Bacterial heat shock proteins directly induce cytokine mRNA and interleukin-1 secretion in macrophage cultures

Retzlaff, Cassandra L; Yamamoto, Yoshimasa; Hoffman, Paul S.; Friedman, Herman; Klein, Thomas

doi:10.1128/iai.62.12.5689-5693.1994

Cited by 151 publications

(79 citation statements)

References 29 publications

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“…The chaperon function of Hsp is of major importance for protein synthesis; the ubiquitous distribution of Hsp and their high sequence conservation pose interesting challenges to the immune system. During macrophage activation, in which a wide variety of polypeptides are induced, increased levels of Hsp (both mRNA and proteins) are produced [34]. The diet composition may, by altering lymphocyte activation, have a high in£uence on autoimmune processes.…”

Section: Discussionmentioning

confidence: 99%

The effect of dietary lipid manipulation on murine splenic lymphocytes apoptosis and heat shock protein over expression

Carratelli

1999

FEMS Immunology and Medical Microbiology

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In this study, we kept BALB/c mice on a hyperlipidic diet for 120 days and then assessed the predisposition to apoptosis and the appearance of heat shock protein (Hsp) on splenic lymphocytes. By immunoblot analysis, bands corresponding to Hsp 60 and Hsp 70 in cells from mice kept on a saturated fatty acid diet showed a greater expression already after 1 month while two other bands, which correspond to Hsp 25 and Hsp 27, were slightly present after 1 month of treatment. In cells from mice kept on a diet rich in unsaturated fatty acid, there was a marked expression of Hsp 25 and Hsp 27 after only 30 days of treatment, which was maintained constant for up to 4 months; while for bands corresponding to Hsp 60 and Hsp 70, a significant minor signal was only detectable after 2-4 months from the beginning of the treatment. Splenic lymphocytes from animals kept on a lipidic diet containing saturated fatty acids were more susceptible to death by apoptosis, while cells of animals treated with unsaturated fatty acid were shown to be more resistant.

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Section: Discussionmentioning

confidence: 99%

The effect of dietary lipid manipulation on murine splenic lymphocytes apoptosis and heat shock protein over expression

Carratelli

1999

FEMS Immunology and Medical Microbiology

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“…It has already been shown that this low LPS concentration does not induce IL-1b mRNA expression or enhance the expression when added to Lp-hsp 60. 27 In order to further minimize the possible influence of any remaining endotoxin, the Lp-hsp 60 was always diluted in RPMI-1640 medium containing 16 m g/ml polymyxin B. This treatment has been shown to completely neutralize 20 ng of LPS per ml in terms of IL-1b mRNA inducing activity under the experimental conditions chosen.…”

Section: Evaluation Of Lp-hsp 60 Preparation For the Presence Of Lpsmentioning

confidence: 99%

“…It has been shown that various bacterial hsp directly induce increased cytokine mRNA accumulation in macrophages. 27 Thioglycollate-elicited macrophages from BALB/c mice were stimulated with Lp-hsp 60 (5 m g/ml) for 3 hr. The RNA was extracted, reverse transcribed and amplified by PCR as described above.…”

Section: Cellular Il-1b Mrna Steady-state Levels After Stimulation Wimentioning

confidence: 99%

“…This indicates that the inducing activity for IL-1b mRNA is heat labile and is not due to LPS, since LPS has been shown to be fully active in terms of IL-1b mRNA induction after the same preheating procedure. 27 We also examined the effect of Lp-hsp 60, coated, latex beads in the presence of CyD. The cytochalasins have been used extensively to inhibit phagocytosis of particles; however, they have only minimal effects on attachment of the particles to the cell membrane.…”

Section: Cellular Il-1b Mrna Steady-state Levels After Stimulation Wimentioning

confidence: 99%

“…25,26 Consistent with these results we have demonstrated that treatment of murine macrophage cultures with different purified bacterial hsp, including L. pneumophila hsp 60 (Lp-hsp 60), increased the steady-state levels of cytokine mRNA for IL-1a, IL-1b, IL-6, TNF-a, and granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as IL-1 secretion into the culture supernatant, suggesting that bacterial hsp directly modulate macrophage immunity. 27 This effect was shown not to be due to contamination of the hsp preparations with bacterial lipopolysaccharides (LPS). However, the molecular mechanisms by which the hsp acted were not identified.…”

