Bacterial endophytes of sweet potato tuberous roots affected by the plant genotype and growth stage

Marques, Joana Montezano; Silva, Thais Freitas da; Vollú, Renata Estebanez; Mateus, Jackeline Rossetti; Blank, Arie Fitzgerald; Smalla, Kornelia; Seldin, Lucy

doi:10.1016/j.apsoil.2015.08.020

Cited by 65 publications

(55 citation statements)

References 36 publications

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“…Given the worldwide nutritional value of sweetpotatoes, it is desirable to explore the potential of the microbiome to promote sweetpotato yield, drought tolerance, and to manage sweetpotato pests and disease. To date, culture-independent and -dependent techniques have elucidated the impact of some sweetpotato genotypes on sweetpotato rhizosphere microbiome composition (Marques et al 2014) and produced isolated microorganisms in culture from the sweetpotato endophyte community (Marques et al 2015) . Rhizosphere versus surrounding soil contained different microbial community composition in three sweetpotato genotypes (Marques et al 2014) .…”

Section: Introductionmentioning

confidence: 99%

Surveying the sweetpotato rhizosphere, endophyte, and surrounding soil microbiomes at two North Carolina farms reveals underpinnings of sweetpotato microbiome community assembly

Pepe-Ranney

Keyser

Trimble

et al. 2019

Preprint

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Farmers grow sweetpotatoes worldwide and some sub-Saharan African and Asian diets include sweetpotato as a staple, yet the sweetpotato microbiome is conspicuously less studied relative to crops such as maize, soybean, and wheat. Studying sweetpotato microbiome ecology may reveal paths to engineer the microbiome to improve sweetpotato yield, and/or combat sweetpotato pests and diseases. We sampled sweetpotatoes and surrounding soil from two North Carolina farms. We took samples from sweetpotato fields under two different land management regimes, conventional and organic, and collected two sweetpotato cultivars,

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Section: Introductionmentioning

confidence: 99%

Surveying the sweetpotato rhizosphere, endophyte, and surrounding soil microbiomes at two North Carolina farms reveals underpinnings of sweetpotato microbiome community assembly

Pepe-Ranney

Keyser

Trimble

et al. 2019

Preprint

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“…Interestingly, the plant genotype may also affect the functional diversity of endophytic bacteria as IAA-producing strains were predominantly isolated from one of the three genotypes of sweet potato studied (Marques et al, 2015).…”

Section: Factors Driving Endophytic Bacterial Communitiesmentioning

confidence: 99%

“…Endophytic bacterial microbiomes may vary according to host plant species (Ding & Melcher, 2016;Shen & Fulthorpe, 2015), plant genotype (Marques et al, 2015;Rodriguez-Blanco et al, 2015), plant organs (Hameed et al, 2015), plant developmental stage (e.g. seedling or mature plant) (de Almeida Lopes et al, 2016;Ren et al, 2015a;Yu et al, 2015), growing season (e.g.…”

Section: Factors Driving Endophytic Bacterial Communitiesmentioning

confidence: 99%

“…Gammaproteobacteria of the genera Enterobacter and Pseudomonas are the dominant endophytic bacteria on different plants, including tuberous roots of sweet potato (Marques et al, 2015), rice roots (Ferrando & Scavino, 2015;Ren et al, 2015a;Sessitsch et al, 2012), and the roots of mature trees of Populus deltoids (Gottel et al, 2011). The predominance of one or two operational taxonomic units (OTUs) in specific plant tissues has been reported after endophytic bacterial sequencing, such as a pseudomonas-like OTU in the roots of P. deltoids (Gottel et al, 2011) and two OTUs affiliated to Pseudomonas and Enterobacter in sugarcane stems (Magnani et al 2013).…”

Section: Biodiversity Of Endophytic Bacteriamentioning

confidence: 99%

“…The chemical disinfection seems to be more effective but may underestimate the presence of bacteria as it damages DNA (Reinhold-Hurek et al, 2015). A diverse range of bacteria have been isolated from surface-sterilized plant tissues, such as roots, tubers, stems, leaves, seeds, flowers, fruits and legume nodules (Compant et al, 2011;Deng et al, 2011;Marques et al, 2015;Rosenblueth & Martínez-Romero, 2006;Truyens et al, 2015). These isolates are considered to be endophytes and include representatives of the Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes (Rosenblueth & Martínez-Romero, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Effects of strategic tillage and plant hormone treatments on wheat-associated microbial communities

