed concentrate forage diets

Cited by 40 publications

References 28 publications

Changes in Bacterial Diversity Associated with Epithelial Tissue in the Beef Cow Rumen during the Transition to a High-Grain Diet

Changes in Bacterial Diversity Associated with Epithelial Tissue in the Beef Cow Rumen during the Transition to a High-Grain Diet

The use of direct-fed microbials for mitigation of ruminant methane emissions: a review

Rumen Bacteria

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