Bacterial Flora of Respiratory Tract in Patients with Cystic Fibrosis, 1950-1971

Mearns, Margaret B.; Hunt, G. H.; Rushworth, R. Louise

doi:10.1136/adc.47.256.902

Cited by 88 publications

(42 citation statements)

References 7 publications

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“…The present study also identified higher resistance to other antibiotics, including tobramycin (51.6%), piperacillin-tazobactam, gatifloxacin, netilmicin (44.2%), ciprofloxacin and gentamicin (35.9%). However, resistance to cefoperazone and cefepime was detected in 28.9% of samples, which is similar to other studies demonstrating reduced susceptibility to commonly used antibiotics (3,4,11,12).…”

Section: Discussionsupporting

confidence: 75%

“…Cystic fibrosis (CF) is an autosomal recessive genetic disease (6); the opportunistic pathogen Pseudomonas aeruginosa may cause chronic lung disease in CF patients, depending on the genetic adaptation of the pathogen, and is a prevalent pathogen in CF patients with pulmonary infection (1)(2)(3)(4)(5). Worldwide, 80% of CF patients were found to be infected with P. aeruginosa (7,8).…”

Section: Introductionmentioning

confidence: 99%

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Characterization by phenotypic and genotypic methods of metallo-β-lactamase-producing Pseudomonas aeruginosa isolated from patients with cystic fibrosis

Zhang

Wang

et al. 2014

Molecular Medicine Reports

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Abstract.Pseudomonas aeruginosa continues to be a predominant cause of infections with high intrinsic resistance to antibiotics, resulting in treatment failure. P. aeruginosa is the leading cause of respiratory infections among cystic fibrosis (CF) patients. Resistance to carbapenem antibiotics among P. aeruginosa has been reported. Thus, this study was undertaken to characterize the metallo-β-lactamase (MBL) production of P. aeruginosa by phenotypic and genotypic methods. A total of 572 sputum samples were collected from cystic fibrosis patients along with the patient demographic details in a questionnaire. In total, 217 P. aeruginosa isolates were collected and an antibiogram revealed that 159 (73.3%) and 141 (64.9%) of these colonies exhibited resistance to imipenem and meropenem, respectively. Ceftazidime and tobramycin resistance were both identified in 112 (51.6%) isolates, and resistance to piperacillin-tazobactam, gatifloxacin and netilmicin was detected in 96 (44.2%) respective samples. A total of 62 (28.6%) respective samples were resistant to cefoperazone, cefepime and ceftriaxone. The least antibiotic resistance was shown to amikacin and ceftizoxime with 51 (23.5%) and 32 (14.7%) respective colonies resistant to the antibiotics. The minimum inhibitory concentration (MIC) for imipenem revealed a reduction in the MIC values. MBL screening by the zone enhancement method using ceftazidime plus EDTA discs demonstrated that 63 (56.25%) of the colonies were positive for MBL. A total of 53 (84.1%) samples expressed blaVIM and 48 (76.1%) expressed blaIMP genes, as detected by duplex polymerase chain reaction. In conclusion, carbapenem resistance is of great clinical concern in cystic fibrosis patients with P. aeruginosa infection. Therefore, mandatory regular screening and monitoring the resistance in P. aeruginosa among CF patients is required.

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Section: Discussionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Characterization by phenotypic and genotypic methods of metallo-β-lactamase-producing Pseudomonas aeruginosa isolated from patients with cystic fibrosis

Zhang

Wang

et al. 2014

Molecular Medicine Reports

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“…KEYWORDS: Cystic fibrosis, epidemiology, genotyping, national survey, Pseudomonas aeruginosa P seudomonas aeruginosa, widely spread in soils and water, has been the leading pathogen in cystic fibrosis (CF) lung pathology since the 1970s [1][2][3]. The means by which this organism is acquired are not yet fully elucidated.…”

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confidence: 99%

Survey ofPseudomonas aeruginosagenotypes in colonised cystic fibrosis patients

Daele¹

2006

Eur Respir J

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The current authors aimed to examine whether cystic fibrosis (CF) patients in Belgium shared Pseudomonas aeruginosa genotypes and to compare the genotypes of isolates from the same patients during two consecutive years. A Belgian databank of the P. aeruginosa genotypes of all colonised CF patients was created.Sputum samples from a total of 276 P. aeruginosa colonised patients during 2003, and from a subgroup of 95 patients in 2004, were analysed. Patients were asked about any social contact between each other by questionnaire. All P. aeruginosa isolates exhibiting different colonial morphology on McConkey agar were first genotyped using arbitrarily primed PCR, whereafter single representatives of each randomly amplified polymorphic DNA-type were further genotyped by fluorescent amplified fragment length polymorphism analysis.In the 213 patients from whom P. aeruginosa could be cultured (resulting in 910 isolates), a total of 163 genotypes were found. The majority (75%) of patients harboured only one genotype. In most of the limited number of clusters, previous contacts between patients could be suspected. In 80% of the patients studied during both years, P. aeruginosa genotype remained unchanged.In conclusion, most colonised cystic fibrosis patients harbour only one Pseudomonas aeruginosa genotype, despite showing different colonial morphotypes. The number of clusters is limited, and most patients seem to retain the same genotypic strain during both years.

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“…Immune complexes are likely to occur in C F patients who have high specific antibody titers for PA (11. 20) together with heavy airway infection with these bacteria (5,14). Several reports confirm their presence in CF serum (18,19,25).…”

Section: Discussionmentioning

confidence: 60%

Influence of Cystic Fibrosis Plasma on Lymphocyte Responses to Pseudomonas Aeruginosa In Vitro

et al. 1981

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Bacterial Flora of Respiratory Tract in Patients with Cystic Fibrosis, 1950-1971

Cited by 88 publications

References 7 publications

Characterization by phenotypic and genotypic methods of metallo-β-lactamase-producing Pseudomonas aeruginosa isolated from patients with cystic fibrosis

Characterization by phenotypic and genotypic methods of metallo-β-lactamase-producing Pseudomonas aeruginosa isolated from patients with cystic fibrosis

Survey ofPseudomonas aeruginosagenotypes in colonised cystic fibrosis patients

Influence of Cystic Fibrosis Plasma on Lymphocyte Responses to Pseudomonas Aeruginosa In Vitro

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