Bacterial Isolates of Urine and their Susceptibility to Antimicrobials

Mahdi, Batool Mutar; Khudhur, Haneen Basim; Abdul-Hussein, Mustafa Mohammad

doi:10.3889/oamjms.2020.4086

Cited by 5 publications

(10 citation statements)

References 23 publications

(25 reference statements)

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“…In the current study, the most common bacterium isolated from human urine was E. coli, which constituted 85.7% of the enteric bacteria isolated and 26.1% of all bacterial isolates (19). This result is in agreement with another study in Iraq (18). The same organism was clearly documented to be the major cause of UTI worldwide.…”

Section: Discussionsupporting

confidence: 93%

“…Among various diseases caused by E. coli, urinary tract infections (UTIs) commonly occur. In the current study, the females affected with UTIs outnumbered the males either in human or cattle; this is consistent with other researchers (17,18). In the current study, the most common bacterium isolated from human urine was E. coli, which constituted 85.7% of the enteric bacteria isolated and 26.1% of all bacterial isolates (19).…”

Section: Discussionsupporting

confidence: 92%

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RAPD-PCR and phylogenetic analysis of E. coli isolated from human and cattle urinary tract infections

Mraidi¹,

Lafta²

2021

IJVS

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This study aimed at isolating uropathogenic Escherichia coli from urinary tract infections (UTIs) of human and cattle to examine the molecular diversity and phylogenetic relationship of the isolates. A total of 100 urine samples were collected from UTIs of human and cattle. The isolates identification was done using routine diagnostic methods and confirmed by Vitek2. Antimicrobial susceptibility was tested against 10 antimicrobials. Random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) was applied to identify the genetic diversity among E. coli isolates from human and animal origin by using five different octamer primers. The gelJ software for the phylogenetic analysis created Dendrograms. Out of 50 human urine samples, E. coli was isolated from 12 (24%) samples, and was positive in 5 out of 50 (10%) of cattle urine samples. Concerning the antimicrobial susceptibility test, both human and animal isolates revealed rather approximate results when tested mainly against imipenem, cefotaxime, and ciprofloxacin. These antimicrobial data might indicate presence of a degree of similarity between the human and animal isolates. Using RAPD-PCR, three of the primers produced polymorphic bands; therefore, they were used for further analysis of the results. Either of P1, P3 or P4 primers showed presence of similarity between human and cow isolates. To conclude, RAPD-PCR and gelJ software might be of attractive use to identify and analyze the occurrence of genetic relationships, as this could assist in controlling the routes and sources of infection transmission between human and animal in order to prevent zoonotic infections.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

RAPD-PCR and phylogenetic analysis of E. coli isolated from human and cattle urinary tract infections

Mraidi¹,

Lafta²

2021

IJVS

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“…UTI occurs in all people of all ages, but the rate of positive urine cultures in this study is much higher in females (78.8%) because of the high prevalence of UTI among them. The same finding is well documented in other studies (Mahdi et al, 2020;Mohamed Hayir et al, 2019). This is referring to differences in female anatomy, short urethra and its proximity to the anus, sexual activity, moisture environment of the urogenital area let bacteria multiply and become more ready to reach the urinary system (Gupta et al, 2019;Jatileni et al, 2015;Mohammed et al, 2016).…”

Section: Discussionsupporting

confidence: 89%

Bacterial Causes and their Antimicrobial Susceptibility Testing among Urinary Tract Infection Patients in Tobruk Area, Libya

Mostafa

Mustafa

Kumar

2021

MJSc

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Urinary tract infections are very common among people of all ages, but the disease is more prevalent in women, so proper clinical and laboratory diagnosis, and the right treatment are very important to avoid complications and antimicrobial resistance. This study aimed to determine the bacterial causes and antibiotic susceptibility patterns of UTI patients. Recorded results of 6065 urine sample cultures and their antimicrobial susceptibility tests from the Department of Microbiology in Tobruk Medical Center, Tobruk City, were obtained from September 2016 to December 2018. The data was analyzed and discussed in compare with other studies. The study showed that a UTI is more common in females (78.8%) compared with male patients (21.2%). Gram-negative bacteria accounted for the majority of urinary pathogens (90%), where E. coli alone was (58.4%), klebsiellae (17.4%), Proteus (10.7%), while Pseudomonas species was the least common (3.3%) of the total. While the Gram-positive bacteria S. aureus constituted 9.6% of the total. The study was found statistically significant (P=0.000). The most effective antibiotics against all uropathogens were Imipenem and Amikacin, and less effective antibiotics were Ampicillin and Amoxicillin-clavulanic acid. This study concluded that the most common cause of UTI in Tobruk was Escherichia coli and the most effective antibiotics appear to be Imipenem and Amikacin.

