Bacterial diversity of tilapia (Oreochromis niloticus) cultured in brackish water in Saudi Arabia

Al-Harbi, Ahmed H.; Uddin, Naim

doi:10.1016/j.aquaculture.2005.01.026

Cited by 129 publications

(90 citation statements)

References 15 publications

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“…The recovery of Listeria spp. involved pre-enrichment on Listeria primary selective enrichment broth base (Oxoid, CM0863) supplemented with Listeria primary selective enrichment supplement (Oxoid, SR0142E) (UVM I) following incubation at 30 °C for 24 h and Listeria secondary selective enrichment broth base CM0863 supplemented with Listeria secondary selective enrichment supplement (Oxoid, SR0143E) (UVM II) followed by cultures on PALCAM agar following incubation at 30 °C for 48 h as recommended by [10].The number of colonies was recorded before a random assortment was picked, and purified by streaking and restreaking on tryptone soya agar (TSA; Oxoid) plates supplemented with 1% (w/v) sodium chloride [= TNA] with incubation at 30 °C for 48 h [11].Carnobacterium maltaromaticum isolates [from smoked salmon] were grown routinely on de Man Rogosa and Sharpe agar (MRS; Oxoid) at 30 °C for 48 h. Stock cultures were stored in tryptone soya broth (TSB; Oxoid) supplemented with 1% (w/v) sodium chloride [= TNB] and 20% (v/v) glycerol at -70 °C [12].…”

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confidence: 99%

Bacterial Isolates from Seafood in Scotland

NgoziIzuchukwu¹

2017

IOSRJPBS

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I IntroductionFishery products are significant for human nutrition worldwide [1], but may also serve as a source of foodborne pathogens [2]. Chemicals, metals, marine toxins, and infectious agents, .i.e. bacteria, viruses, and parasites, have been detected in seafood, and may be associated with mild gastroenteritis to life-threatening diseases [3]. Microbial contamination of fish tissues may reflect the presence of pollutants in the aquatic environment [4]. This contamination by pathogens poses a potential risk to human health [5]. Specifically, the consumption of raw or undercooked seafood is especially problematic to human health. Filter feeders, such as mussels, and oysters, are particularly troublesome in terms of microbial contamination insofar as the animals collect bacteria in their filtering systems. Thus, they entrap pathogenic and non-pathogenic bacteria and viruses that occur in the aquatic environment [6].The presence of putative pathogens, such as Vibrio parahaemolyticus, Listeria monocytogenes, Staphylococcus aureus, Clostridium perfringens and Edwardsiella tarda,have been documented in conger eel, swordfish, sole, grouper, and whiting [2]. This study attempts has focused on potential pathogenic and spoilage microorganisms isolated from seafood in Central Scotland. II Materials And Methods Sample collectionThe seafood samples were obtained from Edinburgh and Stirling, Scotland, UK from March to April, 2010. Thus, fresh salmon and haddock fillets, chosen from within the middle of stacks of fillets in chilled display cabinets within fishmongers, were collected by use of disposable plastic gloves and packed in sealed aluminium foil packs. Enquiries of the fishmongers confirmed that in all cases the fish had been landed from fishing boats within the previous 24 h, although it was unknown how long the fish had been on the boats after capture. Filleting of the whole fish occurred in the shops within the same day as purchase, and the fillets were immediately displayed in refrigerated cabinets. Smoked haddock fillets were obtained by the fishmongers from a commercial smoke house in Leith, Edinburgh. The smoked salmon was pre-packed, and was sourced from farmed Atlantic salmon that had been smoked in the Highlands of Scotland. There was a lack of clarity about the origin of bags of live mussels and oysters, which were obtained by the fishmongers from wholesalers. All animals were sourced from Scotland. There was not any stated shelf life for any of the fresh fish or shellfish purchases.Samples from fishmongers in Stirling were packed in polyethylene wraps, and stated to be fresh with a 24 h use-by date. The samples from Edinburgh were transported to the laboratory within one hour of collection, and were maintained in a cold box at 4 o C. The samples from Stirling were transported at room temperature, and were processed microbiologically within 20 min of collection. In total, 4 samples of cold smoked salmon, 4 samples of fresh salmon, 4 bags of mussels, one sample of cold smoked haddock, one sample of fresh ...

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confidence: 99%

Bacterial Isolates from Seafood in Scotland

NgoziIzuchukwu¹

2017

IOSRJPBS

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“…Although gut microbiota has become an integral component of the host, and received increasing attention [18], the fungal diversity in fi sh gut fl ora is not still reported [10]. The fi sh gut microbiota is only focused on bacteria [7][8][9][10][11][12][13][14][15][16][17][18][19]. Our results showed that the hindguts contained more diverse fungi than those in foreguts.…”

Section: Discussionmentioning

confidence: 64%

“…The Shewanella also been found in intestinal contents of goldfi sh, yellow catfi sh, rainbow rout [8,11,17]. The sulphate-reducing bacteria Desulfovibrio had not been detected in fi sh guts [7][8][9][10][11][12][13][14][15][16][17][18][19], although the Desulfovibrio are the most routinely recovered from animal and human faeces [37]. The results illustrated that the intestinal fl ora varied after the live crucian carp was caught and maintained in the tanks.…”

