Bacterial exopolysaccharides for improvement of technological, functional and rheological properties of yoghurt

Tiwari, Swati; Kavitake, Digambar; Devi, Palanisamy Bruntha; Shetty, Prathapkumar Halady

doi:10.1016/j.ijbiomac.2021.05.140

Cited by 90 publications

(60 citation statements)

References 87 publications

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“…The ability of FucoPol to form emulsion with olive oil was not affected, with the emulsions prepared with the sample recovered with the optimized procedure displaying the same E24 value (98%). Moreover, the resulting emulsions were characterized by a significantly higher apparent viscosity, a feature of relevance for FucoPol’s utilization in cosmetic, pharmaceutical or food products [ 1 , 2 , 5 , 8 , 9 , 55 , 56 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

“…Given their extracellular nature, EPS recovery from the culture broth involves rather simple downstream procedures that usually consist of cell removal, followed by biopolymer precipitation from the cell-free supernatant by addition of a precipitating agent (e.g., methanol, ethanol, isopropanol, acetone) and drying [ 1 , 8 , 9 ]. Although solvent precipitation with ethanol or acetone is the prevalent technique for EPS extraction, it usually yields rather impure polymers with high salts and/or protein contents [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties

et al. 2022

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In this study, membrane-based methods were evaluated for the recovery of FucoPol, the fucose-rich exopolysaccharide (EPS) secreted by the bacterium Enterobacter A47, aiming at reducing the total water consumption and extraction time, while keeping a high product recovery, thus making the downstream procedure more sustainable and cost-effective. The optimized method involved ultrafiltration of the cell-free supernatant using a 30 kDa molecular weight cut-off (MWCO) membrane that allowed for a 37% reduction of the total water consumption and a 55% reduction of the extraction time, compared to the previously used method (diafiltration-ultrafiltration with a 100 kDa MWCO membrane). This change in the downstream procedure improved the product’s recovery (around 10% increase) and its purity, evidenced by the lower protein (8.2 wt%) and inorganic salts (4.0 wt%) contents of the samples (compared to 9.3 and 8.6 wt%, respectively, for the previously used method), without impacting FucoPol’s sugar and acyl groups composition, molecular mass distribution or thermal degradation profile. The biopolymer’s emulsion-forming and stabilizing capacity was also not affected (emulsification activity (EA) with olive oil, at a 2:3 ratio, of 98 ± 0% for all samples), while the rheological properties were improved (the zero-shear viscosity increased from 8.89 ± 0.62 Pa·s to 17.40 ± 0.04 Pa·s), which can be assigned to the higher purity degree of the extracted samples. These findings demonstrate a significant improvement in the downstream procedure raising FucoPol’s recovery, while reducing water consumption and operation time, key criteria in terms of process economic and environmental sustainability. Moreover, those changes improved the biopolymer’s rheological properties, known to significantly impact FucoPol’s utilization in cosmetic, pharmaceutical or food products.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties

et al. 2022

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“…bulgaricus and Streptococcus thermophilus , were selected to produce exopolysaccharides, generally 60–150 mg/L and 30–890 mg/L, respectively [ 8 , 124 ]. In recent years, studies have been conducted on new EPS-producing starter or adjunct cultures to be used in yoghourt production [ 8 , 125 ]. For example, Han et al [ 126 ] evaluated 19 high EPS-producing Str.…”

Section: Application Of Eps-producing Lab In Food Productsmentioning

confidence: 99%

Exopolysaccharides Produced by Lactic Acid Bacteria: From Biosynthesis to Health-Promoting Properties

Jurášková

Ribeiro

Silva

2022

Foods

149

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The production of exopolysaccharides (EPS) by lactic acid bacteria (LAB) has attracted particular interest in the food industry. EPS can be considered as natural biothickeners as they are produced in situ by LAB and improve the rheological properties of fermented foods. Moreover, much research has been conducted on the beneficial effects of EPS produced by LAB on modulating the gut microbiome and promoting health. The EPS, which varies widely in composition and structure, may have diverse health effects, such as glycemic control, calcium and magnesium absorption, cholesterol-lowering, anticarcinogenic, immunomodulatory, and antioxidant effects. In this article, the latest advances on structure, biosynthesis, and physicochemical properties of LAB-derived EPS are described in detail. This is followed by a summary of up-to-date methods used to detect, characterize and elucidate the structure of EPS produced by LAB. In addition, current strategies on the use of LAB-produced EPS in food products have been discussed, focusing on beneficial applications in dairy products, gluten-free bakery products, and low-fat meat products, as they positively influence the consistency, stability, and quality of the final product. Highlighting is also placed on reports of health-promoting effects, with particular emphasis on prebiotic, immunomodulatory, antioxidant, cholesterol-lowering, anti-biofilm, antimicrobial, anticancer, and drug-delivery activities.

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“…For instance, Lactobacillus helveticus MB2-1 isolated from traditional Sayram ropy fermented milk (SRFM) synthesized a HePS composed of galactose, glucose and mannose and it exhibited strong scavenging activities on three kinds of radicals ( Li et al, 2014a ). Moreover, several physio-technical characteristics of EPS have been investigated and extensive efforts have been put forth to explore its structural–functional relationships ( Korcz and Varga, 2021 , Tiwari et al, 2021 ). Recent developments in the field of EPSs have resulted in a renewed interest in the structure and biosynthesis mechanism of EPSs.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir

Xiao

Tang

et al. 2021

Food Chemistry: Molecular Sciences

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Bacterial exopolysaccharides for improvement of technological, functional and rheological properties of yoghurt

Cited by 90 publications

References 87 publications

Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties

Extraction of the Bacterial Extracellular Polysaccharide FucoPol by Membrane-Based Methods: Efficiency and Impact on Biopolymer Properties

Exopolysaccharides Produced by Lactic Acid Bacteria: From Biosynthesis to Health-Promoting Properties

Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir

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