Bacterial leaf and peduncle soft rot caused byPectobacterium carotovorum on tulips in Konya, Turkey

Boyraz, Nuh; Baştaş, Kubilay Kurtuluş; Maden, Salih; Yasar, Aysegul

doi:10.1007/bf02980956

Cited by 14 publications

(8 citation statements)

References 6 publications

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“…Farmers in these areas keep their seed tubers in indoor stores annually and, therefore, tuber contamination with pectolytic bacteria is one of the main causes of postharvest losses in this crop. The occurrence of pectolytic enterobacteria causing soft rot and leaf spot of ornamental plants have been previously reported in many countries (Chao et al 2006;Boyraz et al 2006;Kim and Kim 2007;Alippi and Lopez 2009), including greenhouses of northern Iran (Khalemlou 2007).…”

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confidence: 98%

Characterization of Pectobacterium species from Iran using biochemical and molecular methods

et al. 2010

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Characteristics of forty strains from macerated potato tubers and water-soaked lesions of some ornamental plants were studied in north parts of Iran. The causal organisms isolated from infected tissues were identified as Pectobacterium spp. based on their physiological and biochemical assays and confirmed by species and subspecies specific PCR and RFLP analysis of 16S-23S intergenic transcribed spacer region. Artificial inoculation of isolates to their related hosts generated the same symptoms on potato and ornamental plants, from which the same bacteria were isolated and identified. We detected two groups of atypical isolates in this study. The first group from potato classified as Pectobacterium carotovorum subsp. carotovorum by phenotypic tests but was unable to elicit HR on tobacco leaves, to grow at 37°C and to amplify the pel gene relevant to this subspecies. The second one from ornamental plants which was again characterized as Pectobacterium carotovorum subsp. carotovorum in biochemical assays, produced a unique ITS-RFLP profile different from all of known Pectobacterium species and subspecies. Our findings based on phylogenetic analysis using concatenated partial sequences of housekeeping genes mdh and gapA, indicated the occurrence of P. wasabiae as a novel species in potato storage in Iran. Furthermore we detected a distinct clade of Pectobacterium spp. from some ornamental plants including Schlumbergera bridgesii, Syngonium podophyllum and Iris spp.

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confidence: 98%

Characterization of Pectobacterium species from Iran using biochemical and molecular methods

et al. 2010

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“…Pcc is a well-described Gram-negative bacterium known to cause soft rot in various plants including ornamentals such as hyacinth, tulip, and narcissus. Pcc can cause a dramatic loss under favorable conditions if it is not controlled properly ( Boyraz et al, 2006 , Van Doorn et al, 2008 ). The promising data found for vegetable seeds motivated us to test whether the ozonated water is effective in disinfecting the bulbs from Pcc .…”

Section: Discussionmentioning

confidence: 99%

Ozone treatment inactivates common bacteria and fungi associated with selected crop seeds and ornamental bulbs

Çetinkaya

Pazarlar

Paylan

2022

Saudi Journal of Biological Sciences

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“…The occurrence of pectolytic enterobacteria causing soft rot have been previously reported in different plants such as radish (Goto and Matsumoto, 1987), aglaonema (Chao et al, 2006), dieffenbachia (MacFadden, 1961Nieves-Brun et al, 1985), tulip (Boyraz et al, 2006), syngonium (Alippi and Lopez, 2009), potato, tomato, carrot and cabbage (Maisuria and Nerurkar, 2013), artichoke (Loreti et al, 2001), sweet potato, chinese cabbage and eggplant (Golkhandan et al, 2013). The key objective of this research was to identify Pectobacterium spp.…”

Section: Introductionmentioning

confidence: 95%

Characterization of Pectobacterium species isolated from vegetable crops in north-west of Iran

Rafiei¹,

Khodakaramian²,

Ravari³

2015

Afr. J. Agric. Res.

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In August 2012, vegetable crops including potato, cabbage, bell pepper and carrot showing symptoms of maceration and water soaked lesions on their tuber, leaf and fruit were collected from major vegetable growing areas in northwest of Iran. Physiological and biochemical assays divided the isolates into two main groups according to their ability to grow at 37°C. In addition, these two groups were differentiated by some biochemical characteristics such as utilization of α-methyl glucoside and maltose, reducing substrates from sucrose and gelatin liquefaction. Partial sequence of polymerase chain reaction (PCR) product from reaction of putative Pectobacterium spp. with 16S rRNA confirmed the results obtained from physiological and biochemical assays used for identification of the bacterium. All of the causal organisms isolated from infected tissues were identified as Pectobacterium spp. based on biochemical characteristics and PCR amplification of the 16S rRNA gene with specific primers PCCSSF and PCCSSR. Twenty of the isolates were identified as Pectobacterium carotovorum subsp. carotovorum using a polyphasic approach including physiological, biochemical and molecular characteristics. Only six isolates from infected bell peppers and five isolates from infected potatoes identified as Pectobacterium wasabiae and Pectobacterium atrosepticum respectively. Our findings based on physiological, biochemical and molecular assays indicated the occurrence of P. wasabiae as a novel species in bell pepper in Iran. Moreover, genetic diversity of Pcc strains in this study was also performed based on Repetitive extragenic palindromic-PCR (rep-PCR). High genetic variability was revealed among these Pcc strains by rep-PCR typing using two primers sets for ERIC-PCR and BOX-PCR.

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Bacterial leaf and peduncle soft rot caused byPectobacterium carotovorum on tulips in Konya, Turkey

Cited by 14 publications

References 6 publications

Characterization of Pectobacterium species from Iran using biochemical and molecular methods

Characterization of Pectobacterium species from Iran using biochemical and molecular methods

Ozone treatment inactivates common bacteria and fungi associated with selected crop seeds and ornamental bulbs

Characterization of Pectobacterium species isolated from vegetable crops in north-west of Iran

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