Bacterial endophyte communities in Pinus flexilis are structured by host age, tissue type, and environmental factors

Carper, Dana L.; Carrell, Alyssa A.; Kueppers, Lara M.; Frank, A. Carolin

doi:10.1007/s11104-018-3682-x

Cited by 36 publications

(29 citation statements)

References 100 publications

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“…The plant organ (leaves vs. twigs) was found to significantly affect the composition of bacterial communities in the olive tree phyllosphere, as reported in previous studies for other plant species, like Coffea arabica [65], Pinus flexilis [9] or Populus [68]. This effect was greater within the epiphytic than within the endophytic bacterial communities, which could be related with the greater differences between leaves and twigs on their surfaces, when compared with the internal plant tissues.…”

Section: Discussionsupporting

confidence: 78%

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Epiphytic and Endophytic Bacteria on Olive Tree Phyllosphere: Exploring Tissue and Cultivar Effect

et al. 2020

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Variation on bacterial communities living in the phyllosphere as epiphytes and endophytes has been attributed to plant host effects. However, there is contradictory or inconclusive evidence regarding the effect of plant genetics (below the species' level) and of plant tissue type on phyllosphere bacterial community assembly, in particular when epiphytes and endophytes are considered simultaneously. Here, both surface and internal bacterial communities of two olive (Olea europaea) cultivars were evaluated in twigs and leaves by molecular identification of cultivable isolates, with an attempt to answer these questions. Overall, Proteobacteria, Actinobacteria and Firmicutes were the dominant phyla, being epiphytes more diverse and abundant than endophytes. Host genotype (at cultivar level) had a structuring effect on the composition of bacterial communities and, in a similar way, for both epiphytes and endophytes. Plant organ (leaf vs. twig) control of the bacterial communities was less evident when compared with plant genotype and with a greater influence on epiphytic than on endophytic community structure. Each olive genotype/plant organ was apparently selective towards specific bacterial operational taxonomic units (OTUs), which may lead to specific feedbacks on fitness of plant genotypes. Bacterial recruitment was observed to happen mainly within epiphytes than in endophytes and in leaves as compared with twigs. Such host specificity suggested that the benefits derived from the plantbacteria interaction should be considered at genetic levels below the species.

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Section: Discussionsupporting

confidence: 78%

“…Previous studies have demonstrated that different environmental and plant-dependent factors, such as host species and plant organ, contribute to the shaping of bacterial communities in the phyllosphere [7][8][9]. Most of these studies have focused on those bacteria associated to the phyllosphere of specific host species [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Epiphytic and Endophytic Bacteria on Olive Tree Phyllosphere: Exploring Tissue and Cultivar Effect

et al. 2020

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“…These isolates have also shown nitrogen fixation ability, as well as ability to solubilize inorganic and organic phosphate (Given et al, unpublished), which could benefit the plants in the nutrient poor soils -in the experimental site soil contains very little soluble nitrate (NO − 3 ), ammonium (NH + 4 ), or phosphate (PO 4 ) (Kumar et al, 2016). Intriguingly, Oxalobacteraceae have been also reported as a major endophyte group in Pinus flexilis seedlings in nutrient poor soils (Carper et al, 2018).…”

Section: Discussionmentioning

confidence: 96%

“…The available studies on the succession of phyllosphere microbiome (Copeland et al, 2015), rhizosphere microbiome (Chaparro et al, 2014), and root endophytic communities (Yuan et al, 2015) focus on agricultural plants in temperate climate. In contrast, only a few studies have focused on wild plants in high-stress environments like the Arctic, alpine treelines, or deserts (Nissinen et al, 2012;Coleman-Derr et al, 2016;Kumar et al, 2017a;Carper et al, 2018), and no data is currently available on plant colonization by microbes in these biomes.…”

Section: Introductionmentioning

confidence: 99%

Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna

et al. 2020

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The rapid developments in the next-generation sequencing methods in the recent years have provided a wealth of information on the community structures and functions of endophytic bacteria. However, the assembly processes of these communities in different plant tissues are still currently poorly understood, especially in wild plants in natural settings. The aim of this study was to compare the composition of endophytic bacterial communities in leaves and roots of arcto-alpine pioneer plant Oxyria digyna, and investigate, how plant tissue (leaf or root) or plant origin affect the community assembly. To address this, we planted micropropagated O. digyna plants with low bacterial load (bait plants) in experimental site with native O. digyna population, in the Low Arctic. The endophytic bacterial community structures in the leaves and roots of the bait plants were analyzed after one growing season and one year in the field, and compared to those of the wild plants growing at the same site. 16S rRNA gene targeted sequencing revealed that endophytic communities in the roots were more diverse than in the leaves, and the diversity in the bait plants increased in the field, and was highest in the wild plants. Both tissue type and plant group had strong impact on the endophytic bacterial community structures. Firmicutes were highly abundant in the leaf communities of both plant types. Proteobacteria and Bacteroidetes were more abundant in the roots, albeit with different relative abundances in different plant groups. The community structures in the bait plants changed in the field over time, and increasingly resembled the wild plant endophytic communities. This was due to the changes in the relative abundances of several bacterial taxa, as well as species acquisition in the field, but with no species turnover. Several OTUs that were acquired by the bait plants in the field and represent phosphate solubilizing and diazotrophic bacterial taxa, suggesting major role in nutrient acquisition of these bacteria for this nonmycorrhizal plant, thriving in the nutrient poor arctic soils.

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“…However, several methodological obstacles arise when studying endophytes, including the complete removal of leaf surface microorganisms (i.e., leaf epiphytes). Current methods used to confirm leaf surface sterility generally include polymerase chain reaction (PCR) and leaf prints on nutrient media [10][11][12][13][14][15][16][17][18]. We included scanning electronic microscopy (SEM) to improve the validation of leaf surface sterility and to evaluate the potential damage by these treatments on the integrity of leaf tissue.…”

Section: Introductionmentioning

confidence: 99%

Simple methods to remove microbes from leaf surfaces

2020

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Bacterial endophyte communities in Pinus flexilis are structured by host age, tissue type, and environmental factors

Cited by 36 publications

References 100 publications

Epiphytic and Endophytic Bacteria on Olive Tree Phyllosphere: Exploring Tissue and Cultivar Effect

Epiphytic and Endophytic Bacteria on Olive Tree Phyllosphere: Exploring Tissue and Cultivar Effect

Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant Oxyria digyna

Simple methods to remove microbes from leaf surfaces

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