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“…It is between the most frequent bacteriological infections in both men and women, with a feminine predisposition. Antibiotics are frequently used to detect and cure infectious diseases; however, some bacteria have evolved the ability to thrive in an antibacterial drug environment, resulting in “antibiotic resistance.” Sexual activity, vaginal infections, antimicrobial activities of prostatic fluids, diabetes, hygiene practices, obesity, and genetic predisposition are all risk factors for UTIs [ 1 ]. The diagnosis is made by combining a urine culture with both the existence of clinical symptoms, and antimicrobial sensitivity is assessed by diffusion method, dilution testing, and the death curve.…”

Section: Introductionmentioning

confidence: 99%

“…The greatest category of specimens studied in healthcare microbiology laboratories is urine collection microscopy, cultures, and sensitivities that offer verification in the medicinal utilization of antibiotics. Furthermore, across all areas of healthcare administration, the clinical perception of UTI seems to be the second most popular criterion for empirical antibiotic therapy [ 1 ]. The growth in uropathogenic microorganisms resistant to presently used antibiotics is a distinct major concern as in empirical utilization of antibiotics among patients with UTI around the world.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Bacterial Isolates from the Urine Specimens of Urinary Tract Infected Patient

Rao¹,

Vennila

Kosanam

et al. 2022

BioMed Research International

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Urinary tract infections (UTIs) maintained a serious public health concern, as did the growth in antibiotic resistance both between uropathogenic microorganisms. A regular assessment of the microbiological agents that cause UTIs, as well as their antimicrobial resistance, is essential for a tailored empirical antibiotic response. Knowing the variables that cause UTIs can help you intervene quickly and simply to get the condition under control. The most common infecting species in acute infection is Escherichia coli (E. coli). To strengthen infection control strategies, it is necessary to know the prevalence and location of UTI. The goal of this research is to measure the frequency of microorganisms isolated from patients with UTIs as well as the antimicrobial sensitivity characteristics of Gram-negative bacteria. The purpose of this research has been to evaluate the frequency of UTIs by extracting and characterizing the various bacterial etiological organisms, as well as to assess the factors linked to UTIs. The goal of this research is to identify, characterize, and establish the antibiotic susceptibility patterns of bacteria linked to urinary tract infections. Fresh collected urine specimen was taken from inpatients or outpatients in UTI cases and bacteriologically tested using conventional microbiological methods. The Kirby-Bauer disc diffusion method was used to create the antibiogram. Staphylococcus saprophyticus, Staphylococcus aureus (28%), and Escherichia coli (24.6%) were the most common isolates (20%). The evaluated agents’ antibacterial activity was all in the following order: cefixime, ciprofloxacin, augmentin, gentamicin, ceftazidime, nitrofurantoin, ofloxacin, and cefuroxime. It was discovered that each and every one of the microbes exhibited varied degrees of resistance to the antibiotics nitrofurantoin, ciprofloxacin, and ofloxacin. Every type of bacteria, with the exception of those belonging to the genus Streptococcus, has a Multiple Antibiotic Resistance Index (MARI) that is more than 0.2. The first-line therapies for urinary tract infections (UTIs) in the region would consist of ciprofloxacin, ofloxacin, and nitrofurantoin. Lower urinary tract infections almost never result in problems if they are diagnosed and treated as soon as possible and in the correct manner. However, if treatment is not sought, a urinary tract infection can lead to serious complications.

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Bacterial Isolates of Urine and their Susceptibility to Antimicrobials

Cited by 5 publications

References 23 publications

RAPD-PCR and phylogenetic analysis of E. coli isolated from human and cattle urinary tract infections

RAPD-PCR and phylogenetic analysis of E. coli isolated from human and cattle urinary tract infections

Bacterial Causes and their Antimicrobial Susceptibility Testing among Urinary Tract Infection Patients in Tobruk Area, Libya

Assessment of Bacterial Isolates from the Urine Specimens of Urinary Tract Infected Patient

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