Bacterial glycobiotechnology: A biosynthetic route for the production of biopharmaceutical glycans

Paliya, Balwant Singh; Sharma, Vivek; Tuohy, Maria G.; Singh, Harikesh Bahadur; Koffas, Mattheos; Benhida, Rachid; Tiwari, Brijesh K.; Kalaskar, Deepak M.; Singh, Brahma N.; Gupta, Vijai Kumar

doi:10.1016/j.biotechadv.2023.108180

Cited by 4 publications

(1 citation statement)

References 218 publications

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“…In addition to the multitude of enzymes that exist in nature, in recent years, different mutant enzymes have been developed that have had their hydrolytic capacity eliminated, greatly improving their synthesis yields [8]. Finally, in some cases, there are described processes in which different cell lines have been used to catalyze reactions involving glycosidase enzymes [9].…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Glycosylation Strategies in the Production of Bioactive Compounds

Andreu,

Ćorović,

Garcia-Sanz

et al. 2023

Catalysts

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Enzymatic glycosylation is a versatile and sustainable biotechnological approach that plays a pivotal role in the production of bioactive compounds. This process involves the enzymatic transfer of sugar moieties onto various acceptor molecules, such as small molecules, peptides, or proteins, resulting in the synthesis of glycosides. These glycosides often exhibit enhanced bioactivity, improved solubility, and enhanced stability, making them valuable in pharmaceuticals, nutraceuticals, and the food industry. This review explores the diverse enzymatic glycosylation strategies employed in the synthesis of bioactive compounds. It highlights the enzymatic catalysts involved, including glycosyltransferases, glycosidases, glycophosphorylases, and glycosynthases. It considers the advantages and disadvantages of these biocatalysts in the stereoselective and regioselective synthesis of different types of glycosylated molecules, phenolic and aliphatic alcohols, oligosaccharides, polysaccharides, glycoderivatives, glycopeptides, and glycoproteins with a clear focus on food and pharmaceutical chemistry. Furthermore, the review outlines various sources of sugar donors, activated glycosides, and sugar nucleotides, as well as the utilization of engineered enzymes and microorganisms for glycosylation reactions. The advantages of enzymatic glycosylation, including its high regioselectivity, stereoselectivity, and sustainability, are emphasized. Therefore, these approaches combining the use of different catalytic systems, the improvement of tools such as immobilization technology or chemical or genetic modification to improve the glycosylation process, could be useful tools in continuous biotechnological advancements.

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Section: Introductionmentioning

confidence: 99%