Bacterial hazard identification and exposure assessment of raw milk consumption in Jimma zone, South West Ethiopia

Gume, Beje; Berhanu, Leykun; Kassa, Tesfaye; Bediru, Habib; Fikre, Assegid Getaneh; Dadi, Lelisa Sena; Mereta, Seid Tiku

doi:10.1186/s12866-023-02910-0

Cited by 7 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The microbial agents mainly coliform, i.e., E. coli, S. aureus, Salmonella spp., Campylobacter spp. and Listeria monocytogenes are microbes that potentially contaminate foods, and pose public health threat (Osafo et al 2022;Rafiq et al 2022;Gume et al 2023). The present study was undertaken based on the law of the Republic of Indonesia # 7 of 1996 concerning food that food of animal products must meet the requirements of safe, healthy, wholesome, and halal (ASUH) for human consumption and the inner peace of the society.…”

Section: Discussionmentioning

confidence: 99%

Microbial Investigation of Animal Product Hygiene in Bali and Nusa Tenggara of Indonesia

2023

Int J Vet Sci

View full text Add to dashboard Cite

Products of animal origin, namely meat, eggs, and milk, must have good value and quality for human benefit and must be monitored regularly. The present study aimed to review the epidemiological mapping of the level of microbial contamination based on surveillance data in the Provinces of Bali and Nusa Tenggara. During 2017, a total of 1875 samples were collected from targeted places that did not have a veterinary control number (VCN) such as slaughterhouses, traditional markets, retail shops and meat supply companies (importers), then brought to the Disease Investigation Center (DIC) Denpasar for analysis. Microbial contamination was tested using the total plate count (TPC) method, according to laboratory procedures for DIC Denpasar. The test results showed that 56.9% of the samples contained germs that exceeded the maximum microbial contamination limit (MMCL) stipulated in SNI 7388: 2009, namely 1x10 6 colonies/g. The contaminant germs were dominated by E. coli (73.8%). There was a highly significant (P<0.001) difference in the number of E. coli that exceeded the maximum limit microbial contamination (MLMC) values detected between the three provinces, the four types of samples tested, and the type of slaughterhouses/markets. However, all samples tested did not contain pathogenic bacteria such as Staphylococcus aureus, Salmonella spp., and Campylobacter spp. This data suggested that the hygiene level of animal products in the region was low which can threaten the health of consumers.

show abstract

Section: Discussionmentioning

confidence: 99%

Microbial Investigation of Animal Product Hygiene in Bali and Nusa Tenggara of Indonesia

2023

Int J Vet Sci

View full text Add to dashboard Cite

show abstract

“…This led to studies on milk and milk products to detect LMO to prevent and control its transmission. In this context, LMO has been detected from locally-fermented fresh milk in Nigeria ( Kawo and Bello, 2016 ), traditionally fermented milk in Uganda ( Mugampoza et al, 2011 ), raw milk (from cattle herds) in Nigeria ( Yakubu et al, 2012 ), Botswana ( Morobe, 2009 ), Ethiopia ( Gebretsadik et al, 2011 ; Gume et al, 2023 ), Tanzania ( Msalya, 2017 ), Uganda ( Mugampoza et al, 2011 ) and Algeria ( Hamdi et al, 2007 ), camel milk in Egypt ( Yehia et al, 2016 ), yoghurt in Nigeria ( Kawo and Bello, 2016 ), dairy products in Uganda ( Mugampoza et al, 2011 ) and Algeria ( Hamdi et al, 2007 ), and cheese (cottage and soft) in Botswana ( Morobe, 2009 ), Ethiopia ( Gebretsadik et al, 2011 ) and Nigeria ( Adetunji et al, 2014 ; Table 2 , Figure 3 ). A recently published review on the subject reported a global prevalence of 4.3% in dairy products in Africa with regional variations.…”

Section: Characteristics and Distribution Of Listeriamentioning

confidence: 99%

A review of the literature of Listeria monocytogenes in Africa highlights breast milk as an overlooked human source

