Bacterial endophytome-mediated resistance in banana for the management of Fusarium wilt

Nakkeeran, S.; Rajamanickam, Subapriya; Saravanan, R.; Vanthana, M; Soorianathasundaram, K.

doi:10.1007/s13205-021-02833-5

Cited by 37 publications

(21 citation statements)

References 76 publications

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“…In recent years, the research of WRKY and MAPK genes has attracted much attention. Many studies have been conducted on the respective functions of WRKY and MAPK genes and their interactions [36][37][38][39]. All these studies provide references and ideas for our research.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression and Interaction Analysis of FsWRKY4 and FsMAPK3 in Forsythia suspensa

Tan,

Chen,

Zhang

et al. 2023

Plants

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Forsythia suspensa is a deciduous shrub that belongs to the family Myrtaceae, and its dried fruits are used as medicine. F. suspensa contains several secondary metabolites, which exert pharmacological effects. One of the main active components is forsythin, which exhibits free radical scavenging, antioxidant, anti-inflammatory, and anti-cancer effects. Mitogen-activated protein kinase (MAPKs) can increase the activity of WRKY family transcription factors in a phosphorylated manner, thereby increasing the content of secondary metabolites. However, the mechanism of interaction between MAPKs and WRKYs in F. suspensa remains unclear. In this study, we cloned the genes of FsWRKY4 and FsMAPK3, and performed a bioinformatics analysis. The expression patterns of FsWRKY4 and FsMAPK3 were analyzed in the different developmental stages of leaf and fruit from F. suspensa using real-time fluorescence quantitative PCR (qRT-PCR). Subcellular localization analysis of FsWRKY4 and FsMAPK3 proteins was performed using a laser scanning confocal microscope. The existence of interactions between FsWRKY4 and FsMPAK3 in vitro was verified by yeast two-hybridization. Results showed that the cDNA of FsWRKY4 (GenBank number: OR566682) and FsMAPK3 (GenBank number: OR566683) were 1587 and 522 bp, respectively. The expression of FsWRKY4 was higher in the leaves than in fruits, and the expression of FsMAPK3 was higher in fruits but lower in leaves. The subcellular localization results indicated that FsWRKY4 was localized in the nucleus and FsMAPK3 in the cytoplasm and nucleus. The prey vector pGADT7-FsWRKY4 and bait vector pGBKT7-FsMAPK3 were constructed and co-transferred into Y2H Glod yeast receptor cells. The results indicated that FsWRKY4 and FsMAPK3 proteins interact with each other in vitro. The preliminary study may provide a basis for more precise elucidation of the synthesis of secondary metabolites in F. suspensa.

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Section: Discussionmentioning

confidence: 99%

Gene Expression and Interaction Analysis of FsWRKY4 and FsMAPK3 in Forsythia suspensa

Tan,

Chen,

Zhang

et al. 2023

Plants

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“…vesicatoria , Erwinia carotovora subsp. carotovora ) in pepper, cucumber, banana, and Arabidopsis thaliana ( Sang et al, 2014 ; Nakkeeran et al, 2021 ; Yadav M. et al, 2021 ). Nine genes involved in ISR were explored in P. polymyxa , with higher sequence identity (> 95%) in different strains, while key genes associated with volatile organic compounds (2,3-butanediol, methanethiol and isoprene) were contained ( Li et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative genomic and functional analyses of Paenibacillus peoriae ZBSF16 with biocontrol potential against grapevine diseases, provide insights into its genes related to plant growth-promoting and biocontrol mechanisms

Yuan

Jiang

et al. 2022

Front. Microbiol.

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Paenibacillus peoriae is a plant growth-promoting rhizobacteria (PGPR) widely distributed in various environments. P. peoriae ZBFS16 was isolated from the wheat rhizosphere and significantly suppressed grape white rot disease caused by Coniella vitis. Here, we present the complete genome sequence of P. peoriae ZBFS16, which consists of a 5.83 Mb circular chromosome with an average G + C content of 45.62%. Phylogenetic analyses showed that ZBFS16 belongs to the genus P. peoriae and was similar to P. peoriae ZF390, P. peoriae HS311 and P. peoriae HJ-2. Comparative analysis with three closely related sequenced strains of P. peoriae identified the conservation of genes involved in indole-3-acetic acid production, phosphate solubilization, nitrogen fixation, biofilm formation, flagella and chemotaxis, quorum-sensing systems, two-component systems, antimicrobial substances and resistance inducers. Meanwhile, in vitro experiments were also performed to confirm these functions. In addition, the strong colonization ability of P. peoriae ZBFS16 was observed in soil, which provides it with great potential for use in agriculture as a PGPR. This study will be helpful for further studies of P. peoriae on the mechanisms of plant growth promotion and biocontrol.

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“…1 ). Strains from the genera Bacillus , Pantoea , and Enterobacter were known to exhibit antagonistic behavior against Fusarium wilt ( 9 ), which is one of the most harmful diseases affecting banana plants. The data on whole-genome sequencing presented in this study can aid in the identification of genes related to endophyte-mediated resistance in bananas and contribute toward the management of Fusarium wilt.…”

Section: Announcementmentioning

confidence: 99%

Endophytic bacteria associated with wild-type banana seed ( Musa balbisiana ): whole genome sequencing

Kumar,

Gan,

Popielarz

et al. 2023

Microbiol Resour Announc

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We present the whole-genome sequences of five endophytic bacteria isolated from Musa balbisiana seeds. These strains represent five different genera: Bacillus, Brachybacterium, Enterobacter, Enterococcus, and Pantoea . Among these, three genera ( Bacillus , Pantoea , and Enterobacter ) were previously recognized for their antagonistic effects against Fusarium wilt, a highly destructive disease that affects banana plants.

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Bacterial endophytome-mediated resistance in banana for the management of Fusarium wilt

Cited by 37 publications

References 76 publications

Gene Expression and Interaction Analysis of FsWRKY4 and FsMAPK3 in Forsythia suspensa

Gene Expression and Interaction Analysis of FsWRKY4 and FsMAPK3 in Forsythia suspensa

Comparative genomic and functional analyses of Paenibacillus peoriae ZBSF16 with biocontrol potential against grapevine diseases, provide insights into its genes related to plant growth-promoting and biocontrol mechanisms

Endophytic bacteria associated with wild-type banana seed ( Musa balbisiana ): whole genome sequencing

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