Bacterial Infection in Cystic Fibrosis

May, J. Robert; Herrick, N C; Thompson, Doris H.

doi:10.1136/adc.47.256.908

Cited by 94 publications

(37 citation statements)

References 11 publications

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“…The present study also identified higher resistance to other antibiotics, including tobramycin (51.6%), piperacillin-tazobactam, gatifloxacin, netilmicin (44.2%), ciprofloxacin and gentamicin (35.9%). However, resistance to cefoperazone and cefepime was detected in 28.9% of samples, which is similar to other studies demonstrating reduced susceptibility to commonly used antibiotics (3,4,11,12).…”

Section: Discussionsupporting

confidence: 75%

“…Cystic fibrosis (CF) is an autosomal recessive genetic disease (6); the opportunistic pathogen Pseudomonas aeruginosa may cause chronic lung disease in CF patients, depending on the genetic adaptation of the pathogen, and is a prevalent pathogen in CF patients with pulmonary infection (1)(2)(3)(4)(5). Worldwide, 80% of CF patients were found to be infected with P. aeruginosa (7,8).…”

Section: Introductionmentioning

confidence: 99%

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Characterization by phenotypic and genotypic methods of metallo-β-lactamase-producing Pseudomonas aeruginosa isolated from patients with cystic fibrosis

Zhang

Wang

et al. 2014

Molecular Medicine Reports

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Abstract.Pseudomonas aeruginosa continues to be a predominant cause of infections with high intrinsic resistance to antibiotics, resulting in treatment failure. P. aeruginosa is the leading cause of respiratory infections among cystic fibrosis (CF) patients. Resistance to carbapenem antibiotics among P. aeruginosa has been reported. Thus, this study was undertaken to characterize the metallo-β-lactamase (MBL) production of P. aeruginosa by phenotypic and genotypic methods. A total of 572 sputum samples were collected from cystic fibrosis patients along with the patient demographic details in a questionnaire. In total, 217 P. aeruginosa isolates were collected and an antibiogram revealed that 159 (73.3%) and 141 (64.9%) of these colonies exhibited resistance to imipenem and meropenem, respectively. Ceftazidime and tobramycin resistance were both identified in 112 (51.6%) isolates, and resistance to piperacillin-tazobactam, gatifloxacin and netilmicin was detected in 96 (44.2%) respective samples. A total of 62 (28.6%) respective samples were resistant to cefoperazone, cefepime and ceftriaxone. The least antibiotic resistance was shown to amikacin and ceftizoxime with 51 (23.5%) and 32 (14.7%) respective colonies resistant to the antibiotics. The minimum inhibitory concentration (MIC) for imipenem revealed a reduction in the MIC values. MBL screening by the zone enhancement method using ceftazidime plus EDTA discs demonstrated that 63 (56.25%) of the colonies were positive for MBL. A total of 53 (84.1%) samples expressed blaVIM and 48 (76.1%) expressed blaIMP genes, as detected by duplex polymerase chain reaction. In conclusion, carbapenem resistance is of great clinical concern in cystic fibrosis patients with P. aeruginosa infection. Therefore, mandatory regular screening and monitoring the resistance in P. aeruginosa among CF patients is required.

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Section: Discussionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Characterization by phenotypic and genotypic methods of metallo-β-lactamase-producing Pseudomonas aeruginosa isolated from patients with cystic fibrosis

Zhang

Wang

et al. 2014

Molecular Medicine Reports

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“…We believe that this factor is a ~seudornon&-~~cific bactericidal blocking antibody. It should be noted that all of the CF sputum samples examined in these studies were obtained from patients chronically colonized/ infected with P. aeruginosa and hence expected to contain antiPseudomonas immunoglobulins (13,15). The presence of IgA and IgG antibodies against surface antigens of P. aeruginosa in sputum has been previously described (17).…”

Section: From Thismentioning

confidence: 99%

“…Despite the presence of elevated levels of Pseudomonas antibodies in CF patients' sera and pulmonary secretions (13,15), these bacteria are not cleared by pulmonary defense mechanisms. Alveolar macrophages isolated from CF patients appear normal (B), but CF senun appears to interfere with the ability of rabbit and human alveolar macrophes to ingest and destroy Pseudomonas (2, 3,5,21,23).…”

mentioning

confidence: 99%

Pseudomonas-Infected Cystic Fibrosis Patient Sputum Inhibits the Bactericidal Activity of Normal Human Serum

