Bacterial drug-resistance patterns and genetic diversity of bacteria-associated bacteriuria in diabetic patients in Ghana

Forson, Audrey; Menkah, Dickson Agyei; Quarchie, Marjorie Ntiwaa; Dhikrullahi, Shittu Bunkunmi; Michael, Olu-Taiwo; Codjoe, Francis S.

doi:10.1016/j.ijregi.2021.10.007

Cited by 16 publications

(11 citation statements)

References 49 publications

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“…Our data demonstrates high resistance rates of up to 100% to multiple standard antibiotics such as aminopenicillin +/− β-lactamase inhibitors, cephalosporines, or fluorochinolones, which, of course, must be distinguished from primary resistance. Of more immediate concern are the E. coli AMR rates against reserve antibiotics such as GEN and TET, which we report here to be at 61.7% (vs. 26.7% in Donkor et al [ 21 ] and 14.3% in Forson et al [ 19 ]) and 87.2% (vs. 26.7% in Donkor et al [ 21 ]), respectively. It must also be borne in mind that GEN is a purely intravenous antibiotic.…”

Section: Discussionmentioning

confidence: 58%

“…For each year, the odds increased by 3 per cent. In contrast, a study including 90 diabetic Ghanaian patients analysed by Forson et al in 2021 [ 19 ] demonstrated that the risk of bacterial growth decreased with increasing age (OR 0.417), and in the Bouza study [ 26 ], pregnancy emerged as the only clinical risk factor among study participants (OR 2.42). Sex, age, diabetes, history of UTI, and frequency of sex showed no significant association with UTI [ 26 ].…”

Section: Discussionmentioning

confidence: 97%

“…Studying a cohort of 90 Ghanaian diabetic patients with UTI, Forson et al found that K. spp. (55.6%) was the predominant uropathogen identified, with E. coli coming in second at 31.3% [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…Most frequently, E. coli (46.4%) was isolated, which displayed AMR rates against CIP of 38.5% [ 22 ]. Likewise, in the 2021 Forson study conducted at the Korle- Bu Hospital in Accra, only 21.4% of E. coli strains showed resistance to CIP [ 19 ]. Notably, in our cohort, the resistance rate of E. coli to CIP was 89.4%.…”

Section: Discussionmentioning

confidence: 99%

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The Antimicrobial Resistance (AMR) Rates of Uropathogens in a Rural Western African Area—A Retrospective Single-Center Study from Kpando, Ghana

et al. 2022

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Little is known about the antimicrobial resistance (AMR) status of uropathogens in Western Africa. We performed a retrospective evaluation of urine cultures collected from the rural Margret Marquart Catholic Hospital, Kpando, Ghana during the time period from October 2019–December 2021. Urine samples from 348 patients (median age 40 years, 52.6% male) were examined. Of these, 125 (35.9%) showed either fungal or bacterial growth, including Escherichia coli in 48 (38.4%), Candida species (spp.) in 29 (23.2%), Klebsiella spp. in 27 (21.6%), Proteus spp. in 12 (9.6%), Citrobacter spp. in 10 (8.0%), Salmonella spp. in 4 (3.2%), Staphylococcus spp. in 3 (2.4%), and Pseudomonas spp. in 2 (1.6%) cases. Two bacterial spp. were detected in 7 samples (5.6%). Antibiotic susceptibility testing showed resistance to a mean 8.6 out of 11 tested antibiotics per patient. Significant predictors (p < 0.05) of bacterial growth were age (OR 1.03), female sex (OR 3.84), and the number of pus cells (OR 1.05) and epithelial cells (OR 1.07) in urine microscopy. We observed an alarmingly high AMR rate among the uropathogens detected, even to reserve antibiotics. A similar resistance profile can be expected in West African patients living in high-income countries. These observations warrant the implementation of restrictive antibiotic protocols, together with the expansion of urine culture testing capacities, improvement of documentation and reporting of AMR rates, and continued research and development of new antibiotic therapies in order to stem the progression of AMR in this West African region.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The Antimicrobial Resistance (AMR) Rates of Uropathogens in a Rural Western African Area—A Retrospective Single-Center Study from Kpando, Ghana

et al. 2022

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“…Complementar a Forson et al (2021), no estudo de Gravey . (2017) se verificou que dos 33.302 exames de pacientes acima de 65 anos, foram identificados 13.450 microrganismos.…”

