Bacterial hemolysins as virulence factors

Goebel, Werner; Chakraborty, Trinad; Kreft, Jürgen

doi:10.1007/bf00461864

Cited by 54 publications

(42 citation statements)

References 33 publications

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“…However, recent studies have been performed to identify the gene(s) responsible for hemolytic activity [23] [24]. Even though the hemolytic activity of some Bacillus spp., such as Bacillus subtilis, is less than in pathogens [25], these isolates may be considered unsafe for food or personal health care applications until the effect of this virulence factor is either eliminated, modified, or confirmed as causing no harm to the eukaryotic host.…”

Section: Introductionmentioning

confidence: 99%

Safety Properties and Probiotic Potential of Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895

Algburi¹,

Volski²,

Cugini³

et al. 2016

AiM

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This study reports on the safety and putative probiotic properties of Bacillus amyloliquefaciens B-1895 and Bacillus subtilis KATMIRA1933. According to the bacterial reverse mutation (Ames) test, cell-free supernatants of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 were not mutagenic. The two strains co-aggregated with Escherichia coli and Pseudomonas aeruginosa, and cell-free supernatants inhibited the growth of Streptococcus intermedius and Porphyromonas gingivalis. Endospores of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 were tolerant to 0.3% (w/v) bile salts and survived incubation for 4 h in MRS broth at pH 2.0 to 3.0. The ability of the two strains to produce antimicrobial compounds potentiates their application in health care formulations, personal care products, food and animal feed.

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Section: Introductionmentioning

confidence: 99%

Safety Properties and Probiotic Potential of Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895

Algburi¹,

Volski²,

Cugini³

et al. 2016

AiM

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“…Hemolysins are virulence factors implicated in various kinds of bacterial infection (3,7,28). Acquisition of iron for bacterial metabolism may be facilitated by lysis of host erythrocytes through hemolysin, which releases intracellular iron (20).…”

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confidence: 99%

Characterization of Actinobacillus actinomycetemcomitans Hemolysin

Kimizuka

Miura

Okuda

1996

Microbiology and Immunology

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Twenty out of 33 Actinobacillus actinomycetemcomitans strains formed hemolytic colonies on horse blood agar plates under anaerobic conditions. The hemolytic activity found in A . actinomycetemcomitans strain 137HE was examined. This activity was detected in the late exponential to early stationary phases of growth. Human erythrocytes were the most susceptible, followed by rabbit, sheep, horse and swine red blood cells. The majority of activity was detected in the cell-associated vesicle fraction. Zwitterionic detergent 3-[(3-cholamidopropy1)-dimethyl-ammonio]-1-propanesulfonate (CHAPS) extract from whole cells was semipurified by ammonium sulfate precipitation, preparative isoelectric focusing (IEF) and gel-filtration chromatography to yield a major band on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with a molecular mass of 12 kDa. Heating at 80 C for 30 min and treatment with proteinase K or trypsin resulted in complete disappearance of the hemolytic activity. Sulphydryl reagents enhanced activity and small amounts of cholesterol inhibited it. In summary, we demonstrated the presence of hemolysin in A. actinomycetemcomitans, and examined and characterized it.

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“…17 Therefore, findings of this study are very significant in clinical perspectives, since stress conditions may induce virulent properties in bacterial strains. There is evidence that stresses like starvation, acidic pH and heat shock may induce the expression of some virulence genes.…”

Section: Discussionmentioning

confidence: 91%

Environmental Factors Regulate the hlyE Gene Expression in Both S. typhi and E. coli in a Similar Way to Display Haemolytic Activity

Ahsan¹,

Shamma²,

Ahsan³

et al. 2017

Bangladesh Med Res Counc Bull

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Haemolysin (HlyE) is an essential virulence factor of Salmonella, Escherichia coli and other enteric bacteria. Although, a substantial degree of haemolytic activity is not seen under normal culture conditions in these organisms, however, the non-haemolytic E. coli K-12 showed significant haemolytic activity under stress conditions. To confirm this phenomenon in other enteric bacteria, in this study, the production of haemolysin in Salmonella enterica serovar Typhi under stress conditions, like oxygen and glucose starvations in vitro was investigated during March-December 2015. For this, S. typhi was cultured under oxygen or glucose starvation condition separately and this organism showed high haemolytic activity. The activity was found to be much higher when both the conditions were applied together. Also, the role of the transcription factor SlyA of S. typhi was investigated on induction of haemolytic activity. When E. coli K-12 was transformed with plasmid containing the gene of SlyA, the recombinant bacteria without any starvation condition, also showed similar haemolytic activity that was exhibited by S. typhi grown under oxygen and glucose starvation conditions. All these findings suggest that both environmental factors like oxygen or glucose starvation and overexpression of the transcription factor SlyA have important role in inducing hlyE gene expression in S. typhi.

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Bacterial hemolysins as virulence factors

Cited by 54 publications

References 33 publications

Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895

Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895

Characterization of Actinobacillus actinomycetemcomitans Hemolysin

Environmental Factors Regulate the hlyE Gene Expression in Both S. typhi and E. coli in a Similar Way to Display Haemolytic Activity

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Bacterial hemolysins as virulence factors

Cited by 54 publications

References 33 publications

Safety Properties and Probiotic Potential of &lt;i&gt;Bacillus subtilis&lt;/i&gt; KATMIRA1933 and &lt;i&gt;Bacillus amyloliquefaciens&lt;/i&gt; B-1895

Safety Properties and Probiotic Potential of &lt;i&gt;Bacillus subtilis&lt;/i&gt; KATMIRA1933 and &lt;i&gt;Bacillus amyloliquefaciens&lt;/i&gt; B-1895

Characterization of Actinobacillus actinomycetemcomitans Hemolysin

Environmental Factors Regulate the hlyE Gene Expression in Both S. typhi and E. coli in a Similar Way to Display Haemolytic Activity

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Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895

Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895