Schiller

Millard

1983

Pediatr Res

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CYSTIC FIBROSIS 747 nebulizer). However, the adsorption rate in Figure 2C is still more rapid than that of vorkxed CLL dispersions in Figure 1 for the uniform fmal dispersion concentration (5 mg CLL/71 ml subphase) used (see "Materials and Methods"). This dilute concentration was chosen because it allow a disaimination between the adsorption isotherms of CLL (Si) and CLL (V). At h@m concentdona (z 0.25 mg/ml) vortexed CLL dqcrsions adsorb as rapidly as ch u m i d dispersions in Figure 2 SummpryChronic obstructive vulmonarv infection is the maior cause of Sputum samples were obtpined from nkteen cystic fibrosis (CF) patients with respiratory tract infectiom due to Reuhnonm rrmrginasa and were exrmined for the presence of a specific local protective or blacking fador, which might partrally explain the inability of CF patients' pulmonary defense mechanisms to clear their lungs of Reudomonm infection. Using an assay that measures the bactericidal activity of fresh nonnd human sernm (FHS), eight-of nineteen CF sputa examined were capable of protecting either autdogous or heterdogous strains of P. uenrgtmccr from serum bactericidal activity. Most of this protective activity was absorbable at 4OC by P s e r r d o m , and could be reduced by dilution In phosphate buffered sdim or counteracted by an i~-crease in senun concentration. This protective activity is believed to be due to a Reudomomq-specific bactericidal blocking antibody. In contrast, only two of 17 non-CF sputa were found to significantly inbibit the bactericidal activity of FHS for P. aeruginosa Furthermore, three out of five CF sputa also protected Escherichiu eoh' from the bactericidal activity of FHS. This protective activity was mucb less tbrn that observed with P. aerughosu, and was not absorbable at 4"C, suggesting the presence of a second blocking or protective factor in CF sputum. From these observatioos, we conclude that sputum from CF patients infected with P. aerughoaa contains one or more factors that interfere with the bactericidal activity present in FHS.Abbreviations BHI, brain heart infusion CF, cystic fibrosis CFU, colony fonning unit FHS, fresh normal human serum PBS, plumphate buffered saline PIA, pseudomonas isolation agar sIgA, secretory IgA morbidity and mortaliiy in people with cystic fibroiis (CF). Although respiratory infection with Staphylococcur aureus often occurs, ~se;domo&s aetuginosa ( p~i & r l y the mucoid colonial form) eventually emerges as the dominant pathogen in 70-905b of CF patients, and > 90% die of progressive pulmonary insufftciency complicated by infection with P. aenrginosa (4).Except for respiratory tract infections, CF children are not more susceptible to infection than normal children of comparable age. P. aenrginosa is known to be an opportunistic pathogen, which commonly infects the debilitated patient (25,26), and is seldom isolated from pediatric patients with chronic respiratory disease other than CF (4, 11). These basic clinical observations, coupled with the fact that pulmonary infections rarely spread...

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“…Non-typable (NT) Haemophilus incfluenzae strains are frequently cultured from purulent lower respiratory tract secretions of patients suffering from chronic bronchitis, bronchiectasis and cystic fibrosis (May et al, 1972;Smith et al, 1976;Murphy and Apicella, 1987). Several authors have examined the outer membrane protein (OMP) profile of NT H. incfluenzae (see Murphy and Apicella, 1987).…”

Section: Introductionmentioning

confidence: 99%

Porphyrin ring source can alter the outer membrane protein profile of non-typable Haemophilus influenzae

Maciver

O'Reilly²,

Brown³

1990

Journal of Medical Microbiology

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Summary.Porphyrin ring source appears to alter the outer membrane protein (OMP) profile of some, but not all, non-typable (NT) Haemophilus inzuenzae strains isolated from sputum. When haemin was replaced with protoporphyrin IX, 41% of strains examined produced increased amounts of a polypeptide of 84 Kda and new OMPs of either 120 or 150 Kda. Immunoblotting with paired patient's sera revealed that antibodies reactive with these proteins were present, demonstrating OMP antigenicity and expression in vivo and indicating that these isolates of NT H . inzuenzae may display an altered OMP phenotype when growing in the human lung.

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Bacterial Infection in Cystic Fibrosis

Cited by 94 publications

References 11 publications

Characterization by phenotypic and genotypic methods of metallo-β-lactamase-producing Pseudomonas aeruginosa isolated from patients with cystic fibrosis

Characterization by phenotypic and genotypic methods of metallo-β-lactamase-producing Pseudomonas aeruginosa isolated from patients with cystic fibrosis

Pseudomonas-Infected Cystic Fibrosis Patient Sputum Inhibits the Bactericidal Activity of Normal Human Serum

Porphyrin ring source can alter the outer membrane protein profile of non-typable Haemophilus influenzae

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