Bacterial inhibition efficiency of prilocaine and bupivacaine

Abstract

This study aimed to demonstrate the antibacterial effects of bupivacaine and prilocaine on Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. In our study, the in vitro antimicrobial effects of 20 mg/mL prilocaine and 5 mg/mL bupivacaine were tested against a S. aureus American‐type culture collection (ATCC) 29213, P. aeruginosa ATCC 27853, and E. coli ATCC 25922, divided into Group P (Prilocaine) and Group B (Bupivacaine), respectively. S. aureus ATCC 29213, P. aeruginosa ATCC 27853, and E. coli ATCC 25922 were cultured on Mueller Hinton agar (Oxoid, Basingstoke, UK) plates for 18 to 24 hours at 37°C. In terms of inhibition zone diameters, inhibition of S. aureus ATCC 29213 was observed in both groups at the 12th and 24th hours. The 12th‐ and 24th‐hour S. aureus ATCC 29213 value was significantly higher in Group P compared with Group B (P = .008). At the 12th and 24th hours, inhibition of E. coli ATCC 25922 was observed in both groups. The 12th‐ and 24th‐hour E. coli ATCC 25922 value was significantly higher in Group P compared with Group B (P = .008). In our study, it was seen that prilocaine and bupivacaine had an antimicrobial effect on S. aureus and E. coli. In the comparison between these two local anesthetics (LAs), this effect was found to be significantly higher in prilocaine than bupivacaine. Therefore, we are of the opinion that antimicrobial effect potentials should also be taken into account in the selection of an LA agent in order to prevent the complications of an infection that might develop during LA infiltration and might lead to serious morbidity.

Caudal Block versus Wound Infiltration for Inguinal Procedures in Young Children: A Randomized Clinical Study

“…Different agents have been found to have differing antibacterial properties. [22][23][24] We did not see any wound infections in our cohort, and even if we had seen some, the sample size would have been too small to draw a meaningful conclusion. Nevertheless, the proven antibacterial effect of a local wound infiltration, especially in the groin should be considered as an argument in favor of LI.…”

The effect of local anesthetics against planktonic forms and film formation of S. aureus strains and its dependence on antiseptics activity

“…It is known that bacteria in the biofilm pose a much greater threat to human life and health ( Kesici et al, 2019 ). Therefore, the key to successful treatment of infectious postoperative complications is the use of antimicrobial agents that affect not only planktonic forms of bacteria, but also their biofilmforming potential.…”

Antimicrobial effects of fentanyl and bupivacaine: an in vitro study

“… 1 In the literature, many cases were reported where regional (spinal, epidural) anesthesia and analgesia applications related central nervous system infections such as epidural abscess and meningitis developed. 5 , 12 , 13 , 14 , 15 It is known that central nervous system infections can result in severe morbidity that extends to paraplegia. Therefore, it is of utmost importance in the clinical practice to utilize antimicrobial effect potentials of LAs, as well as paying attention to asepsis rules in order to prevent LA application related wound infections and central nervous system infections.…”

“…Regional anesthesia is widely used in modern practice and some studies report an increase in the development of infections related to regional anesthesia and analgesia applications. 4 , 5 , 10 , 13 , 16 Therefore, utilization of the antimicrobial effect potentials of anesthetic agents gains importance, aside from paying attention to asepsis rules during the application. It is observed in this context that the number of studies in the literature regarding the antimicrobial effects of fentanyl + LA combinations is limited.…”