BACTERIAL INHIBITORY EFFECT OF ALGERIAN POMEGRANATE (Punica Granatum L.)  EXTRACTS (PEEL, JUICE, AND SEED) AGAINST MULTIDRUG RESISTANT BACTERIA

DEBIB, Aicha; Menadi, Soumaya; SAHNOUNI‎, Fatima; Boukhatem, Mohamed Nadjib; OUAHCHIA, Celia; KACED, Amel

doi:10.55251/jmbfs.4622

Cited by 4 publications

(5 citation statements)

References 19 publications

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“…Besides, the BP had an effect on B. cereus, S. aureus and P. aeruginosa with MBC values of 50, 100 and 100 mg/mL, respectively. In previous study, Debib et al 69 showed that petroleum ether, ethanolic and acetonic peel extracts inhibited the growth of E. coli with inhibition zones ranging from 14 to 28 mm and MIC values ranging from 8 to 256 μg/mL; in contrast, our fractions did not show any effect against this bacteria. The activity of pomegranate peels against microbes can be attributed to its high polyphenolic content, particularly its flavonoids and tannins.…”

Section: Antibacterial Activitycontrasting

confidence: 52%

“…70 One of the anti-infective properties of tannins is causing membrane instability, 56 and for flavonoids is forming complexes with various proteins found inside the bacterial cell walls or extracellular proteins, 68 consequently, causing leakage of the cell membrane and cell lysis, which ultimately leads to microorganism death. 69 The results above indicate the richness of AP and BP in tannins and flavonoids, in addition to the efficient capacities of extracts as antioxidants, which may explain their antibacterial effect (inhibition zone: 13-25 mm, MIC: 0.39-12.5 mg/mL and MBC: 25-100 mg/mL). The good activity of BP against P. aeruginosa may be interpreted by the higher content of flavonoids and flavonols compared to the ethyl acetate fraction.…”

Section: Antibacterial Activitymentioning

confidence: 81%

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In vitro Antioxidant, anti-Alzheimer and Antibacterial Activities of Ethyl acetate and n-Butanol Fractions of Punica granatum Peel from Algeria

2023

TJNPR

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Polyphenols are secondary plant metabolites and are naturally present in almost all plant materials. 7 Alzheimer's disease is the most common cause of dementia in the elderly population. Clinically, AD is characterized initially by mild memory impairment that declines progressively to the ultimate loss of mental and physical skills. 8 Acetylcholine (ACH) is a signaling molecule in neurotransmission; one of the few therapeutic approaches for AD is cholinesterase inhibitors. By inhibiting the activity of the enzymes acetylcholinesterase and butyrylcholinesterase, which are involved in acetylcholine hydrolysis, ACH levels at synapses can be increased. 9 Plants are estimated to be a potential source of novel cholinesterase inhibitors. 10 Microorganisms are ubiquitous and can cause huge health problems in humans. 11 Staphylococcus aureus is considered one of the most common sources of food borne diseases, while Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa produce toxins and other metabolites that induce human gastroenteritis diseases. 12 The pathogens can build resistance against antibiotics, 13,14 furthermore, synthetic antibiotic residues have been polluting the environment and ecosystem survival. 15 Plants are non toxic or with low toxicity, 16 they are regarded as a valuable source of naturally antimicrobial agents. 17

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Section: Antibacterial Activitycontrasting

confidence: 52%

Section: Antibacterial Activitymentioning

confidence: 81%

In vitro Antioxidant, anti-Alzheimer and Antibacterial Activities of Ethyl acetate and n-Butanol Fractions of Punica granatum Peel from Algeria

2023

TJNPR

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Section: Introductionmentioning

confidence: 99%

“…14,16 Components of pomegranate influence transcription factors, anti-apoptotic proteins, pro-apoptotic proteins, cell cycle regulator molecules, pro-inflammatory mediators, cell adhesion molecules, protein kinases, and growth factors in multiple types of cancer. 19 The ongoing work's aim was to trace the pomegranate seed and peel ethanolic extracts' anticancer activity against liver cancer cell line, namely HepG2 and related histopathological, immunohistochemical, genetic and oxidative stress profile.…”

Section: Introductionmentioning

confidence: 99%

Pomegranate Seeds and Peel Ethanolic Extracts Anticancer Potentials and Related Genetic, Histological, Immunohistochemical, Apoptotic and Oxidative Stress Profiles: In vitro Study

