Bacterial diversity in an industrial wastewater bioreactor

Bramucci, M G; Kane, Helene; Chen, M.; Nagarajan, Vasantha

doi:10.1007/s00253-003-1372-x

Cited by 31 publications

(29 citation statements)

References 16 publications

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“…It is suggested that by substituting activated sewage for the sewage-derived inoculum, little potential for biodegradation will be lost. In addition, we have shown that the sewage-derived bacterial consortium is composed of bacteria typically present in activated sewage (Bramucci and Nagarajan, 2000;Bramucci et al, 2003;Kavanaugh and Randall, 1994). Whilst activated sewage will contain organisms in addition to those isolated in this study, we have shown that the cultured consortium contains a range of bacteria representative of those found in the parent inoculum.…”

Section: Discussionmentioning

confidence: 86%

Evaluation and Modification of the OECD 301F Respirometry Biodegradation Test Method with Regard to Test Substance Concentration and Inoculum

O’Malley¹

2006

Water Air Soil Pollut

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The majority of OECD (Organisation for Economic Co-operation and Development) ready biodegradation test methods require a relatively high concentration of test substance. Such high dosage concentrations are unsuitable for screening of inhibitory or biocidal compounds. Unfortunately, the method with the lowest dosage concentration (Closed Bottle Test, 301D) also has the lowest biodegradation potential. Therefore, we have evaluated the ability of the Manometric Respirometry (301F) test method to study biodegradation of test substances at concentrations of a few mgl −1 , using sodium acetate as a model substance. Results have been compared to those obtained from closed bottle tests (301D). Problems with using activated sewage as an inoculum have been demonstrated, as respirometry curves were biphasic, and the recorded biological oxygen demand (BOD) was higher than the calculated theoretical oxygen demand (ThOD). A consortium of bacteria isolated from activated sewage was used as an alternative inoculum. Using the alternative inoculum for respirometry tests, monophasic growth curves were observed with recorded BOD values at expected levels. Sensitivity thresholds for the respirometer tests were comparable to those in closed bottles, with biodegradation being detected at concentrations as low as 2.5 mgl −1 ThOD. Comparison of whole community carbon source utilisation profiles for both inocula (obtained using BIOLOG microplates) revealed their catabolic potential to be similar. Therefore, use of an activated sewagederived inoculum is recommended as an alternative inoculum for respirometer-based biodegradation testing.

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Section: Discussionmentioning

confidence: 86%

Evaluation and Modification of the OECD 301F Respirometry Biodegradation Test Method with Regard to Test Substance Concentration and Inoculum

O’Malley¹

2006

Water Air Soil Pollut

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“…The results of the present study show that the 16S rDNA clone libraries have high coverage, and can adequately represent the diversity of bacteria present in communities; 92% and 97% coverage was achieved for the two tanks, respectively (Table 4), and in other studies coverage has ranged from 28% to 93% (29). Bacterial diversity in wastewater treatment bioreactors has been examined in many studies (5,7,8,18,24). Findings from these studies suggest that the most frequently found types of bacteria are the β-proteobacteria.…”

Section: Resultsmentioning

confidence: 99%

“…Bramucci et al (5) surveyed bacterial diversity in an industrial wastewater bioreactor using 16S rDNA analysis and obtained 106 OTUs from 407 rDNA sequences. In a review of current research on microbial community composition and function in wastewater treatment plants, Wagner et al (29) noted that systems with low levels of functional redundancy (due to low levels of diversity for bacteria in certain functional groups) are more sensitive to failure than systems with high levels of functional redundancy (due to high levels of diversity in the same bacterial group).…”

Section: Resultsmentioning

confidence: 99%

“…The resulting RFLP products were separated by gel electrophoresis in 3% agarose. Colonies that produced the same RFLP pattern (DNA fragments of the same size) were grouped together and considered members of the same operational taxonomic units (OTUs) (5), and the frequency of each OTU was used as an indicator of abundance. Each 16S rDNA sequence was assigned to an OTU for microbial community analysis to gain insight into the biodiversity of the bioreactor.…”

