Bacterial dynamics of sugarcane silage in the tropics

Abstract

The objective of this study was to evaluate changes in the bacterial community in sugarcane silage, in distinct soil types along the storage period. We depicted the bacterial community associated with sugarcane, before and after ensiling, through a massive sequencing of the gene 16S rRNA using MiSeq platform. The ensilage process shifted the composition of the bacterial community from the heterofermentative lactic acid bacteria Leuconostoc to bacteria belonging to the genera Acinetobacter, Ralstonia and Novosphingobium. However, this shift did not convey statically significant differences in alfa diversity metrics. In addition, similarity percentage analysis showed that the bacterial Operational Taxonomic Units that were primarily responsible for the observed differences were Leuconostoc, Pseudomonas, Acinetobacter, Ralstonia, Fructobacillus, Novosphingobium, Lactobacillus, Burkholderia and Clostridium sensu stricto 1. The storage period was the most important factor responsible for changes in the bacterial community of silages. Results confirmed that the type of soil did not influence the dissimilarity found among samples.

Time of Day for Harvest Affects the Fermentation Parameters, Bacterial Community, and Metabolic Characteristics of Sorghum-Sudangrass Hybrid Silage

“… Acinetobacter species are aerobic, nonfermenting bacteria that can be found in different environments. Some Acinetobacter species can survive in anaerobic environment in the presence of acetate as a substrate ( 48 ). They were reported to link the aerobic deterioration ( 49 ) and DM losses ( 50 ) in silages.…”

Effects of Malic Acid and Sucrose on the Fermentation Parameters, CNCPS Nitrogen Fractions, and Bacterial Community of Moringa oleifera Leaves Silage

“…Actually, Leuconostoc is regarded as an early colonizer and has poor tolerance to low pH conditions. As fermentation progresses, Leuconostoc is gradually replaced by acid-tolerant LABs, such as Lactobacillus [ 58 ]. As for other genera, Pannonibacter and Inhella have been reported to exert the effect of biological denitrification and reduce nitrate to nitrite [ 59 , 60 ]; Caulobacter plays functional roles in enhancing plant growth by synthesizing siderophores, solubilizing phosphate and producing indole-3-acetic acid [ 61 ].…”

First characterization of the probiotic potential of lactic acid bacteria isolated from Costa Rican pineapple silages

“…However, resistance for all the isolates was higher when compared with the control. It is hypothesized that the tolerance to acidic conditions observed in this study may be related to the ensilage process, in which the LAB that survive the last stages were subjected to acidic pH for a prolonged period of time ( Muraro et al., 2021 ). Besides, these results indicate that some of the isolates may be able to survive the normal gastric environment.…”