Bacterial diversity, physicochemical and geothermometry of South Asian hot springs

Najar, Ishfaq Nabi; Sharma, Prayatna; Das, Sayak; Sherpa, Mingma Thundu; Kumar, Santosh; Thakur, Nagendra

doi:10.1016/j.crmicr.2022.100125

Cited by 8 publications

(4 citation statements)

References 52 publications

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“…J. Microbiol. 58 (2023) OHUD D. AL-HARTHY et al Natural habitats like hot springs and geysers, where hot groundwater emerges due to the Earth's mantle heat, exhibit distinctive and extreme physicochemical properties influenced by various factors (Bisht et al, 2011;Narsing Rao et al, 2021;Najar, 2022). The potential of hot springs as a reservoir for thermophilic bacteria has been investigated in numerous countries, including Saudi Arabia, China, India, Japan, the Philippines, Indonesia, Iceland, and Kamchatka (Mehta & Satyanarayana, 2013;Selim et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Within this category, thermophiles and hyperthermophiles excel in high-temperature environments (Berenguer, 2011). Natural habitats like hot springs and geysers, where hot groundwater emerges due to the Earth's mantle heat, boast distinctive and extreme physicochemical properties influenced by various factors (Bisht et al, 2011;Narsing Rao et al, 2021;Najar, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Thermostable Amylase from Cytobacillus firmus: Characterization, Optimization, and Implications in Starch Hydrolysis

Al-Harthy,

Hozzein,

Abdelkader

et al. 2024

Egypt. J. Microbiol.

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H OT springs are known to be rich in diverse microorganisms, including thermophilic bacteria capable of producing industrially important enzymes. This study aimed to isolate, purify, and identify thermophilic bacteria from three hot springs located in the southern region of Saudi Arabia, namely Al-Ma'a Al-Har, Al-Aredhah, and Al-Ahsarai hot spring. The isolated bacterial isolates were screened for amylase enzyme production, and the most potent isolate, Cytobacillus firmus OQ834432 isolate OHA8, was selected for large-scale production of the amylase enzyme. The enzyme was then purified using salt precipitation, Sephacryl S-200 chromatography, and DEAE-Sepharose column chromatography. A total of twenty-one bacterial species were identified, with Cytobacillus firmus exhibiting the highest amylase activity. The optimal conditions for the amylase enzyme activity were found to be at 50°C and pH 5.0, with a 153% enhancement in activity in the presence of magnesium ions. The enzyme's activity was inhibited by EDTA and was found to be active with starch and amylopectin at 100% and 110.3%, respectively. In conclusion, this study demonstrates the potential of thermophilic bacteria from hot springs to produce industrially important enzymes and provides insights into the optimal conditions for enzyme production and activity. These findings have significant implications for the development of biotechnological applications utilizing thermophilic bacteria in various industrial sectors.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Thermostable Amylase from Cytobacillus firmus: Characterization, Optimization, and Implications in Starch Hydrolysis

Al-Harthy,

Hozzein,

Abdelkader

et al. 2024

Egypt. J. Microbiol.

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“…As geothermal springs are unique sites for extremophilic microorganisms, studies of the microorganisms in these systems have been essential to our understanding of the diversity and evolution of life on Earth [ 2 , 8 , 11 ]. In the past decade, the diversity of microorganisms in hot spring ecosystems has been studied, mainly with a focus on the prokaryotes and fungi [ 12 , 13 ]; such studies have been conducted on hot springs in Yellowstone National Park [ 14 ], the Taupō Volcanic Zone in New Zealand [ 8 ], Tengchong in China [ 3 , 15 ], the Himalayan region in Pakistan [ 2 ], the Great Basin in the United States [ 16 ], Kamchatka in Russia [ 17 ], Malaysia [ 18 ] and the Himalayan region in China [ 4 , 5 , 19 , 20 ]. These studies have provided important insights into the diversity and adaptation mechanisms of microorganisms in these extreme environments.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the protist diversity and community composition of hot springs are far from clear. The HGB is one of the most hot-spring-rich regions in the world [ 13 ]. The HGB systems are profuse and diverse, constitute one of the important geothermal zones in the world, and include some of the world’s largest thermal pools (e.g., Yangbajing geothermal field, South Western China).…”

Section: Introductionmentioning

confidence: 99%

High Variation in Protist Diversity and Community Composition in Surface Sediment of Hot Springs in Himalayan Geothermal Belt, China

et al. 2023

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Hot springs are some of the most special environments on Earth. Many prokaryotic and eukaryotic microbes have been found to live in this environment. The Himalayan geothermal belt (HGB) has numerous hot springs spread across the area. Comprehensive research using molecular techniques to investigate eukaryotic microorganisms is still lacking; investigating the composition and diversity of eukaryotic microorganisms such as protists in the hot spring ecosystems will not only provide critical information on the adaptations of protists to extreme conditions, but could also give valuable contributions to the global knowledge of biogeographic diversity. In this study, we used high-throughput sequencing to illuminate the diversity and composition pattern of protist communities in 41 geothermal springs across the HGB on the Tibetan Plateau. A total of 1238 amplicon sequence variants (ASVs) of protists were identified in the hot springs of the HGB. In general, Cercozoa was the phylum with the highest richness, and Bacillariophyta was the phylum with the highest relative abundance in protists. Based on the occurrence of protist ASVs, most of them are rare. A high variation in protist diversity was found in the hot springs of the HGB. The high variation in protist diversity may be due to the different in environmental conditions of these hot springs. Temperature, salinity, and pH are the most important environmental factors that affect the protist communities in the surface sediments of the hot springs in the HGB. In summary, this study provides the first comprehensive study of the composition and diversity of protists in the hot springs of the HGB and facilitates our understanding of the adaptation of protists in these extreme habitats.

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Unveiling the microbial diversity and functional dynamics of Shiv Kund, Sohna hot spring, India through a shotgun metagenomics approach

Saini,

Aamir,

Singh

et al. 2023

Arch Microbiol

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Bacterial diversity, physicochemical and geothermometry of South Asian hot springs

Cited by 8 publications

References 52 publications

Thermostable Amylase from Cytobacillus firmus: Characterization, Optimization, and Implications in Starch Hydrolysis

Thermostable Amylase from Cytobacillus firmus: Characterization, Optimization, and Implications in Starch Hydrolysis

High Variation in Protist Diversity and Community Composition in Surface Sediment of Hot Springs in Himalayan Geothermal Belt, China

Unveiling the microbial diversity and functional dynamics of Shiv Kund, Sohna hot spring, India through a shotgun metagenomics approach

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