Bacterial ghosts – biological particles as delivery systems for antigens, nucleic acids and drugs

Microencapsulation for the improved delivery of bioactive compounds into foods

“…Once the bioactive components responsible for the health beneficial effects of probiotics are known, it might be found that cell viability is not in fact a pre-requisite for the health-promoting health effect [26 ]. In such instances, bacterial 'ghosts' could serve as the ME delivery vehicle for the probioticderived bioactive components [27]. This would be particularly appropriate for intracellular ingredients, such as enzymes, which would be inactivated by the acid of the stomach.…”

Bacterial Ghosts as antigen and drug delivery system for ocular surface diseases: Effective internalization of Bacterial Ghosts by human conjunctival epithelial cells

“…Bacterial Ghosts (BGs) represent empty bacterial cell envelopes of Gram-negative bacteria developed as a novel carrier for DNA, Ags, enzymes, drugs, and adjuvant system for the delivery of mucosal (Eko et al, 2008;Kudela et al, 2010;Tabrizi et al, 2004). BGs are produced by protein E-mediated lysis of Gram-negative bacteria.…”

Minicircle DNA Immobilized in Bacterial Ghosts: In vivo Production of Safe Non-Viral DNA Delivery Vehicles

“…The resulting bacterial ghosts retain the structural integrity of native cell envelopes. Therefore, they represent excellent vaccine candidates as well as protein and/or DNA delivery vehicles with intrinsic adjuvant properties [Eko et al, 2003;Ebensen et al, 2004;Tabrizi et al, 2004;Walcher et al, 2004;Paukner et al, 2005]. Bacterial ghost-mediated DNA delivery showed high effi ciency in the transfection of macrophages and primary dendritic cells (52-60%) and resulted in the elicitation of a more effi cient humoral and cellular immune response compared to naked DNA [Ebensen et al, 2004;Paukner et al, 2005].…”