2005
DOI: 10.1097/01.cji.0000154246.89630.6f
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Bacterial Ghosts as Novel Efficient Targeting Vehicles for DNA Delivery to the Human Monocyte-Derived Dendritic Cells

Abstract: Recombinant bacterial ghosts loaded with plasmids were tested as an antigen delivery system and as a potential mediator of maturation for human monocyte-derived dendritic cells (DCs). Bacterial ghosts are cell envelopes derived from Gram-negative bacteria; the intracellular content is released by the controlled expression of plasmid-encoded lysis gene E of PhiX174. All the cell surface structures of the native bacteria, including the outer membrane proteins, adhesins, LPS, lipid A, and peptidoglycans, are pres… Show more

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Cited by 51 publications
(29 citation statements)
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“…Bacterial ghosts have been tested in different animal models for their ability to induce mucosal immune responses (for reviews, see references 24, 39, and 51). In cell culture experiments, bacterial ghosts were taken up very efficiently by dendritic cells and macrophages expressing plasmid-borne marker genes which were delivered together with the bacterial ghosts (27,38).…”
Section: Discussionmentioning
confidence: 99%
“…Bacterial ghosts have been tested in different animal models for their ability to induce mucosal immune responses (for reviews, see references 24, 39, and 51). In cell culture experiments, bacterial ghosts were taken up very efficiently by dendritic cells and macrophages expressing plasmid-borne marker genes which were delivered together with the bacterial ghosts (27,38).…”
Section: Discussionmentioning
confidence: 99%
“…72 The inner space of BGs empty envelope can be loaded with a combination of peptides, drugs or foreign DNA which gives us an opportunity to design new types of polyvalent vaccines. 57,71,73,74 We have shown that BGs loaded with plasmid DNA encoding green fluorescent protein (GFP) are efficiently internalized and phagocytized by both professional antigen presenting cells (APCs) and tumor cells. BGs were able to deliver the heterologous genes to both bacteria before lysis and become elements of the BGs (Fig.…”
mentioning
confidence: 99%
“…Importantly, no cytotoxic impact was observed on target cells. 32,71,74,75 Intradermal and intramuscular immunization of Balb/c mice with BGs loaded with pCMV encoding beta-galactosidase stimulated more efficient humoral and cellular AG-specific immune responses than naked DNA. Beta-galactosidase-specific immune response was detected after intravenous immunization of mice with autologous dendritic cells (DCs) transfected ex vivo with pCMVbeta-loaded BGs.…”
mentioning
confidence: 99%
“…31,32 It should be mentioned that in contrast to other DNA transfer systems the BG-DNA transfer agent is not harmful to the recipient cells. 76 Therefore, it is not astonishing that good immune responses were observed when compared to naked DNA in mice DNA vaccination experiments. It was also found that the Th2 (humoral) immune response against the DNA encoded antigen was stronger than the Th1 (cellular) response, which is rather unusual for DNA vaccines, and can most likely be attributed to the delivery and BG induced signaling.…”
Section: © 2 0 0 8 L a N D E S B I O S C I E N C E D O N O T D I S mentioning
confidence: 99%