Bacterial Invasion of Pulpal Dentin Wall in vitro

Akpata, E.S.; Blechman, Harry

doi:10.1177/00220345820610021401

Cited by 126 publications

(99 citation statements)

References 3 publications

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“…By contrast to results obtained with gram-positive bacteria, monocultures of gram-negative anaerobic bacteria do not invade dentinal tubules (1,37). This has led to the notion that dentinal tubules may in some way be selective for streptococci and other facultatively anaerobic bacteria.…”

mentioning

confidence: 80%

“…Previous light microscopy studies have demonstrated that several gram-negative anaerobic bacterial species, such as Prevotella melananogenica (1) and Prevotella intermedia (37), were unable to invade dentin. On the other hand, it has been reported that Porphyromonas endodontalis BN11a-f and P. gingivalis ATCC 33277 in monoculture were able to invade bovine dentinal tubules, although very few tubules were infected (40).…”

Section: Discussionmentioning

confidence: 99%

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Coinvasion of Dentinal Tubules by Porphyromonas gingivalis and Streptococcus gordonii Depends upon Binding Specificity of Streptococcal Antigen I/II Adhesin

et al. 2000

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Cell wall-anchored polypeptides of the antigen I/II family are produced by many species of oral streptococci. These proteins mediate adhesion of streptococci to salivary glycoproteins and to other oral microorganisms and promote binding of cells to collagen type I and invasion of dentinal tubules. Since infections of the root canal system have a mixed anaerobic bacterial etiology, we investigated the hypothesis that coadhesion of anaerobic bacteria with streptococci may facilitate invasive endodontic disease. Porphyromonas gingivalis ATCC 33277 cells were able to invade dentinal tubules when cocultured with Streptococcus gordonii DL1 (Challis) but not when cocultured with Streptococcus mutans NG8. An isogenic noninvasive mutant of S. gordonii, with production of SspA and SspB (antigen I/II family) polypeptides abrogated, was deficient in binding to collagen and had a 40% reduced ability to support adhesion of P. gingivalis. Heterologous expression of the S. mutans SpaP (antigen I/II) protein in this mutant restored collagen binding and tubule invasion but not adhesion to P. gingivalis or the ability to promote P. gingivalis coinvasion of dentin. An isogenic afimbrial mutant of P. gingivalis had 50% reduced binding to S. gordonii cells but was unaffected in the ability to coinvade dentinal tubules with S. gordonii wild-type cells. Expression of the S. gordonii SspA or SspB polypeptide on the surface of Lactococcus lactis cells endowed these bacteria with the abilities to bind P. gingivalis, penetrate dentinal tubules, and promote P. gingivalis coinvasion of dentin. The results demonstrate that collagen-binding and P. gingivalis-binding properties of antigen I/II polypeptides are discrete functions. Specificity of antigen I/II polypeptide recognition accounts for the ability of P. gingivalis to coinvade dentinal tubules with S. gordonii but not with S. mutans. This provides evidence that the specificity of interbacterial coadhesion may influence directly the etiology of pulpal and periapical diseases.

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mentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Coinvasion of Dentinal Tubules by Porphyromonas gingivalis and Streptococcus gordonii Depends upon Binding Specificity of Streptococcal Antigen I/II Adhesin

et al. 2000

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“…Although most of follow-up studies on endodontic therapy were reported overall success rate of 85% to 90%, the main cause of root canal therapy failure being persistent microorganisms [11,12]. Enterococcus faecalis is one of the most resistant microorganisms found in case of failed root canal therapy [13][14][15]. It is difficult to remove the smear layer, creating condition for the formation of E. faecalis biofilm, in periapical region by using current routine endodontic procedures that depend on chemo-mechanical debridement.…”

Section: Discussionmentioning

confidence: 99%

Multiple Applications with Er:YAG Laser in Apicoectomy - A Clinical Case Report

Yu¹

2017

Dentistry

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The setting was 50 mJ/10 Hz with 100% of water for full mouth laser scaling and then ultrasonic scaling tip was used for supragingival scaling. In addition, another laser setting was applied at 50 mJ/30Hz with 100% of water for subgingival sterilization and then ultrasonic round tip was used for subgingival curettage [7,8]. Step 2. Vertical soft tissue incisionThe setting was 100 mJ/40 Hz with 100% of water for incision [in vestibular near apex of #21] (Figure 3). Step 3. Granulation tissues ablationThe setting was 400 mJ/15Hz with 100% of water. Granulation tissues were ablation thoroughly (Figures 4 and 5).

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“…Not all bacteria can be removed, and pulp tissue remnants and bacteria can invade the dentin even if the root canal is prepared with the utmost caution. One report suggested that no cleaning effect is exerted on the root canal wall because it is protected by a smear layer 1) . Root canal therapy is thus dependent on the pharmacological effects of intracanal medications in addition to adequate canal preparation.…”

Section: Introductionmentioning

confidence: 99%

Current Trends in Use of Intracanal Medications in Dental Care Facilities: Questionnaire-based Survey on Training Dental Hygienists at Educational Institutions

Furusawa

Yoshida

Hosokawa

et al. 2013

Bull. Tokyo Dent. Coll.

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The success of root canal therapy is dependent not only on removal of infected pulp (pulpectomy) followed by root canal enlargement, but also on the pharmacological effects of intracanal medications. Various intracanal medications are used. Formaldehyde preparations such as formocresol were common in the past, but these are no longer used in Europe or the US due to the biological toxicity of formaldehyde. In this study, a questionnaire was used to determine current trends in the use of intracanal medications at dental care facilities where dental hygiene students undergo practical training. The questionnaire comprised questions regarding the types of frequently used intracanal medications and their methods of application at dental care facilities in Saitama and Shizuoka prefectures. The results indicated that calcium hydroxide preparations were more commonly used in Europe or the US. However, these results also revealed that formaldehyde preparations were frequently used, which slightly differs from the scenario in Europe and the US. This study revealed that multiple intracanal medications were used for root canal therapy. Furthermore, it was also observed that cotton plugs were generally used as applicator tips for intracanal medications, whereas the use of absorbent paper points was relatively uncommon. The results suggest that the cost of absorbent paper points needs to be reduced.

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Bacterial Invasion of Pulpal Dentin Wall in vitro

Cited by 126 publications

References 3 publications

Coinvasion of Dentinal Tubules by Porphyromonas gingivalis and Streptococcus gordonii Depends upon Binding Specificity of Streptococcal Antigen I/II Adhesin

Coinvasion of Dentinal Tubules by Porphyromonas gingivalis and Streptococcus gordonii Depends upon Binding Specificity of Streptococcal Antigen I/II Adhesin

Multiple Applications with Er:YAG Laser in Apicoectomy - A Clinical Case Report

Current Trends in Use of Intracanal Medications in Dental Care Facilities: Questionnaire-based Survey on Training Dental Hygienists at Educational Institutions

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