Section: Prevalência E Resistência Bacterianaunclassified

Análise Epidemiológica Da Incidência Do Câncer De Estômago No Pará No Triênio 2019-2021

Júnior¹,

Silva²

2022

O Que as Pesquisas Revelam Sobre a Saúde No Brasil

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Os autores se responsabilizam publicamente pelo conteúdo desta obra, garantindo que o mesmo é de autoria própria, assumindo integral responsabilidade diante de terceiros, quer de natureza moral ou patrimonial, em razão de seu conteúdo, declarando que o trabalho é original, livre de plágio e que não infringe quaisquer direitos de propriedade intelectual de terceiros. Os autores declaram não haver qualquer interesse comercial ou irregularidade que comprometa a integridade desta obra.

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Uropathogens and their antibiotic susceptibility patterns among diabetic patients at st. john of god hospital, duayaw nkwanta, Ghana: a cross‐sectional study

Iddrisu,

Owusu,

Doe

et al. 2024

Health Science Reports

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BackgroundUropathogens are microorganisms that cause urinary tract infections (UTIs). Owing to higher blood glucose levels and compromised immune functions, treatment of uropathogens in diabetic patients is a challenge.AimThis study aims to assess the prevalence of uropathogens and their antibiotic susceptibility among diabetic patients at St. John of God Hospital at Duayaw Nkwanta (SJGHDN) in the Ahafo region of Ghana.MethodsThe cross‐sectional study recruited 175 diabetic patients at SJGHDN between August and September 2023. Questionnaires were used to collect patients’ background information. Fasting Blood Sugar (FBS) was assessed by using a glucometer. Urine samples were examined for the presence of uropathogens. A sterile inoculating loop with a calibrated volume of 2 µl was used for plating. Each colony equals 500 CFU/mL. Significant uropathogen was determined by multiplying the counted colonies by 500 to obtain CFU/mL. Positive uropathogen was defined as CFU ≥ 105/mL. Significant uropathogen was defined as ≥200 colonies per sample. The disc diffusion method was used to determine antibiotic susceptibility.ResultsOut of the 175 patients, 19.4% expressed various uropathogens with Escherichia coli being the predominant. Suboptimal glucose level was the most significant risk factor (p = 0.038). Glucosuria (p = 0.036), hazy urine (p = 0.028), positive leukocyte esterase (p = 0.001), and pus cells in urine sediment (p = 0.020) were significant indicators of uropathogen occurrence. Klebsiella pneumonia and Proteus mirabilis were resistant to ≥4 antibiotics. Amikacin, nitrofurantoin, levofloxacin, ciprofloxacin, and ceftriaxone demonstrated efficacy against the isolates.ConclusionThis study underscores the notable prevalence of uropathogens in diabetic patients and the alarming levels of antibiotic resistance observed. The results highlight the critical need for vigilant monitoring and customized treatment approaches, particularly for diabetic patients exhibiting risk factors such as elevated urine glucose levels, cloudy urine, and presence of leukocyte esterase and pus cells in urine sediment. The significant resistance to frequently used antibiotics like co‐trimoxazole and tetracycline points to the necessity of routine susceptibility testing and the use of alternative antibiotics for effective treatment. These findings can assist healthcare providers in more effectively managing and preventing UTIs in diabetic populations.

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Bacterial drug-resistance patterns and genetic diversity of bacteria-associated bacteriuria in diabetic patients in Ghana

Cited by 16 publications

References 49 publications

The Antimicrobial Resistance (AMR) Rates of Uropathogens in a Rural Western African Area—A Retrospective Single-Center Study from Kpando, Ghana

The Antimicrobial Resistance (AMR) Rates of Uropathogens in a Rural Western African Area—A Retrospective Single-Center Study from Kpando, Ghana

Análise Epidemiológica Da Incidência Do Câncer De Estômago No Pará No Triênio 2019-2021

Uropathogens and their antibiotic susceptibility patterns among diabetic patients at st. john of god hospital, duayaw nkwanta, Ghana: a cross‐sectional study

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