Nasr¹,

Naeem²,

El-Shenbaby³

et al. 2023

JEP

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Introduction Owing to their great quantity of hydrolyzable anthocyanins and tannins, the peel and seeds of pomegranate are edible and possess potent anti-oxidant and anti-inflammatory characteristics. This work aims to trace the pomegranate seed and peel ethanolic extracts’ anticancer activity against liver cancer cell line, namely HepG2 and related histopathological, immunohistochemical, genetic and oxidative stress profile. Methods In vitro study for both seed and peel extract showed the prevalence of phenols, polyphenols and acids, those have anti-proliferative potential against liver cancer cell line (HepG2) with 50% inhibitory concentration (IC50) of seed significantly reduced that of peel. Toxicity of test extracts was concentration dependent and accompanied with cell cycle arrest and cell death at theG0/G1 and S phases but not at the G2/M phase. Cell arrest was supplemented with raised ROS, MDA and decreased SOD, GSH and Catalase. Results and discussion Apoptosis-related genes showed significant up-expression of pro-apoptotic gene ( P53 ), Cy-C , Bax , and casp-3 and down expression of anti-apoptotic gene ( Bcl-2 ). Also, Casp-3 and P53 proteins were substantially expressed under the effect of test extracts. Histopathological study demonstrated that the untreated cells (control group) were regular cells with nuclear pleomorphism and hyperchromatic nuclei, while seed and peel extracts-treated cells showed necrosis, mixed euchromatin and heterochromatin, intra-nuclear eosinophilic structures, burst cell membranes, and the shrunken apoptotic cells with nuclear membranes and irregular cells. Finally, PCNA gene detected by immunohistochemistry was down regulated significantly under the effect of seed extract treatment than in case of cell medication with peel extract.

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“…The interesting fact is that peel extract has the highest amount of bioactive phenolic compounds such as ellagitannins, tannins [28] and anthocyanin [29,30], including ellagic acid as well as punicalagin (2,3-hexahydroxydiphenoylgallagyl-D-glucose) [31]. Recently, pomegranate extracts were evaluated against beta-lactamase-producing drug resistance Gram-positive and Gram-negative bacteria using agar diffusion and minimum inhibitory concentration assays [27,32].…”

Section: Introductionmentioning

confidence: 99%

Punicalagin, a pomegranate polyphenol sensitizes the activity of antibiotics against three MDR pathogens of the Enterobacteriaceae

Kiran,

Tariq,

Iqbal

et al. 2024

BMC Complement Med Ther

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Background Multidrug resistance (MDR) in the family Enterobacteriaceae is a perniciously increasing threat to global health security. The discovery of new antimicrobials having the reversing drug resistance potential may contribute to augment and revive the antibiotic arsenal in hand. This study aimed to explore the anti-Enterobacteriaceae capability of bioactive polyphenols from Punica granatum (P. granatum) and their co-action with antibiotics against clinical isolates of Enterobacteriaceae predominantly prevalent in South Asian countries. Methods The Kandhari P. granatum (Pakistani origin) extracts were tested for anti-Enterobacteriaceae activity by agar well diffusion assay against MDR Salmonella enterica serovar Typhi, serovar Typhimurium and Escherichia coli. Predominant compounds of active extract were determined by mass spectrometry and screened for bioactivity by agar well diffusion and minimum inhibitory concentration (MIC) assay. The active punicalagin was further evaluated at sub-inhibitory concentrations (SICs) for coactivity with nine conventional antimicrobials using a disc diffusion assay followed by time-kill experiments that proceeded with SICs of punicalagin and antimicrobials. Results Among all P. granatum crude extracts, pomegranate peel methanol extract showed the largest inhibition zones of 25, 22 and 19 mm, and the MICs as 3.9, 7.8 and 7.8 mg/mL for S. typhi, S. typhimurium and E. coli, respectively. Punicalagin and ellagic acid were determined as predominant compounds by mass spectrometry. In plate assay, punicalagin (10 mg/mL) was active with hazy inhibition zones of 17, 14, and 13 mm against S. typhi, S. typhimurium and E. coli, respectively. However, in broth dilution assay punicalagin showed no MIC up to 10 mg/mL. The SICs 30 μg, 100 μg, and 500 μg of punicalagin combined with antimicrobials i.e., aminoglycoside, β-lactam, and fluoroquinolone act in synergy against MDR strains with % increase in inhibition zone values varying from 3.4 ± 2.7% to 73.8 ± 8.4%. In time-kill curves, a significant decrease in cell density was observed with the SICs of antimicrobials/punicalagin (0.03–60 μg/mL/30, 100, 500 μg/mL of punicalagin) combinations. Conclusions The P. granatum peel methanol extract exhibited antimicrobial activity against MDR Enterobacteriaceae pathogens. Punicalagin, the bacteriostatic flavonoid act as a concentration-dependent sensitizing agent for antimicrobials against Enterobacteriaceae. Our findings for the therapeutic punicalagin-antimicrobial combination prompt further evaluation of punicalagin as a potent activator for drugs, which otherwise remain less or inactive against MDR strains.

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BACTERIAL INHIBITORY EFFECT OF ALGERIAN POMEGRANATE (Punica Granatum L.) EXTRACTS (PEEL, JUICE, AND SEED) AGAINST MULTIDRUG RESISTANT BACTERIA

Cited by 4 publications

References 19 publications

In vitro Antioxidant, anti-Alzheimer and Antibacterial Activities of Ethyl acetate and n-Butanol Fractions of Punica granatum Peel from Algeria

In vitro Antioxidant, anti-Alzheimer and Antibacterial Activities of Ethyl acetate and n-Butanol Fractions of Punica granatum Peel from Algeria

Pomegranate Seeds and Peel Ethanolic Extracts Anticancer Potentials and Related Genetic, Histological, Immunohistochemical, Apoptotic and Oxidative Stress Profiles: In vitro Study

Punicalagin, a pomegranate polyphenol sensitizes the activity of antibiotics against three MDR pathogens of the Enterobacteriaceae

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