Bacterial invasion into dentinal tubules of human vital and nonvital teeth

Nagaoka, Sumiharu; Miyazaki, Youichi; Liu, Hong-Jih; Iwamoto, Yumiko; Kitano, Motoo; Kawagoe, Mikio

doi:10.1016/s0099-2399(06)81098-8

Cited by 87 publications

(63 citation statements)

References 17 publications

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“…Another advantage of the smear layer is the ability to reduce the permeability of debris to the dentinal tubules (KIM et al, 2002). A smear layer of small particle size prevents bacteria from penetrating the dentine in studies of human teeth (NAGOAKA et al, 1995). Studies carried out in extracted human teeth have shown that dentinal tubules are the major conduits for fluid diffusion across dentin (PASHLEY, 1992;HOLLAND, 1994).…”

Section: Discussionmentioning

confidence: 99%

Effect of ultrasonic scaling of teeth involved with periodontal disease in dogs: a scanning electron microscopic study

Lacerda

Alessi

2015

Biosci. J.

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Scaling using ultrasonic dental equipment was performed on 20 teeth extracted from dogs with advanced periodontal disease, under clinical indication. The surgical extraction of the teeth, which already showed mobility, carried out by means of a lever, tried not to touch the radicular surface which would be assessed. The mesial surface of the cervical root region was used for verifying the effect of the ultrasonic scaling and the distal surface was maintained intact for control. After treatment, the teeth were sectioned, separating the region of interest, and these were fixed in glutaraldehyde, dried with a critical point dryer and metallized for observation under electronic scanning microscopy. The presence of plaque, biofilm and rod cells on the non-treated surface was quite characterized. On the surface submitted to treatment by ultrasound, it was possible to see areas with the removal of all the dental calculus, and areas with exposed naked dentine and showing open dentinal tubules. It was possible to observe also the occasional presence of bio-film and rod cells penetrating the dentinal tubules. No harm to the dentine was observed. Thus, ultrasonic scaling showed to be useful in the purpose of removing plaque and can be better developed. Future studies are necessary to establish a comparison with other methods of scaling.

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Section: Discussionmentioning

confidence: 99%

Effect of ultrasonic scaling of teeth involved with periodontal disease in dogs: a scanning electron microscopic study

Lacerda

Alessi

2015

Biosci. J.

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“…Although vital teeth are more resistant to bacterial invasion into dentinal tubules than are non-vital teeth (Nagaoka & others), 42 and the outward fluid movement retards the inward diffusion of bacterial toxins (Pashley & others), 41 mechanically-exposed dentin requires provisionalization to reduce sensitivity and bacterial microleakage (Cagidiaco & others). 5 It has been stated that tooth sensitivity related to preparation is profound in the first week but decreases with time (Lundy & Stanley).…”

Section: Discussionmentioning

confidence: 99%

“…35 Some researchers suggested use of acidic conditioners (Watanabe & others) 31 or degreasing agents (Mojon & others) 33 [36][37][38][39][40] Compared to the conventional method, in which the dentin-bonding agent is applied during the final luting procedure, this method requires hybridization of the exposed dentin surface immediately after tooth preparation and hybridization during final luting procedures (Bertschinger & others; Paul & Scharer). [36][37] The primary advantage of this technique is to protect the tooth from the consequences of microleakage by sealing the dentin tubules that are vulnerable to bacterial invasion (Cox & others; Pashley & others; Nagaoka & others; Cox) 38,[41][42][43] immediately after completion of the preparation. Sealing of the dentin tubules also reduces sensitivity (Cagidiaco & others) 5 by preventing hydraulic fluid flow within the dentin tubules, which is associated with post-operative sensitivity (Brännström; Suzuki, Cox & White; Pashley & others).…”

Section: Introductionmentioning

confidence: 99%

“…[44][45][46] It has been shown that cements can be forced into dentin tubules before the luting agent sets (Zaimoglu & Aydin) 47 and microorganisms and their by-products can penetrate into the patent dental tubules post-operatively (Lundy & Stanley; Nagaoka & others). 42,48 Therefore, the early sealing of dentin tubules also may prevent occlusion of dental tubules by PLA remnants and prevent eugenol diffusion across dentin, which may later inhibit polymerization of the bonding agents (Bertschinger & others). 36 In this study, two null hypotheses were tested.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Previous Provisional Cementation on the Bond Strength Between Two Definitive Resin-based Luting and Dentin Bonding Agents and Human Dentin

