Bacterial diversity of polluted surface sediments in the northern Adriatic Sea

Korlević, Marino; Žučko, Jurica; Dragic, Mirjana Najdek; Blažina, Maria; Pustijanac, Emina; Zeljko, Tanja Vojvoda; Gaćeša, Ranko; Baranašić, Damir; Starčević, Antonio; Diminić, Janko; Long, Paul F.; Cullum, John; Hranueli, Daslav; Orlić, Sandi

doi:10.1016/j.syapm.2015.03.001

Cited by 42 publications

(35 citation statements)

References 50 publications

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“…The viscosity of petroleum decreased by 70.8% after being degraded by Q8 for 7 days, and the degradation rate reached 138 mg/day. The petroleum emulsified and dispersed in the biodegradation system indicated that crude oil could dissolve in water and was easy to utilize [38,39,40]. …”

Section: Resultsmentioning

confidence: 99%

Removal Capacities of Polycyclic Aromatic Hydrocarbons (PAHs) by a Newly Isolated Strain from Oilfield Produced Water

Wang

et al. 2017

IJERPH

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The polycyclic aromatic hydrocarbon (PAH)-degrading strain Q8 was isolated from oilfield produced water. According to the analysis of a biochemical test, 16S rRNA gene, house-keeping genes and DNA–DNA hybridization, strain Q8 was assigned to a novel species of the genus Gordonia. The strain could not only grow in mineral salt medium (MM) and utilize naphthalene and pyrene as its sole carbon source, but also degraded mixed naphthalene, phenanthrene, anthracene and pyrene. The degradation ratio of these four PAHs reached 100%, 95.4%, 73.8% and 53.4% respectively after being degraded by Q8 for seven days. A comparative experiment found that the PAHs degradation efficiency of Q8 is higher than that of Gordonia alkaliphila and Gordonia paraffinivorans, which have the capacities to remove PAHs. Fourier transform infrared spectra, saturate, aromatic, resin and asphaltene (SARA) and gas chromatography–mass spectrometry (GC–MS) analysis of crude oil degraded by Q8 were also studied. The results showed that Q8 could utilize n-alkanes and PAHs in crude oil. The relative proportions of the naphthalene series, phenanthrene series, thiophene series, fluorene series, chrysene series, C21-triaromatic steroid, pyrene, and benz(a)pyrene were reduced after being degraded by Q8. Gordonia sp. nov. Q8 had the capacity to remediate water and soil environments contaminated by PAHs or crude oil, and provided a feasible way for the bioremediation of PAHs and oil pollution.

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Section: Resultsmentioning

confidence: 99%

Removal Capacities of Polycyclic Aromatic Hydrocarbons (PAHs) by a Newly Isolated Strain from Oilfield Produced Water

Wang

et al. 2017

IJERPH

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“…This had low levels of aliphatic hydrocarbons (resolved n-alkanes 4.13 μg/g dry mass (dm), unresolved complex mixture 22.48 μg/g dm) and PAHs (0.08 μg/g dm) compared to the two polluted sites BN and TV (resolved n-alkanes 38.07 and 6.59 μg/g dm, unresolved complex mixture 518. 19 and 10.75 μg/g dm, PAHs 73.53 and 0.40 μg/g dm) previously reported (1). This sample was used to construct the metagenomic library MET2.…”

Section: Resultsmentioning

confidence: 99%

“…The metagenome MET1 was derived from a heavily polluted sediment sampled from the Uljanik ship repair yard (marked as BN on Fig. 1, BN 44.866665°N 13.840400°E) as previously described (1). The second metagenome MET2 was derived from an unpolluted coastal surface sediment sample from Cuvi beach at Rovinj (marked as CU on Fig.…”

Section: Methodsmentioning

confidence: 99%

“…1, 45.276365°N 14.549654°E) (1). These two samples had been enriched for potential hydrocarbon-degrading bacteria by incubating under aerobic or anaerobic conditions in the presence of crude oil (1). The sampling procedure for sample CU and the assay for hydrocarbon content were as previously described for the samples BN and TV (1).…”

Section: Methodsmentioning

confidence: 99%

“…In order to establish a baseline for future studies, the concentrations of hydrocarbons in surface sediments and the bacterial diversity at seven selected sites were studied (1). This paper expands the previous study relying on bioprospecting, process of systemic searching for genetic and biochemical potential of bacterial communities, for hydrocarbon degradation activity.…”

Section: Introductionmentioning

confidence: 91%

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Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments

Baranašić

Starčević

Diminić

et al. 2018

Food Technol. Biotechnol.

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SummaryThree metagenomic libraries were constructed using surface sediment samples from the northern Adriatic Sea. Two of the samples were taken from a highly polluted and an unpolluted site respectively. The third sample from a polluted site had been enriched using crude oil. The results of the metagenome analyses were incorporated in the REDPET relational database (http://redpet.bioinfo.pbf.hr/REDPET), which was generated using the previously developed www.ftb.com.hrPlease note that this is an unedited version of the manuscript that has been accepted for publication. This version will undergo copyediting and typesetting before its final form for publication. We are providing this version as a service to our readers. The published version will differ from this one as a result of linguistic and technical corrections and layout editing.2 MEGGASENSE platform. The database includes taxonomic data to allow the assessment of the biodiversity of metagenomic libraries and a general functional analysis of genes using hidden Markov model (HMM) profiles based on the KEGG database. A set of 22 specialised HMM-profiles was developed to detect putative genes for hydrocarbon-degrading enzymes.Use of these profiles showed that the metagenomic library generated after selection on crude oil had enriched genes for aerobic n-alkane degradation. The use of this system for bioprospecting was exemplified using potential alkB and almA genes from this library.

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Microbial Ecology of Marine Environments Chronically Polluted by Petroleum

Cravo‐Laureau¹,

Duran²

2019

Microbial Communities Utilizing Hydrocarbons and Lipids: Members, Metagenomics and Ecophysiology

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Bacterial diversity of polluted surface sediments in the northern Adriatic Sea

Cited by 42 publications

References 50 publications

Removal Capacities of Polycyclic Aromatic Hydrocarbons (PAHs) by a Newly Isolated Strain from Oilfield Produced Water

Removal Capacities of Polycyclic Aromatic Hydrocarbons (PAHs) by a Newly Isolated Strain from Oilfield Produced Water

Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments

Microbial Ecology of Marine Environments Chronically Polluted by Petroleum

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