Liu¹

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The main aim of this thesis was to characterise microbial properties of microbiomes associated with wheat (Triticum aestivum) soils and wheat plants. In particular my objective was to better understand the effects on microbial communities following strategic tillage (ST) in wheat field soils and the activation of plant defence pathways in wheat plants. Throughout the thesis, multiple culture-independent methods, especially next-generation sequencing of 16S rRNA gene were used to profile soil microbial communities. Over half of the arable land in the northern grains region of Australia is managed using no-till (NT), a farming method which has improved crop yields and soil quality while reducing the input and labour costs. However, in recent years, concerns have arisen among farmers over the weed control in continuous NT systems. Strategic tillage has been touted as a potential solution, in particular for the severe weed infestations of long-term NT. Nevertheless, there is little information on the influence of ST on the microbial properties of Australian NT soils.In the present study, ST applications and soil sampling were performed in Moonie, Moree and Condamine during the fallow period in eastern Australia. These sites were chosen based on their long history of repeated wheat cultivation under NT farming practices and their different soil types.Overall, results show that in the Moonie trial on a Calcisol, one-time ST with either chisel or offset disc did not significantly influence the composition of soil bacterial communities when measured 13 months after tillage. However, relative to the NT, chisel tillage led to significant increases in microbial biomass carbon (MBC), abundances of Alphaproteobacteria, Bacteroidetes and Firmicutes as well as the utilisation of D+cellubiose and mannitol at 0-10 cm depth. In the Moree trial on a grey Vertosol, ST with different timing and implement demonstrated great potential in weed control and did not influence wheat yield and soil physicochemical and biological properties in the short-term. In the Condamine site on a Solonetz soil, one-or two-time chisel tillage did not influence soil MBC, total microbial enzymatic activity (MEA) or utilisation of C substrates.Likewise, ST did not change the soil microbial community structure and the abundance of genes encoding enzymes involved in key steps of C and N reactions. However, one-time chisel increased relative abundance of Acidobacteria RB41 and Acidobacteria iii1-15, and two-time chisel slightly increased the average C utilisation, at 10-20 cm depth. Overall, this thesis suggests that ST does not cause major impacts on soil properties of long-term NT and could be potentially used to address the long-term NT-associated issues without impacting overall soil properties.Recent reports demonstrate the importance of microbiomes associated with plants and their soil they are cultivated in. Beneficial microbes can significantly increase crop yields and provide biocontrol functions against plant pathogens, but prior to this stu...

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16S rRNA gene‐based identification and plant growth‐promoting potential of cultivable endophytic bacteria

Raimi

Adeleke

2023

Agronomy Journal

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Endophytic bacteria play essential roles in plant growth and fitness through nitrogen fixation, nutrient solubilization, and phytohormone production. However, the plant growth-promoting (PGP) potential of endophytic microorganisms inhabiting vegetables is underexplored. Thus, this study investigated the endophytic bacteria of vegetable crops for their PGP properties using the culture-dependent technique. Recovered isolates were identified by sequencing the partial 16S rRNA gene using Sanger sequencing. The isolated endophytic bacteria belonged to 15 genera and 39 different species. Bacillus, Enterobacter, Pantoea, and Pseudomonas were the predominant genera and had high phosphate solubilization and siderophore production potential. The isolates differentially produced indole-3-acetic acid and ammonium in the range 0.14-44.48 μg mL -1 and 0.77-93.24 mg L -1 , respectively. Pseudomonas azotoformans, Stenotrophomonas maltophilia, and Serratia liquefaciens showed a high level of siderophore production, which was not significantly (p > 0.05) different across the plant and organ types. More than 21% of endophytes could produce gelatinase, protease, amylase, and cellulase, contributing to their diverse ecosystem functions. Bacterial density and species composition varied across the vegetable species, organs, and farm locations but were higher in the stems compared with other organs. However, the vegetable organ had no significant (p > 0.05) influence on the bacterial population density, except for the interaction of plant species and organs.Overall, this study provides basic knowledge about the cultivable endophytic bacterial community of vegetables and reveals diverse endophytic bacteria useful in bioinoculant formulations for improving crop productivity.

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Bacterial endophytes of sweet potato tuberous roots affected by the plant genotype and growth stage

Cited by 65 publications

References 36 publications

Surveying the sweetpotato rhizosphere, endophyte, and surrounding soil microbiomes at two North Carolina farms reveals underpinnings of sweetpotato microbiome community assembly

Surveying the sweetpotato rhizosphere, endophyte, and surrounding soil microbiomes at two North Carolina farms reveals underpinnings of sweetpotato microbiome community assembly

Effects of strategic tillage and plant hormone treatments on wheat-associated microbial communities

16S rRNA gene‐based identification and plant growth‐promoting potential of cultivable endophytic bacteria

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