Erkut

Küçükeşmen²,

Eminkahyagil³

et al. 2007

Operative Dentistry

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Clinical RelevanceBased on in vitro results in extracted teeth, the dual bonding method used was effective in restoring the shear bond strength values that decreased after applying provisional luting agent regardless of the composition of the provisional luting agent (eugenol-based or eugenolfree) used. SUMMARYThis study evaluated the effect of two different types of provisional luting agents (RelyX Temp E, eugenol-based; RelyX Temp NE, eugenol-free) on the shear bond strengths between human dentin and two different resin-based luting systems (RelyX ARC-Single Bond and Duo Link-One Step) after cementation with two different techniques (dual bonding and conventional technique). One hundred human molars were trimmed parallel to the original long axis, to expose flat dentin surfaces, and were divided into three groups. After related surface treatments for each specimen, the resin-based luting agent was applied in a silicone cylindrical mold (3.5 x 4 mm), placed on the bonding-agent-treated dentin surfaces and polymerized. In the control group (n=20), the specimens were further divided into two groups (n=10), and two different resin-based luting systems were immediately applied following the manufacturer's protocols: RelyX ARC-Single Bond (Group I C ) and Duo Link-One Step (Group II C). In the provisionalization group (n=40), the specimens were further divided into four subgroups of 10 specimens each (Group I N, I E and Group II N, II E). In Groups I N and II N, eugenolfree (RelyX NE), and in groups I E and II E, eugenol-based (RelyX E) provisional luting Operative Dentistry, 2007, 32-1, 84-93 agents (PLA), were applied on the dentin surface. The dentin surfaces were cleaned with a flourfree pumice, and the resin-based luting systems RelyX ARC (Group I N and E) and Duo Link (Group II N and E) were applied. In the Dual bonding groups (n=40), the specimens were divided into four subgroups of 10 specimens each (Group I ND, ED and Group II ND, ED). The specimens were treated with Single Bond (Groups I ND and ED) or One Step (Groups II ND and ED). After the dentin bonding agent treatment, RelyX Temp NE was applied to Groups I ND and II ND, and RelyX Temp E was applied to Groups I ED and II ED. The dentin surfaces were then cleaned as described in the provisionalization group, and the resin-based luting systems were applied: RelyX ARC-Single Bond (Group I ND and ED) and Duo Link-One Step (Group II ND and ED). After 1,000 thermal cycles between 5°C and 55°C, shear bond testing was conducted at a crosshead speed of 0.5 mm/minutes. One-way ANOVA, followed by a post hoc Tukey test (α α=0.05) was done. The dentin-resin-based luting system interfaces were evaluated under a scanning electron microscope. There was a significant reduction in the mean shear bond strength values of groups subjected to the provisionalization compared to the control and dual bonding technique groups (p<0.05). The composition of provisional luting did not create a significant difference with regard to reducing shear bond strength values (p>0.05). Wi...

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“…This was related to formation of longer resin tags and a thicker hybrid zone. Moreover, IDS method can protect the tooth from the consequences of microleakage by sealing the dentin tubules that are vulnerable to bacteria invasion, immediately after completion of the preparation 16) . Sealing of the dentin tubules also reduces sensitivity by preventing hydraulic fluid flow within the dentin tubules, which is associated with postoperative sensitivity 17) .…”

Section: ⅳ Discussionmentioning

confidence: 99%

The effect of various bonding systems on the microtensile bond strength of immediate and delayed dentin sealing

Kim

Hur

et al. 2008

J Korean Acad Conserv Dent

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Bacterial invasion into dentinal tubules of human vital and nonvital teeth

Cited by 87 publications

References 17 publications

Effect of ultrasonic scaling of teeth involved with periodontal disease in dogs: a scanning electron microscopic study

Effect of ultrasonic scaling of teeth involved with periodontal disease in dogs: a scanning electron microscopic study

Influence of Previous Provisional Cementation on the Bond Strength Between Two Definitive Resin-based Luting and Dentin Bonding Agents and Human Dentin

The effect of various bonding systems on the microtensile bond strength of immediate and delayed dentin sealing

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