Bacterial Diversity in an Amazonian Mangrove Ecosystem

Abstract

Mangroves form a transition zone among terrestrial, marine, and freshwater environments, and they host a striking diversity of macro-and microorganisms. In recent years, increasing anthropogenic impacts have caused widespread degradation of mangrove environments. To analyze the bacterial diversity of Salina Lake, an artificial body of water located in the mangroves of eastern Amazonia, total DNA was extracted from sediment and water samples that were collected from three sites (P1, P2, P3) located within the lake. The 16S rRNA gene was partially amplified, separated using the Denaturing High Performance Liquid Chromatography (DHPLC) method, and sequenced. The DHPLC profiles were quite distinct for the different aquatic and sedimentary bacterial communities. The most abundant groups (> 70%) at all sampled locations were Proteobacteria and unclassified bacteria, but other phyla were also identified, including Bacteroidetes, Cyanobacteria, Acidobacteria, Firmicutes, Actinobacteria, Chloroflexi, Siprochaetes and TM7. A total of 24, 15, and 23 OTUs (Operational Taxonomic Units) were observed in the water samples from P1, P2, and P3, respectively, whereas the sediment samples returned 29, 34, and 52 OTUs. This is the first analysis performed on the bacterial diversity of Salina Lake, which was created by the construction of a highway within a protected mangrove area.

Metagenomic Insights on Soil Microbiome Biodiversity From an Eroding Coastline of Tanjung Piai, Johor State Park, Malaysia

“…The distribution of Proteobacteria as the most dominant phyla, followed by Chloroflexi, Bacteroidetes and Bathyarchaeota in mangrove sediments (Figures 1 and 2) were concurrent with other findings in Malaysia (Ismail et al 2017, Jeyanny et al 2020 and in other subtropical and tropical countries (Dos Santos et al 2011, Pureza et al 2012. Proteobacteria The inter-relationship needs to be further explored with regards to its prominence in the PM2 and PM3 samples (Figures 1, 2 and 5) as some archaea are known to adapt to acidic conditions and others to neutral to slightly alkaline conditions (Bengtson et al 2012).…”

Semiconductor Sequencing Reveals the Diversity of Bacterial Communities in an Amazonian Reservoir

“…However, few studies have been performed in the Amazon region (da C Jesus, Marsh, Tiedje, & de S Moreira, 2009; Rodrigues et al, 2013), as most of these studies still use the Sanger method (Baraúna et al, 2012;Borneman & Triplett, 1997;Graças et al, 2011Graças et al, , 2013Navarrete, Cannavan, Taketani, & Tsai, 2010;Pazinato, Paulo, Mendes, Vazoller, & Tsai, 2010;Pureza et al, 2013). If the continental dimensions, large heterogeneity and high complexity of the Amazon region are taken into consideration, we assume that little is known about the diversity of its environments.…”