Section: Introductionmentioning

confidence: 99%

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Legionella pneumophila heat‐shock protein‐induced increase of interleukin‐1β mRNA involves protein kinase C signalling in macrophages

et al. 1996

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SUMMARYHeat-shock proteins (hsp) are chaperon molecules important in protein folding and assembly. Furthermore, they may have functions in immunoregulatory processes, like T-cell stimulation and antigen presentation, which are not yet fully understood. It has been shown that several hsp of various species and family derivations modulate functions in macrophage immunity by directly increasing cytokine production. In the present study we showed that the 60 000 MW hsp of Legionella pneumophila (Lp-hsp 60) increased cellular steady-state levels of interleukin-1b (IL-1b) mRNA measured by quantitative reverse transcription-polymerase chain reaction and Northern blotting as well as IL-1 secretion, when added to cultures of thioglycollate-elicited mouse peritoneal macrophages in vitro. The level of mRNA increased in a dose-dependent manner with a minimum effective concentration of 0 . 5 m g/ml and peaked 3 hr after stimulation. Lp-hsp 60-coated latex beads also increased IL-1b mRNA levels in the presence of cytochalasin D, which inhibits bead uptake but permits binding, indicating that binding to the macrophage surface was sufficient for induction. Accumulation of IL-1b mRNA was completely blocked by pretreatment with the protein kinase C (PKC) inhibitor, H7, but not decreased by prior treatment with cycloheximide. The cell lysates of macrophages stimulated with Lp-hsp 60 showed an increased PKC activity measured by phosphorylation of PKC pseudosubstrate. The IL-1 bioactivity in culture supernatants after 24 hr of stimulation with Lp-hsp 60 was increased in a dose-dependent manner but at hsp concentrations in excess of those needed to increase mRNA. Thus, the present study demonstrates that Lp-hsp 60 rapidly increases the steady-state level of IL-1b mRNA, possibly through a cell surface receptor system involving a PKC-dependent signalling pathway.

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The Mycobacterium tuberculosis chaperonin 10 monomer exhibits structural plasticity

et al. 2004

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The conditions which favor dissociation of oligomeric Mycobacterium tuberculosis chaperonin 10 and the solution structure of the monomer were studied by analytical ultracentrifugation, size exclusion chromatography, fluorescence, and circular dichroism spectroscopies. At neutral pH and in the absence of divalent cations, the protein is fully monomeric below approximately a 4.7 microM concentration. Under these conditions the monomer forms completely unfolded and partially folded conformers which are in equilibrium with each other. One conformer accumulates over the others which is stable within a very narrow range of temperatures. It contains a beta-sheet-structured C-terminal half and a mostly disordered N-terminal half. Other components of the equilibrium include partially helical structures which do not completely unfold at high temperature or under strong acidic conditions. Complete unfolding of the monomer occurs in the presence of denaturants or below 14 degrees C. Cold-denaturation is detected at an unusually high temperature and this may be due to the concentration of hydrophobic residues, which is larger in chaperonins than in other globular proteins. Finally, the monomer self-associates in the pH range 5.8-2.9, where it forms small oligomers. A structure-activity relationship was investigated with the sequences known to be involved in the various biological activities of the monomer.

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Bacterial heat shock proteins directly induce cytokine mRNA and interleukin-1 secretion in macrophage cultures

Cited by 151 publications

References 29 publications

The effect of dietary lipid manipulation on murine splenic lymphocytes apoptosis and heat shock protein over expression

The effect of dietary lipid manipulation on murine splenic lymphocytes apoptosis and heat shock protein over expression

Legionella pneumophila heat‐shock protein‐induced increase of interleukin‐1β mRNA involves protein kinase C signalling in macrophages

The Mycobacterium tuberculosis chaperonin 10 monomer exhibits structural plasticity

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