Sarr,

Alou,

Padane

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

According to the latest WHO estimates (2015) of the global burden of foodborne diseases, Listeria monocytogenes is responsible for one of the most serious foodborne infections and commonly results in severe clinical outcomes. The 2013 French MONALISA prospective cohort identified that women born in Africa has a 3-fold increase in the risk of maternal neonatal listeriosis. One of the largest L. monocytogenes outbreaks occurred in South Africa in 2017–2018 with over 1,000 cases. Moreover, recent findings identified L. monocytogenes in human breast milk in Mali and Senegal with its relative abundance positively correlated with severe acute malnutrition. These observations suggest that the carriage of L. monocytogenes in Africa should be further explored, starting with the existing literature. For that purpose, we searched the peer-reviewed and grey literature published dating back to 1926 to date using six databases. Ultimately, 225 articles were included in this review. We highlighted that L. monocytogenes is detected in various sample types including environmental samples, food samples as well as animal and human samples. These studies were mostly conducted in five east African countries, four west African countries, four north African countries, and two Southern African countries. Moreover, only ≈ 0.2% of the Listeria monocytogenes genomes available on NCBI were obtained from African samples, contracted with its detection. The pangenome resulting from the African Listeria monocytogenes samples revealed three clusters including two from South-African strains as well as one consisting of the strains isolated from breast milk in Mali and Senegal and, a vaginal post-miscarriage sample. This suggests there was a clonal complex circulating in Mali and Senegal. As this clone has not been associated to infections, further studies should be conducted to confirm its circulation in the region and explore its association with foodborne infections. Moreover, it is apparent that more resources should be allocated to the detection of L. monocytogenes as only 15/54 countries have reported its detection in the literature. It seems paramount to map the presence and carriage of L. monocytogenes in all African countries to prevent listeriosis outbreaks and the related miscarriages and confirm its association with severe acute malnutrition.

show abstract

Assessing the Microbial Contamination Levels in Milk Samples from Rural and Urban Areas: A Focus on Raisen and Bhopal Districts

Mukhopadhyay,

Malviya,

Barik

et al. 2024

Macromolecular Symposia

View full text Add to dashboard Cite

This study aimed to assess the level of microbial contamination in raw cow milk samples collected from farms in rural and urban regions of Raisen and Bhopal districts, Madhya Pradesh, India. Recent data are gathered to investigate the microbial quality of the milk samples directly, rather than relying on reported findings from other sources. The results reveal that both rural and urban milk samples exhibit significantly higher bacterial colony counts compared to acceptable limits for total viable count. The average total viable count for urban samples is 8.699 log CFU mL−1, with a standard deviation of 0.821 log CFU mL−1, using a sample size of 1 mL. Moreover, urban samples show a higher initial incubation count (8.728 ± 0.431 log CFU mL‐1), indicating potential unsanitary milk handling practices in urban areas. In contrast, rural milk samples demonstrate significantly lower bacterial counts (8.0778 ± 0.079 log CFU mL−1) and exhibit superior laboratory sanitization measures. Enteric bacteria such as Escherichia coli, Enterobacter species, Klebsiella species, and Shigella are found in both urban and rural milk samples after isolation. However, strains of bacterial culture specific to Pseudomonas and Salmonella species are exclusively detected in urban samples, suggesting a higher contamination level in urban areas compared to rural regions. These findings highlight the urgent need for improved milk production and handling practices in both rural and urban areas, particularly in urban settings where the contamination levels are found to be significantly higher. The results of this study contribute to the understanding of microbial quality in raw cow milk and emphasize the importance of implementing appropriate interventions to ensure the safety and quality of milk consumed by the population.

show abstract

Bacterial hazard identification and exposure assessment of raw milk consumption in Jimma zone, South West Ethiopia

Cited by 7 publications

References 23 publications

Microbial Investigation of Animal Product Hygiene in Bali and Nusa Tenggara of Indonesia

Microbial Investigation of Animal Product Hygiene in Bali and Nusa Tenggara of Indonesia

A review of the literature of Listeria monocytogenes in Africa highlights breast milk as an overlooked human source

Assessing the Microbial Contamination Levels in Milk Samples from Rural and Urban Areas: A Focus on Raisen and Bhopal Districts

Contact Info

Product

Resources

About