Bacterial Identification Based on Universal Gene Amplification and Sequencing

Lau, Susanna K. P.; Teng, Jade L. L.; Woo, Patrick C. Y.

doi:10.1007/978-3-319-95111-9_1

Cited by 1 publication

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“…Currently, PCR, as well as other NAAT and sequencing techniques are part of the standard repertoire of microbiological diagnostics. These procedures are widely used for the direct detection of pathogens or for the molecular identification of cultured microorganisms, for the recognition of resistance genes or virulence factors, and for genotype determination [2]. With regard to the quality and speed of pathogen identification, the introduction of mass spectrometry techniques into routine microbiological diagnostics work-up has been revolutionary.…”

Section: Introductionmentioning

confidence: 99%

Advanced methods of bacteriological identification in a clinical microbiology laboratory

Żukowska¹

2021

J Pre Clin Clin Res.

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Introduction and objective. Conventional, culture-based methods of bacterial identification and drug-susceptibility testing are considered the gold standard in medical microbiology. In recent years, classical microbiological methods have been supplemented with modern analytical and molecular methods. The aim of the review was to discusses the methods which have been permanently adapted to bacteriological microbiological diagnostics. Abbreviated description of the state of knowledge. Currently, PCR, as well as other nucleic acid amplification tests and sequencing techniques, are part of the standard repertoire of microbiological diagnostics. With regard to the quality and speed of pathogen identification, the introduction of mass spectrometry techniques into routine microbiological diagnostics work-up has been revolutionary. Within a short time in many laboratories, Matrix-Assisted Laser Desorption/Ionisation -Time of Flight Mass Spectrometry (MALDI TOF MS) systems have almost completely replaced conventional biochemical pathogen identification. Conclusions. Microbiological diagnostics is an indispensable element of a targeted therapy. The techniques used in the laboratory depend primarily on the laboratory's apparatus, the costs of the analysis, as well as the sensitivity and specificity of a method. However, regardless of the culture-based methods universality, advanced techniques have permanently established themselves in diagnostics. Confident information about the detected organism and treatment possibilities in a combination with the clinical context are conducive to successful therapy. Although modern methods still require validation and close collaboration between clinicians, microbiologists and bioinformaticians, these methods, once deemed to be the future, have already arrived.

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Section: Introductionmentioning

confidence: 99%

Advanced methods of bacteriological identification in a clinical microbiology laboratory

Żukowska¹

2021

J Pre Clin Clin Res.

View full text Add to dashboard Cite

show abstract

Bacterial Identification Based on Universal Gene Amplification and Sequencing

Cited by 1 publication

References 211 publications

Advanced methods of bacteriological identification in a clinical microbiology laboratory

Advanced methods of bacteriological identification in a clinical microbiology laboratory

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