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confidence: 99%

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Analysis of Bacterial Community Structure in the Natural Circulation System Wastewater Bioreactor by Using a 16S rRNA Gene Clone Library

Niu

Fukushima

Jiang

et al. 2006

Microbiology and Immunology

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A variety of physical and chemical parameters are routinely monitored during operation of the Natural Circulation System, a wastewater purification bioreactor in which only natural materials and no synthetic chemicals are used. However, the microbial community structures existing in the Natural Circulation System have not been well characterized. Thus, bacterial community structure and composition in this system were studied using clone library analysis of 16S ribosomal RNA genes amplified using PCR with universal bacterial primer sets. The PCR products were then subcloned into the pGEM‐T vector. Each unique restriction fragment length polymorphism pattern, created by using two pairs of restriction endonucleases, was designated as an operational taxonomic unit (OTU). The Natural Circulation System comprises five tanks, the second and third of which play a major role in the bioreactor. Clone library profiles and principal component analysis revealed differences in the bacterial community structures of the second (anaerobic chamber) and the third (aerobic chamber) tanks. However, the β‐proteobacteria, Bacteroidetes/Chlorobi and γ‐proteobacteria groups were dominant in both tanks. Bacterial composition was more complex in the second tank (107 OTUs) than in the third tank (68 OTUs). Of a total of 154 OTUs in the clone libraries, only 21 were common to the two tanks. The results obtained in this study should provide important information for future research into and management of the Natural Circulation System wastewater bioreactor.

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“…Alphaproteobacteria was the dominant bacterium in activated sludge exposed to 620.5 mg/L of OTC, followed by Betaproteobacteria, Actinobacteria, and Firmicutes. Previous research reported that Betaproteobacteria was an important bacterial group in aerobic reactors (Bramucci et al 2003) and dominated OTC production wastewater treatment systems (Liu et al 2012), while Alphaproteobacteria was shown to dominate in 65 % of 86 investigated industrial wastewater treatment plants (Kragelund et al 2006). The increased presence of Actinobacteria and Firmicutes may relate to their ability to easily acquire antibiotic resistance.…”

Section: Discussionmentioning

confidence: 96%

Response of activated sludge to the treatment of oxytetracycline production waste stream

Liu

Zhang

Ding

et al. 2012

Appl Microbiol Biotechnol

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To investigate how the microbial community in activated sludge responded to high antibiotic levels, a benchscale aerobic wastewater treatment system was used to treat oxytetracycline (OTC) mother liquor (OTC-ML). Removal efficiency of chemical oxygen demand decreased from 64.9 to 51.0 % when the OTC level increased from 191.6 to 620.5 mg/L, respectively. According to the cloning results, Psychrobacter and Cryptophyta were the dominant bacterium and eukaryote in the inoculated sludge, respectively, both of which related to low temperature. After OTC exposure, Alphaproteobacteria and Betaproteobacteria became the dominant bacteria, with a small proportion of Firmicutes, Actinobacteria appeared, and fungi (mainly Saccharomycotina) became the dominant eukaryotes, indicating the possible functions of these microorganisms in the wastewater treatment of OTC-ML. The relative abundance of nine tetracycline resistance genes and four mobile elements (class 1 integron, class 2 integron, transposon Tn916/1545, and pattern 1 insertion sequence common region) significantly increased from undetectable to 2.1×10 −3 in the inoculated sludge to 1.7×10 −4 -9.8×10 −1 in sludge exposed to 620.5 mg/L OTC by using real-time PCR. The variety of gene cassette arrays of class 1 integron in the sludge samples increased with increasing OTC exposure concentration.

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Bacterial diversity in an industrial wastewater bioreactor

Cited by 31 publications

References 16 publications

Evaluation and Modification of the OECD 301F Respirometry Biodegradation Test Method with Regard to Test Substance Concentration and Inoculum

Evaluation and Modification of the OECD 301F Respirometry Biodegradation Test Method with Regard to Test Substance Concentration and Inoculum

Analysis of Bacterial Community Structure in the Natural Circulation System Wastewater Bioreactor by Using a 16S rRNA Gene Clone Library

Response of activated sludge to the treatment of oxytetracycline production waste stream

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