Section: Discussionmentioning

confidence: 98%

“…Most previous studies used traditional culture-dependent or DGGE, clone library methods to investigate the fi sh intestinal Citation: Wang bacterial diversity [11][12][13][14][15][16][17]. With the development of the next-generation sequencing technologies, high-throughput sequencing platform is available for characterizing the bacterial community in the gut of fi sh [10,[18][19][20].…”

Section: Introductionmentioning

confidence: 99%

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Illumina Based Analysis of Bacterial and Fungal Flora in Foreguts and Hindguts of Crucian Carp (Carassius Aumtus) in Retail Markets

Wang¹,

Yin²,

Tan³

et al. 2017

Open J Bac

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The intestinal microbiota of fi sh are the major cause for spoilage. To elucidate the bacterial and fungal community of guts, the bacterial and fungal taxa in foreguts and hindguts of crucian carp (Carassius aumtus) were analyzed by Illumina-based sequencing. Results suggested that the foreguts contained more diverse bacteria than those in hindguts; nevertheless, the hindguts contained more diverse fungi than foreguts. The anaerobic bacterial genera Cetobacteri Desulfovibrio and Shewanella in foreguts were still detected in hindguts. The fungal taxa in foreguts were different from those in hindguts. The dominant fungal genera Alternaia (78.6%), Massarina (0.8%) and Fusarium (0.2%) were only detected in hindguts. It was considered that the Alternaria, Emericella, and Cochliobolus might derive from the diets of crucian carp. The activities of Desulfovibrio might be responsible for the changes in odour, fl avor and texture of the fi sh meat. The H 2 S produced by Desulfovibrio is potentially a major toxin to the fi sh gut epithelium and promoted the fi sh spoilage processes. The results are helpful for manipulation of intestinal fl ora to preserve fresh crucian carp in tanks.

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“…Table 1. Total viable bacteria (CFU/ml) in the water of freshwater fish aquaponic system during the study period Aquatic microorganisms not only affect the water quality but are also associated with the fish physiological status, diseases and postharvest quality (Al-Harbi & Uddin, 2005). According to the biochemical tests and API 20 E strip kits used in the identification, there was a prevalence of Aeromonas hydrophila, Pseudomonas fluorescens, Plesiomonas shigelloides, Escherichia coli, Acinetobacter baumannii, Salmonella sp., Staphylococcus sp., Micrococcus sp.…”

Section: Resultsmentioning

confidence: 99%

Investigation of Bacteria and Fish Pathogenic Bacteria Found in Freshwater Aquaponic System

Chitmanat¹,

Pimpimol²,

Chaibu³

2015

JAS

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Bacteria are very vital organisms in aquaponic system decomposing and converting the toxic components of the fish waste into usable vegetable nutrients; however, some unwanted bacteria could cause fish diseases. The purposes of this research were to compare the amount of bacteria in water from aquaponic catfish cultured in different stocking densities and Denaturing Gradient Gel Electrophoresis (DGGE) of Polymerase Chain Reaction (PCR)-amplified 16S rDNA was applied to characterise the bacterial populations. Three different stocking densities; 80, 100 and 120 fish/m 2 of hybrid catfish were cultured in aquaponic system. Each treatment contained 3 replications; Treatment 1, 360 catfish (80 fish/m 2 , without lettuce); Treatment 2, 360 catfish (80 fish/m 2 , without lettuce); Treatment 3, 450 catfish (100 fish/m 2 ); and treatment 4, 540 catfish (120 fish/m 2 ). This research was conducted for 4 months. Water was sampled two times before fish stocking, it was found that the number of bacteria in the first week were higher than the third week. The average number of bacteria in water from treatment 1, 2, 3 and 4 were (2.49 ± 0.40) ×10 7 , (2.43 ± 0.23) ×10 Colonies forming unit (CFU)/ml respectively. According to four-time samplings after catfish were stocked, the average number of bacteria in water from treatment 1, 2, 3 and 4 were (1.85 ± 0.53) ×10 7 CFU/ml respectively. There were no differences in bacterial counts among treatments (p > 0.05). In addition, no significant difference was observed in the number of bacteria in filter tanks (p > 0.05) and the number were quite similar to the ones in catfish tanks. Aeromonas hydrophila and Flavobacterium columnare infected catfish at the beginning of the experiment; however, it got better and no more death found after acclimation. The DGGE analysis of PCR-amplified 16S rDNA fragments revealed that bacterial populations in water were dominated by Aeromonas spp., Pseudomonas spp. and Staphylococcus spp. Antibiotic sensitivity test indicated that enrofloxacin might be the best option for bacterial infection treatment. The results indicate that aquaponic system could reduce the wastewater from catfish culture; however, maintaining a good water quality environment for fish culture should take into account.

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Bacterial diversity of tilapia (Oreochromis niloticus) cultured in brackish water in Saudi Arabia

Cited by 129 publications

References 15 publications

Bacterial Isolates from Seafood in Scotland

Bacterial Isolates from Seafood in Scotland

Illumina Based Analysis of Bacterial and Fungal Flora in Foreguts and Hindguts of Crucian Carp (Carassius Aumtus) in Retail Markets

Investigation of Bacteria and Fish Pathogenic Bacteria Found in Freshwater Aquaponic System

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